Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand

Abstract Parainfluenza virus type 5 (PIV-5) causes respiratory infection in several animal species and humans. Canine parainfluenza virus type 5 (CPIV-5) causes respiratory disease in domestic dogs worldwide. In this study, we conducted a cross-sectional survey of CPIV-5 in dogs with respiratory sym...

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Autores principales:	Kamonpan Charoenkul, Chanakarn Nasamran, Taveesak Janetanakit, Supassama Chaiyawong, Napawan Bunpapong, Supanat Boonyapisitsopa, Ratanaporn Tangwangvivat, Alongkorn Amonsin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e41d9940982449d8b419d61f2e0ece5c
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spelling	oai:doaj.org-article:e41d9940982449d8b419d61f2e0ece5c2021-12-02T10:54:30ZMolecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand10.1038/s41598-021-83323-92045-2322https://doaj.org/article/e41d9940982449d8b419d61f2e0ece5c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83323-9https://doaj.org/toc/2045-2322Abstract Parainfluenza virus type 5 (PIV-5) causes respiratory infection in several animal species and humans. Canine parainfluenza virus type 5 (CPIV-5) causes respiratory disease in domestic dogs worldwide. In this study, we conducted a cross-sectional survey of CPIV-5 in dogs with respiratory symptoms from small animal hospitals in Thailand from November 2015 to December 2018. Our results showed that 32 out of 571 nasal swab samples (5.6%) were positive for CPIV-5 by RT-PCR specific to the NP gene. To characterize the viruses, three representative CPIV-5 were subjected to whole genome sequencing, and an additional ten CPIV-5 were subjected to HN, F, SH and V/P gene sequencing. Pairwise sequence comparison and phylogenetic analysis showed that Thai CPIV-5 was closely related to the CPIV-5 isolated from China and Korea. In conclusion, this study constitutes a whole genome characterization of CPIV-5 from dogs in Thailand. The surveillance of CPIV-5 should be further investigated at a larger scale to determine the dynamics, distribution and potential zoonotic transmission of CPIV-5.Kamonpan CharoenkulChanakarn NasamranTaveesak JanetanakitSupassama ChaiyawongNapawan BunpapongSupanat BoonyapisitsopaRatanaporn TangwangvivatAlongkorn AmonsinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kamonpan Charoenkul Chanakarn Nasamran Taveesak Janetanakit Supassama Chaiyawong Napawan Bunpapong Supanat Boonyapisitsopa Ratanaporn Tangwangvivat Alongkorn Amonsin Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand
description	Abstract Parainfluenza virus type 5 (PIV-5) causes respiratory infection in several animal species and humans. Canine parainfluenza virus type 5 (CPIV-5) causes respiratory disease in domestic dogs worldwide. In this study, we conducted a cross-sectional survey of CPIV-5 in dogs with respiratory symptoms from small animal hospitals in Thailand from November 2015 to December 2018. Our results showed that 32 out of 571 nasal swab samples (5.6%) were positive for CPIV-5 by RT-PCR specific to the NP gene. To characterize the viruses, three representative CPIV-5 were subjected to whole genome sequencing, and an additional ten CPIV-5 were subjected to HN, F, SH and V/P gene sequencing. Pairwise sequence comparison and phylogenetic analysis showed that Thai CPIV-5 was closely related to the CPIV-5 isolated from China and Korea. In conclusion, this study constitutes a whole genome characterization of CPIV-5 from dogs in Thailand. The surveillance of CPIV-5 should be further investigated at a larger scale to determine the dynamics, distribution and potential zoonotic transmission of CPIV-5.
format	article
author	Kamonpan Charoenkul Chanakarn Nasamran Taveesak Janetanakit Supassama Chaiyawong Napawan Bunpapong Supanat Boonyapisitsopa Ratanaporn Tangwangvivat Alongkorn Amonsin
author_facet	Kamonpan Charoenkul Chanakarn Nasamran Taveesak Janetanakit Supassama Chaiyawong Napawan Bunpapong Supanat Boonyapisitsopa Ratanaporn Tangwangvivat Alongkorn Amonsin
author_sort	Kamonpan Charoenkul
title	Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand
title_short	Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand
title_full	Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand
title_fullStr	Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand
title_full_unstemmed	Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand
title_sort	molecular detection and whole genome characterization of canine parainfluenza type 5 in thailand
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e41d9940982449d8b419d61f2e0ece5c
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_version_	1718396500863614976

Molecular detection and whole genome characterization of Canine Parainfluenza type 5 in Thailand

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