Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion

Abstract To achieve DNA sequencing with solid-state nanopores, the speed of the DNA in the nanopore must be controlled to obtain sequence-specific signals. In this study, we fabricated a nanopore-sensing system equipped with a DNA motion controller. DNA strands were immobilized on a Si probe, and ap...

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Autores principales:	Rena Akahori, Itaru Yanagi, Yusuke Goto, Kunio Harada, Takahide Yokoi, Ken-ichi Takeda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e40174e1af4f461b9338e84bbeab5af8
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spelling	oai:doaj.org-article:e40174e1af4f461b9338e84bbeab5af82021-12-02T11:52:25ZDiscrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion10.1038/s41598-017-08290-62045-2322https://doaj.org/article/e40174e1af4f461b9338e84bbeab5af82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08290-6https://doaj.org/toc/2045-2322Abstract To achieve DNA sequencing with solid-state nanopores, the speed of the DNA in the nanopore must be controlled to obtain sequence-specific signals. In this study, we fabricated a nanopore-sensing system equipped with a DNA motion controller. DNA strands were immobilized on a Si probe, and approach of this probe to the nanopore vicinity could be controlled using a piezo actuator and stepper motor. The area of the Si probe was larger than the area of the membrane, which meant that the immobilized DNA could enter the nanopore without the need for the probe to scan to determine the location of the nanopore in the membrane. We demonstrated that a single-stranded DNA could be inserted into and removed from a nanopore in our experimental system. The number of different ionic-current levels observed while DNA remained in the nanopore corresponded to the number of different types of homopolymers in the DNA.Rena AkahoriItaru YanagiYusuke GotoKunio HaradaTakahide YokoiKen-ichi TakedaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rena Akahori Itaru Yanagi Yusuke Goto Kunio Harada Takahide Yokoi Ken-ichi Takeda Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion
description	Abstract To achieve DNA sequencing with solid-state nanopores, the speed of the DNA in the nanopore must be controlled to obtain sequence-specific signals. In this study, we fabricated a nanopore-sensing system equipped with a DNA motion controller. DNA strands were immobilized on a Si probe, and approach of this probe to the nanopore vicinity could be controlled using a piezo actuator and stepper motor. The area of the Si probe was larger than the area of the membrane, which meant that the immobilized DNA could enter the nanopore without the need for the probe to scan to determine the location of the nanopore in the membrane. We demonstrated that a single-stranded DNA could be inserted into and removed from a nanopore in our experimental system. The number of different ionic-current levels observed while DNA remained in the nanopore corresponded to the number of different types of homopolymers in the DNA.
format	article
author	Rena Akahori Itaru Yanagi Yusuke Goto Kunio Harada Takahide Yokoi Ken-ichi Takeda
author_facet	Rena Akahori Itaru Yanagi Yusuke Goto Kunio Harada Takahide Yokoi Ken-ichi Takeda
author_sort	Rena Akahori
title	Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion
title_short	Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion
title_full	Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion
title_fullStr	Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion
title_full_unstemmed	Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion
title_sort	discrimination of three types of homopolymers in single-stranded dna with solid-state nanopores through external control of the dna motion
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e40174e1af4f461b9338e84bbeab5af8
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Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion

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