An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling

Abstract PHRF1 is involved in transforming growth factor β (TGF-β) signaling to constrain the formation of acute promyelocytic leukemia (APL) in mouse APL models. PHRF1 also participates in modulating non-homologous end-joining. However, the role of PHRF1 in mammalian dendrite architecture and synap...

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Autores principales:	Ting-Wei Shih, Li-Jen Lee, Ho-Ching Chang, Hung-Wei Lin, Mau-Sun Chang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3f25b96401d48c68aa70b8efa268aab
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spelling	oai:doaj.org-article:e3f25b96401d48c68aa70b8efa268aab2021-12-02T16:32:12ZAn important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling10.1038/s41598-020-67675-22045-2322https://doaj.org/article/e3f25b96401d48c68aa70b8efa268aab2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67675-2https://doaj.org/toc/2045-2322Abstract PHRF1 is involved in transforming growth factor β (TGF-β) signaling to constrain the formation of acute promyelocytic leukemia (APL) in mouse APL models. PHRF1 also participates in modulating non-homologous end-joining. However, the role of PHRF1 in mammalian dendrite architecture and synaptic plasticity is unclear. Here, we investigated the role of PHRF1 in dendritic formation in the murine hippocampus using Camk2a promoter driven-iCre recombinase to conduct a PHRF1 conditional knockout, namely PHRF1Δ/Δ, in the forebrain region. PHRF1Δ/Δ mice developed normally, but exhibited anxiety-like behaviors and displayed defective spatial memory. Alterations of dendritic complexity in apical and basal dendrites of pyramidal neurons were noticed in PHRF1Δ/Δ mutants. Furthermore, electrical stimulation in the hippocampal CA1 region after the TGF-β1 treatment showed a reduced synaptic plasticity in PHRF1Δ/Δ mice. Immunoblotting analysis indicated that PHRF1 ablation affected the TGF-β signaling. Collectively, our results demonstrate that PHRF1 is important for the dendritic architecture and required for spatial memory formation in the hippocampus.Ting-Wei ShihLi-Jen LeeHo-Ching ChangHung-Wei LinMau-Sun ChangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ting-Wei Shih Li-Jen Lee Ho-Ching Chang Hung-Wei Lin Mau-Sun Chang An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling
description	Abstract PHRF1 is involved in transforming growth factor β (TGF-β) signaling to constrain the formation of acute promyelocytic leukemia (APL) in mouse APL models. PHRF1 also participates in modulating non-homologous end-joining. However, the role of PHRF1 in mammalian dendrite architecture and synaptic plasticity is unclear. Here, we investigated the role of PHRF1 in dendritic formation in the murine hippocampus using Camk2a promoter driven-iCre recombinase to conduct a PHRF1 conditional knockout, namely PHRF1Δ/Δ, in the forebrain region. PHRF1Δ/Δ mice developed normally, but exhibited anxiety-like behaviors and displayed defective spatial memory. Alterations of dendritic complexity in apical and basal dendrites of pyramidal neurons were noticed in PHRF1Δ/Δ mutants. Furthermore, electrical stimulation in the hippocampal CA1 region after the TGF-β1 treatment showed a reduced synaptic plasticity in PHRF1Δ/Δ mice. Immunoblotting analysis indicated that PHRF1 ablation affected the TGF-β signaling. Collectively, our results demonstrate that PHRF1 is important for the dendritic architecture and required for spatial memory formation in the hippocampus.
format	article
author	Ting-Wei Shih Li-Jen Lee Ho-Ching Chang Hung-Wei Lin Mau-Sun Chang
author_facet	Ting-Wei Shih Li-Jen Lee Ho-Ching Chang Hung-Wei Lin Mau-Sun Chang
author_sort	Ting-Wei Shih
title	An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling
title_short	An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling
title_full	An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling
title_fullStr	An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling
title_full_unstemmed	An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling
title_sort	important role of phrf1 in dendritic architecture and memory formation by modulating tgf-β signaling
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e3f25b96401d48c68aa70b8efa268aab
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An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling

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