Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus

Background. Development of nutrient media ensuring the maximum growth rate of pathogens of dangerous infectious diseases while preserving their biological properties is extremely important. A promising direction in this area seems to be the use of synthetic microbial growth biostimulants.The aim of...

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Autores principales:	S. V. Lukyanova, N. G. Gefan, S. N. Adamovich, E. N. Oborina, N. M. Khaptanova, V. I. Kuznetsov, A. S. Ostyak, V. S. Kosilko, S. V. Balakhonov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2020
Materias:	cultivation microorganism nutrient medium growth stimulant protathrane listeria monocytogenes staphylococcus aureus Science Q
Acceso en línea:	https://doaj.org/article/e3faa87c8daf4f1e926eb775aabd5696
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spelling	oai:doaj.org-article:e3faa87c8daf4f1e926eb775aabd56962021-11-23T06:14:43ZStudy of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus2541-94202587-959610.29413/ABS.2020-5.1.6https://doaj.org/article/e3faa87c8daf4f1e926eb775aabd56962020-03-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2238https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Development of nutrient media ensuring the maximum growth rate of pathogens of dangerous infectious diseases while preserving their biological properties is extremely important. A promising direction in this area seems to be the use of synthetic microbial growth biostimulants.The aim of the work is to study the possibility of improving nutrient media for the cultivation of Listeria and Staphylococcus using a biologically active compound tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanylacetate.Materials and methods. The object of the study was experimental nutrient medium for the cultivation of Listeria used for the culturing of the test strain Listeria monocytogenes 766. As a comparison medium, commercial medium Fraser broth to which agar was added at a concentration of 1.5 %, was used. The test strain Staphylococcus aureus ATCC 6538-P (FDA 209-P) was cultivated on meat-peptone agar with 1% glucose. The compound tris(2-hydroxyethyl) ammonium (4-chlorophenyl)sulfanylacetate at a concentration of 10–4 wt. % was studied as a growth stimulator. A nutrient medium without a stimulant served as a control. The specific activity of nutrient media (germination rate, medium sensitivity, growth rate and stability of the main biological properties of microorganisms) was evaluated by the microbiological method.Results. Studies have shown that the addition of a growth stimulator to nutrient media contributes to the growth of colonies (by 10–50 %) and a decrease in the time of their development. When growth stimulator was added to the nutrient medium for the cultivation of Listeria, the initial growth of colonies of the L. monocytogenes 766 test strain after 12 hours of cultivation and growth of colonies of the test strain S. aureus ATCC 6538-P after 6 hours of cultivation on the meat-peptone agar with 1% glucose was observed.Conclusion. Thus, the addition of a growth biostimulator tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanyl acetate at a concentration of 10–4 wt. % in the nutrient medium accelerates the growth of Listeria and Staphylococcus, allows to reduce the time of issuance of the analysis result in half.S. V. LukyanovaN. G. GefanS. N. AdamovichE. N. OborinaN. M. KhaptanovaV. I. KuznetsovA. S. OstyakV. S. KosilkoS. V. BalakhonovScientific Сentre for Family Health and Human Reproduction Problemsarticlecultivationmicroorganismnutrient mediumgrowth stimulantprotathranelisteria monocytogenesstaphylococcus aureusScienceQRUActa Biomedica Scientifica, Vol 5, Iss 1, Pp 47-53 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	cultivation microorganism nutrient medium growth stimulant protathrane listeria monocytogenes staphylococcus aureus Science Q
spellingShingle	cultivation microorganism nutrient medium growth stimulant protathrane listeria monocytogenes staphylococcus aureus Science Q S. V. Lukyanova N. G. Gefan S. N. Adamovich E. N. Oborina N. M. Khaptanova V. I. Kuznetsov A. S. Ostyak V. S. Kosilko S. V. Balakhonov Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus
description	Background. Development of nutrient media ensuring the maximum growth rate of pathogens of dangerous infectious diseases while preserving their biological properties is extremely important. A promising direction in this area seems to be the use of synthetic microbial growth biostimulants.The aim of the work is to study the possibility of improving nutrient media for the cultivation of Listeria and Staphylococcus using a biologically active compound tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanylacetate.Materials and methods. The object of the study was experimental nutrient medium for the cultivation of Listeria used for the culturing of the test strain Listeria monocytogenes 766. As a comparison medium, commercial medium Fraser broth to which agar was added at a concentration of 1.5 %, was used. The test strain Staphylococcus aureus ATCC 6538-P (FDA 209-P) was cultivated on meat-peptone agar with 1% glucose. The compound tris(2-hydroxyethyl) ammonium (4-chlorophenyl)sulfanylacetate at a concentration of 10–4 wt. % was studied as a growth stimulator. A nutrient medium without a stimulant served as a control. The specific activity of nutrient media (germination rate, medium sensitivity, growth rate and stability of the main biological properties of microorganisms) was evaluated by the microbiological method.Results. Studies have shown that the addition of a growth stimulator to nutrient media contributes to the growth of colonies (by 10–50 %) and a decrease in the time of their development. When growth stimulator was added to the nutrient medium for the cultivation of Listeria, the initial growth of colonies of the L. monocytogenes 766 test strain after 12 hours of cultivation and growth of colonies of the test strain S. aureus ATCC 6538-P after 6 hours of cultivation on the meat-peptone agar with 1% glucose was observed.Conclusion. Thus, the addition of a growth biostimulator tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanyl acetate at a concentration of 10–4 wt. % in the nutrient medium accelerates the growth of Listeria and Staphylococcus, allows to reduce the time of issuance of the analysis result in half.
format	article
author	S. V. Lukyanova N. G. Gefan S. N. Adamovich E. N. Oborina N. M. Khaptanova V. I. Kuznetsov A. S. Ostyak V. S. Kosilko S. V. Balakhonov
author_facet	S. V. Lukyanova N. G. Gefan S. N. Adamovich E. N. Oborina N. M. Khaptanova V. I. Kuznetsov A. S. Ostyak V. S. Kosilko S. V. Balakhonov
author_sort	S. V. Lukyanova
title	Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus
title_short	Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus
title_full	Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus
title_fullStr	Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus
title_full_unstemmed	Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus
title_sort	study of the effect of a biologically active compound tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanylacetate on the growth of listeria monocytogenes and staphylococcus aureus
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2020
url	https://doaj.org/article/e3faa87c8daf4f1e926eb775aabd5696
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Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus

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