Structural variation among wild and industrial strains of Penicillium chrysogenum.

Strain selection and strain improvement are the first, and arguably most important, steps in the industrial production of biological compounds by microorganisms. While traditional methods of mutagenesis and selection have been effective in improving production of compounds at a commercial scale, the...

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Autores principales:	Valerie L Wong, Christopher E Ellison, Michael B Eisen, Lior Pachter, Rachel B Brem
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3ed4f3f67f840f39e6e7dc0494b7194
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spelling	oai:doaj.org-article:e3ed4f3f67f840f39e6e7dc0494b71942021-11-18T08:19:29ZStructural variation among wild and industrial strains of Penicillium chrysogenum.1932-620310.1371/journal.pone.0096784https://doaj.org/article/e3ed4f3f67f840f39e6e7dc0494b71942014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24824901/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Strain selection and strain improvement are the first, and arguably most important, steps in the industrial production of biological compounds by microorganisms. While traditional methods of mutagenesis and selection have been effective in improving production of compounds at a commercial scale, the genetic changes underpinning the altered phenotypes have remained largely unclear. We utilized high-throughput Illumina short read sequencing of a wild Penicillium chrysogenum strain in order to make whole genome comparisons to a sequenced improved strain (WIS 54-1255). We developed an assembly-free method of identifying chromosomal rearrangements and validated the in silico predictions with a PCR-based assay and Sanger sequencing. Despite many rounds of mutagen treatment and artificial selection, WIS 54-1255 differs from its wild progenitor at only one of the identified rearrangements. We suggest that natural variants predisposed for high penicillin production were instrumental in the success of WIS 54-1255 as an industrial strain. In addition to finding a previously published inversion in the penicillin biosynthesis cluster, we located several genes related to penicillin production associated with these rearrangements. By comparing the configuration of rearrangement events among several historically important strains known to be high penicillin producers to a collection of recently isolated wild strains, we suggest that wild strains with rearrangements similar to those in known high penicillin producers may be viable candidates for further improvement efforts.Valerie L WongChristopher E EllisonMichael B EisenLior PachterRachel B BremPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96784 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valerie L Wong Christopher E Ellison Michael B Eisen Lior Pachter Rachel B Brem Structural variation among wild and industrial strains of Penicillium chrysogenum.
description	Strain selection and strain improvement are the first, and arguably most important, steps in the industrial production of biological compounds by microorganisms. While traditional methods of mutagenesis and selection have been effective in improving production of compounds at a commercial scale, the genetic changes underpinning the altered phenotypes have remained largely unclear. We utilized high-throughput Illumina short read sequencing of a wild Penicillium chrysogenum strain in order to make whole genome comparisons to a sequenced improved strain (WIS 54-1255). We developed an assembly-free method of identifying chromosomal rearrangements and validated the in silico predictions with a PCR-based assay and Sanger sequencing. Despite many rounds of mutagen treatment and artificial selection, WIS 54-1255 differs from its wild progenitor at only one of the identified rearrangements. We suggest that natural variants predisposed for high penicillin production were instrumental in the success of WIS 54-1255 as an industrial strain. In addition to finding a previously published inversion in the penicillin biosynthesis cluster, we located several genes related to penicillin production associated with these rearrangements. By comparing the configuration of rearrangement events among several historically important strains known to be high penicillin producers to a collection of recently isolated wild strains, we suggest that wild strains with rearrangements similar to those in known high penicillin producers may be viable candidates for further improvement efforts.
format	article
author	Valerie L Wong Christopher E Ellison Michael B Eisen Lior Pachter Rachel B Brem
author_facet	Valerie L Wong Christopher E Ellison Michael B Eisen Lior Pachter Rachel B Brem
author_sort	Valerie L Wong
title	Structural variation among wild and industrial strains of Penicillium chrysogenum.
title_short	Structural variation among wild and industrial strains of Penicillium chrysogenum.
title_full	Structural variation among wild and industrial strains of Penicillium chrysogenum.
title_fullStr	Structural variation among wild and industrial strains of Penicillium chrysogenum.
title_full_unstemmed	Structural variation among wild and industrial strains of Penicillium chrysogenum.
title_sort	structural variation among wild and industrial strains of penicillium chrysogenum.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e3ed4f3f67f840f39e6e7dc0494b7194
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Structural variation among wild and industrial strains of Penicillium chrysogenum.

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