Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes

Malignant melanoma is responsible for the majority of skin cancer-related deaths. The methods of cancer treatment include surgical removal, chemotherapy, immunotherapy, and targeted therapy. However, neither of these methods gives satisfactory results. Therefore, the development of new anticancer th...

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Autores principales:	Klaudia Banach, Justyna Kowalska, Zuzanna Rzepka, Artur Beberok, Jakub Rok, Dorota Wrześniok
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	melanotic melanoma UVA radiation ketoprofen melanocytes COLO829 Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e3ea8656673249caa07738f09854b431
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spelling	oai:doaj.org-article:e3ea8656673249caa07738f09854b4312021-11-11T17:23:18ZKetoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes10.3390/ijms2221119661422-00671661-6596https://doaj.org/article/e3ea8656673249caa07738f09854b4312021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11966https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Malignant melanoma is responsible for the majority of skin cancer-related deaths. The methods of cancer treatment include surgical removal, chemotherapy, immunotherapy, and targeted therapy. However, neither of these methods gives satisfactory results. Therefore, the development of new anticancer therapeutic strategies is very important and may extend the life span of people suffering from melanoma. The aim of this study was to examine the effect of ketoprofen (KTP) and UVA radiation (UVAR) therapy on cell proliferation, apoptosis, and cell cycle distribution in both melanotic melanoma cells (COLO829) and human melanocytes (HEMn-DP) in relation to its supportive effect in the treatment of melanoma. The therapy combining the use of pre-incubation with KTP and UVAR causes a significant increase in the anti-proliferative properties of ketoprofen towards melanoma cells and the co-exposure of melanotic melanoma cells induced apoptosis shown as the mitochondrial membrane breakdown, cell-cycle deregulation, and DNA fragmentation. Moreover, co-treatment led to GSH depletion showing its pro-apoptotic effect dependent on ROS overproduction. The treatment did not show a significant effect on normal cells—melanocytes—which indicates its high selectivity. The results suggest a possible benefit from the use of the ketoprofen and ultraviolet A irradiation as a new concept of melanotic melanoma therapy.Klaudia BanachJustyna KowalskaZuzanna RzepkaArtur BeberokJakub RokDorota WrześniokMDPI AGarticlemelanotic melanomaUVA radiationketoprofenmelanocytesCOLO829Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11966, p 11966 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melanotic melanoma UVA radiation ketoprofen melanocytes COLO829 Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	melanotic melanoma UVA radiation ketoprofen melanocytes COLO829 Biology (General) QH301-705.5 Chemistry QD1-999 Klaudia Banach Justyna Kowalska Zuzanna Rzepka Artur Beberok Jakub Rok Dorota Wrześniok Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes
description	Malignant melanoma is responsible for the majority of skin cancer-related deaths. The methods of cancer treatment include surgical removal, chemotherapy, immunotherapy, and targeted therapy. However, neither of these methods gives satisfactory results. Therefore, the development of new anticancer therapeutic strategies is very important and may extend the life span of people suffering from melanoma. The aim of this study was to examine the effect of ketoprofen (KTP) and UVA radiation (UVAR) therapy on cell proliferation, apoptosis, and cell cycle distribution in both melanotic melanoma cells (COLO829) and human melanocytes (HEMn-DP) in relation to its supportive effect in the treatment of melanoma. The therapy combining the use of pre-incubation with KTP and UVAR causes a significant increase in the anti-proliferative properties of ketoprofen towards melanoma cells and the co-exposure of melanotic melanoma cells induced apoptosis shown as the mitochondrial membrane breakdown, cell-cycle deregulation, and DNA fragmentation. Moreover, co-treatment led to GSH depletion showing its pro-apoptotic effect dependent on ROS overproduction. The treatment did not show a significant effect on normal cells—melanocytes—which indicates its high selectivity. The results suggest a possible benefit from the use of the ketoprofen and ultraviolet A irradiation as a new concept of melanotic melanoma therapy.
format	article
author	Klaudia Banach Justyna Kowalska Zuzanna Rzepka Artur Beberok Jakub Rok Dorota Wrześniok
author_facet	Klaudia Banach Justyna Kowalska Zuzanna Rzepka Artur Beberok Jakub Rok Dorota Wrześniok
author_sort	Klaudia Banach
title	Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes
title_short	Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes
title_full	Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes
title_fullStr	Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes
title_full_unstemmed	Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes
title_sort	ketoprofen combined with uva irradiation exerts higher selectivity in the mode of action against melanotic melanoma cells than against normal human melanocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e3ea8656673249caa07738f09854b431
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Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes

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