PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease

Abstract Sigmodon hispidus or cotton rat is an excellent animal model for studying human infections of respiratory viruses including respiratory syncytial virus (RSV), which is the leading cause of hospitalization in infants and causes high rates of infection in the elderly and immunocompromised pat...

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Autores principales:	Abenaya Muralidharan, Louise Larocque, Marsha Russell, Marybeth Creskey, Changgui Li, Wangxue Chen, Gary Van Domselaar, Jingxin Cao, Terry Cyr, Michael Rosu-Myles, Lisheng Wang, Xuguang Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e40d9815733c4271aa9e178757463077
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spelling	oai:doaj.org-article:e40d9815733c4271aa9e1787574630772021-12-02T16:08:04ZPD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease10.1038/s41598-019-48225-x2045-2322https://doaj.org/article/e40d9815733c4271aa9e1787574630772019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48225-xhttps://doaj.org/toc/2045-2322Abstract Sigmodon hispidus or cotton rat is an excellent animal model for studying human infections of respiratory viruses including respiratory syncytial virus (RSV), which is the leading cause of hospitalization in infants and causes high rates of infection in the elderly and immunocompromised patient populations. Despite several decades of research, no vaccine has been licensed whereas inactivated vaccines have been shown to induce severe adverse reaction in a clinical trial, with other forms of RSV vaccine also found to induce enhanced disease in preclinical animal studies. While arguably the cotton rat is the best small animal model for evaluation of RSV vaccines and antivirals, many important genes of the immune system remain to be isolated. Programmed cell death-1 (PD-1) plays an integral role in regulating many aspects of immunity by inducing suppressive signals. In this study, we report the isolation of mRNA encoding the cotton rat PD-1 (crPD-1) and characterization of the PD-1 protein. crPD-1 bound to its cognate ligand on dendritic cells and effectively suppressed cytokine secretion. Moreover, using the newly acquired gene sequence, we observed a decreased level of crPD-1 levels in cotton rats with enhanced respiratory disease induced by inactivated RSV vaccine, unraveling a new facet of vaccine-induced disease.Abenaya MuralidharanLouise LarocqueMarsha RussellMarybeth CreskeyChanggui LiWangxue ChenGary Van DomselaarJingxin CaoTerry CyrMichael Rosu-MylesLisheng WangXuguang LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abenaya Muralidharan Louise Larocque Marsha Russell Marybeth Creskey Changgui Li Wangxue Chen Gary Van Domselaar Jingxin Cao Terry Cyr Michael Rosu-Myles Lisheng Wang Xuguang Li PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease
description	Abstract Sigmodon hispidus or cotton rat is an excellent animal model for studying human infections of respiratory viruses including respiratory syncytial virus (RSV), which is the leading cause of hospitalization in infants and causes high rates of infection in the elderly and immunocompromised patient populations. Despite several decades of research, no vaccine has been licensed whereas inactivated vaccines have been shown to induce severe adverse reaction in a clinical trial, with other forms of RSV vaccine also found to induce enhanced disease in preclinical animal studies. While arguably the cotton rat is the best small animal model for evaluation of RSV vaccines and antivirals, many important genes of the immune system remain to be isolated. Programmed cell death-1 (PD-1) plays an integral role in regulating many aspects of immunity by inducing suppressive signals. In this study, we report the isolation of mRNA encoding the cotton rat PD-1 (crPD-1) and characterization of the PD-1 protein. crPD-1 bound to its cognate ligand on dendritic cells and effectively suppressed cytokine secretion. Moreover, using the newly acquired gene sequence, we observed a decreased level of crPD-1 levels in cotton rats with enhanced respiratory disease induced by inactivated RSV vaccine, unraveling a new facet of vaccine-induced disease.
format	article
author	Abenaya Muralidharan Louise Larocque Marsha Russell Marybeth Creskey Changgui Li Wangxue Chen Gary Van Domselaar Jingxin Cao Terry Cyr Michael Rosu-Myles Lisheng Wang Xuguang Li
author_facet	Abenaya Muralidharan Louise Larocque Marsha Russell Marybeth Creskey Changgui Li Wangxue Chen Gary Van Domselaar Jingxin Cao Terry Cyr Michael Rosu-Myles Lisheng Wang Xuguang Li
author_sort	Abenaya Muralidharan
title	PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease
title_short	PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease
title_full	PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease
title_fullStr	PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease
title_full_unstemmed	PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease
title_sort	pd-1 of sigmodon hispidus: gene identification, characterization and preliminary evaluation of expression in inactivated rsv vaccine-induced enhanced respiratory disease
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/e40d9815733c4271aa9e178757463077
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PD-1 of Sigmodon hispidus: Gene identification, characterization and preliminary evaluation of expression in inactivated RSV vaccine-induced enhanced respiratory disease

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