Gene Expression Profiling via Multigene Concatemers.

We established a novel method, Gene Expression Profiling via Multigene Concatemers (MgC-GEP), to study multigene expression patterns simultaneously. This method consists of the following steps: (1) cDNA was obtained using specific reverse primers containing an adaptor. (2) During the initial 1-3 cyc...

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Autores principales:	Kai Jin, Xiaoli Zheng, Yuxian Xia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4009fb624484b1f8d207ba36b6c9219
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spelling	oai:doaj.org-article:e4009fb624484b1f8d207ba36b6c92192021-11-18T07:00:20ZGene Expression Profiling via Multigene Concatemers.1932-620310.1371/journal.pone.0015711https://doaj.org/article/e4009fb624484b1f8d207ba36b6c92192011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21267445/?tool=EBIhttps://doaj.org/toc/1932-6203We established a novel method, Gene Expression Profiling via Multigene Concatemers (MgC-GEP), to study multigene expression patterns simultaneously. This method consists of the following steps: (1) cDNA was obtained using specific reverse primers containing an adaptor. (2) During the initial 1-3 cycles of polymerase chain reaction (PCR), the products containing universal adaptors with digestion sites at both termini were amplified using specific forward and reverse primers containing the adaptors. (3) In the subsequent 4-28 cycles, the universal adaptors were used as primers to yield products. (4) The products were digested and ligated to produce concatemers. (5) The concatemers were cloned into the vector and sequenced. Then, the occurrence of each gene tag was determined. To validate MgC-GEP, we analyzed 20 genes in Saccharomyces cerevisiae induced by weak acid using MgC-GEP combined with real-time reverse transcription (RT)-PCR. Compared with the results of real-time RT-PCR and the previous reports of microarray analysis, MgC-GEP can precisely determine the transcript levels of multigenes simultaneously. Importantly, MgC-GEP is a cost effective strategy that can be widely used in most laboratories without specific equipment. MgC-GEP is a potentially powerful tool for multigene expression profiling, particularly for moderate-throughput analysis.Kai JinXiaoli ZhengYuxian XiaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e15711 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Jin Xiaoli Zheng Yuxian Xia Gene Expression Profiling via Multigene Concatemers.
description	We established a novel method, Gene Expression Profiling via Multigene Concatemers (MgC-GEP), to study multigene expression patterns simultaneously. This method consists of the following steps: (1) cDNA was obtained using specific reverse primers containing an adaptor. (2) During the initial 1-3 cycles of polymerase chain reaction (PCR), the products containing universal adaptors with digestion sites at both termini were amplified using specific forward and reverse primers containing the adaptors. (3) In the subsequent 4-28 cycles, the universal adaptors were used as primers to yield products. (4) The products were digested and ligated to produce concatemers. (5) The concatemers were cloned into the vector and sequenced. Then, the occurrence of each gene tag was determined. To validate MgC-GEP, we analyzed 20 genes in Saccharomyces cerevisiae induced by weak acid using MgC-GEP combined with real-time reverse transcription (RT)-PCR. Compared with the results of real-time RT-PCR and the previous reports of microarray analysis, MgC-GEP can precisely determine the transcript levels of multigenes simultaneously. Importantly, MgC-GEP is a cost effective strategy that can be widely used in most laboratories without specific equipment. MgC-GEP is a potentially powerful tool for multigene expression profiling, particularly for moderate-throughput analysis.
format	article
author	Kai Jin Xiaoli Zheng Yuxian Xia
author_facet	Kai Jin Xiaoli Zheng Yuxian Xia
author_sort	Kai Jin
title	Gene Expression Profiling via Multigene Concatemers.
title_short	Gene Expression Profiling via Multigene Concatemers.
title_full	Gene Expression Profiling via Multigene Concatemers.
title_fullStr	Gene Expression Profiling via Multigene Concatemers.
title_full_unstemmed	Gene Expression Profiling via Multigene Concatemers.
title_sort	gene expression profiling via multigene concatemers.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/e4009fb624484b1f8d207ba36b6c9219
work_keys_str_mv	AT kaijin geneexpressionprofilingviamultigeneconcatemers AT xiaolizheng geneexpressionprofilingviamultigeneconcatemers AT yuxianxia geneexpressionprofilingviamultigeneconcatemers
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Gene Expression Profiling via Multigene Concatemers.

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