Genomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>

ABSTRACT In this study, we sequenced and analyzed the genomes of 40 strains, in addition to the already-reported two type strains, of two Crithidia species infecting bumblebees in Alaska and Central Europe and demonstrated that different strains of Crithidia bombi and C. expoeki vary considerably in...

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Autores principales:	Evgeny Gerasimov, Niklaus Zemp, Regula Schmid-Hempel, Paul Schmid-Hempel, Vyacheslav Yurchenko
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Crithidia Trypanosomatidae genomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e3f5dba6d5c342c0a682af8bb2b40989
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spelling	oai:doaj.org-article:e3f5dba6d5c342c0a682af8bb2b409892021-11-15T15:27:32ZGenomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>10.1128/mSphere.00482-192379-5042https://doaj.org/article/e3f5dba6d5c342c0a682af8bb2b409892019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00482-19https://doaj.org/toc/2379-5042ABSTRACT In this study, we sequenced and analyzed the genomes of 40 strains, in addition to the already-reported two type strains, of two Crithidia species infecting bumblebees in Alaska and Central Europe and demonstrated that different strains of Crithidia bombi and C. expoeki vary considerably in terms of single nucleotide polymorphisms and gene copy number. Based on the genomic structure, phylogenetic analyses, and the pattern of copy number variation, we confirmed the status of C. expoeki as a separate species. The Alaskan populations appear to be clearly separated from those of Central Europe. This pattern fits a scenario of rapid host-parasite coevolution, where the selective advantage of a given parasite strain is only temporary. This study provides helpful insights into possible scenarios of selection and diversification of trypanosomatid parasites. IMPORTANCE A group of trypanosomatid flagellates includes several well-studied medically and economically important parasites of vertebrates and plants. Nevertheless, the vast majority of trypanosomatids infect only insects (mostly flies and true bugs) and, because of that, has attracted little research attention in the past. Of several hundred trypanosomatid species, only four can infect bees (honeybees and bumblebees). Because of such scarcity, these parasites are severely understudied. We analyzed whole-genome information for a total of 42 representatives of bee-infecting trypanosomatids collected in Central Europe and Alaska from a population genetics point of view. Our data shed light on the evolution, selection, and diversification in this important group of trypanosomatid parasites.Evgeny GerasimovNiklaus ZempRegula Schmid-HempelPaul Schmid-HempelVyacheslav YurchenkoAmerican Society for MicrobiologyarticleCrithidiaTrypanosomatidaegenomicsMicrobiologyQR1-502ENmSphere, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Crithidia Trypanosomatidae genomics Microbiology QR1-502
spellingShingle	Crithidia Trypanosomatidae genomics Microbiology QR1-502 Evgeny Gerasimov Niklaus Zemp Regula Schmid-Hempel Paul Schmid-Hempel Vyacheslav Yurchenko Genomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>
description	ABSTRACT In this study, we sequenced and analyzed the genomes of 40 strains, in addition to the already-reported two type strains, of two Crithidia species infecting bumblebees in Alaska and Central Europe and demonstrated that different strains of Crithidia bombi and C. expoeki vary considerably in terms of single nucleotide polymorphisms and gene copy number. Based on the genomic structure, phylogenetic analyses, and the pattern of copy number variation, we confirmed the status of C. expoeki as a separate species. The Alaskan populations appear to be clearly separated from those of Central Europe. This pattern fits a scenario of rapid host-parasite coevolution, where the selective advantage of a given parasite strain is only temporary. This study provides helpful insights into possible scenarios of selection and diversification of trypanosomatid parasites. IMPORTANCE A group of trypanosomatid flagellates includes several well-studied medically and economically important parasites of vertebrates and plants. Nevertheless, the vast majority of trypanosomatids infect only insects (mostly flies and true bugs) and, because of that, has attracted little research attention in the past. Of several hundred trypanosomatid species, only four can infect bees (honeybees and bumblebees). Because of such scarcity, these parasites are severely understudied. We analyzed whole-genome information for a total of 42 representatives of bee-infecting trypanosomatids collected in Central Europe and Alaska from a population genetics point of view. Our data shed light on the evolution, selection, and diversification in this important group of trypanosomatid parasites.
format	article
author	Evgeny Gerasimov Niklaus Zemp Regula Schmid-Hempel Paul Schmid-Hempel Vyacheslav Yurchenko
author_facet	Evgeny Gerasimov Niklaus Zemp Regula Schmid-Hempel Paul Schmid-Hempel Vyacheslav Yurchenko
author_sort	Evgeny Gerasimov
title	Genomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>
title_short	Genomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>
title_full	Genomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>
title_fullStr	Genomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>
title_full_unstemmed	Genomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>
title_sort	genomic variation among strains of <italic toggle="yes">crithidia bombi</italic> and <italic toggle="yes">c. expoeki</italic>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/e3f5dba6d5c342c0a682af8bb2b40989
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Genomic Variation among Strains of <italic toggle="yes">Crithidia bombi</italic> and <italic toggle="yes">C. expoeki</italic>

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