Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment

Zhuo Long Zhou,1 Jing Ma,2 Ming-Hui Tong,1 Barbara Pui Chan,1 Alice Sze Tsai Wong,2 Alfonso Hing Wan Ngan1 1Department of Mechanical Engineering, 2School of Biological Sciences, University of Hong Kong, Hong Kong, People’s Republic of China Abstract: The adhesion and traction behavior of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhou ZL, Ma J, Tong MH, Chan BP, Wong AST, Ngan AHW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	adhesion migration cell-to-cell contact optical trapping traction-force microscopy protein micropillar matrix Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e4200ae9198d490594456b2fa56291c8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e4200ae9198d490594456b2fa56291c8
record_format	dspace
spelling	oai:doaj.org-article:e4200ae9198d490594456b2fa56291c82021-12-02T03:10:57ZNanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment1178-2013https://doaj.org/article/e4200ae9198d490594456b2fa56291c82016-12-01T00:00:00Zhttps://www.dovepress.com/nanomechanical-measurement-of-adhesion-and-migration-of-leukemia-cells-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhuo Long Zhou,1 Jing Ma,2 Ming-Hui Tong,1 Barbara Pui Chan,1 Alice Sze Tsai Wong,2 Alfonso Hing Wan Ngan1 1Department of Mechanical Engineering, 2School of Biological Sciences, University of Hong Kong, Hong Kong, People’s Republic of China Abstract: The adhesion and traction behavior of leukemia cells in their microenvironment is directly linked to their migration, which is a prime issue affecting the release of cancer cells from the bone marrow and hence metastasis. In assessing the effectiveness of phorbol 12-myristate 13-acetate (PMA) treatment, the conventional batch-cell transwell-migration assay may not indicate the intrinsic effect of the treatment on migration, since the treatment may also affect other cellular behavior, such as proliferation or death. In this study, the pN-level adhesion and traction forces between single leukemia cells and their microenvironment were directly measured using optical tweezers and traction-force microscopy. The effects of PMA on K562 and THP1 leukemia cells were studied, and the results showed that PMA treatment significantly increased cell adhesion with extracellular matrix proteins, bone marrow stromal cells, and human fibroblasts. PMA treatment also significantly increased the traction of THP1 cells on bovine serum albumin proteins, although the effect on K562 cells was insignificant. Western blots showed an increased expression of E-cadherin and vimentin proteins after the leukemia cells were treated with PMA. The study suggests that PMA upregulates adhesion and thus suppresses the migration of both K562 and THP1 cells in their microenvironment. The ability of optical tweezers and traction-force microscopy to measure directly pN-level cell–protein or cell–cell contact was also demonstrated. Keywords: adhesion, migration, cell-to-cell contact, optical trapping, traction-force microscopy, protein micropillar matrixZhou ZLMa JTong MHChan BPWong ASTNgan AHWDove Medical Pressarticleadhesionmigrationcell-to-cell contactoptical trappingtraction-force microscopyprotein micropillar matrixMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6533-6545 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	adhesion migration cell-to-cell contact optical trapping traction-force microscopy protein micropillar matrix Medicine (General) R5-920
spellingShingle	adhesion migration cell-to-cell contact optical trapping traction-force microscopy protein micropillar matrix Medicine (General) R5-920 Zhou ZL Ma J Tong MH Chan BP Wong AST Ngan AHW Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
description	Zhuo Long Zhou,1 Jing Ma,2 Ming-Hui Tong,1 Barbara Pui Chan,1 Alice Sze Tsai Wong,2 Alfonso Hing Wan Ngan1 1Department of Mechanical Engineering, 2School of Biological Sciences, University of Hong Kong, Hong Kong, People’s Republic of China Abstract: The adhesion and traction behavior of leukemia cells in their microenvironment is directly linked to their migration, which is a prime issue affecting the release of cancer cells from the bone marrow and hence metastasis. In assessing the effectiveness of phorbol 12-myristate 13-acetate (PMA) treatment, the conventional batch-cell transwell-migration assay may not indicate the intrinsic effect of the treatment on migration, since the treatment may also affect other cellular behavior, such as proliferation or death. In this study, the pN-level adhesion and traction forces between single leukemia cells and their microenvironment were directly measured using optical tweezers and traction-force microscopy. The effects of PMA on K562 and THP1 leukemia cells were studied, and the results showed that PMA treatment significantly increased cell adhesion with extracellular matrix proteins, bone marrow stromal cells, and human fibroblasts. PMA treatment also significantly increased the traction of THP1 cells on bovine serum albumin proteins, although the effect on K562 cells was insignificant. Western blots showed an increased expression of E-cadherin and vimentin proteins after the leukemia cells were treated with PMA. The study suggests that PMA upregulates adhesion and thus suppresses the migration of both K562 and THP1 cells in their microenvironment. The ability of optical tweezers and traction-force microscopy to measure directly pN-level cell–protein or cell–cell contact was also demonstrated. Keywords: adhesion, migration, cell-to-cell contact, optical trapping, traction-force microscopy, protein micropillar matrix
format	article
author	Zhou ZL Ma J Tong MH Chan BP Wong AST Ngan AHW
author_facet	Zhou ZL Ma J Tong MH Chan BP Wong AST Ngan AHW
author_sort	Zhou ZL
title	Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
title_short	Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
title_full	Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
title_fullStr	Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
title_full_unstemmed	Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
title_sort	nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/e4200ae9198d490594456b2fa56291c8
work_keys_str_mv	AT zhouzl nanomechanicalmeasurementofadhesionandmigrationofleukemiacellswithphorbol12myristate13acetatetreatment AT maj nanomechanicalmeasurementofadhesionandmigrationofleukemiacellswithphorbol12myristate13acetatetreatment AT tongmh nanomechanicalmeasurementofadhesionandmigrationofleukemiacellswithphorbol12myristate13acetatetreatment AT chanbp nanomechanicalmeasurementofadhesionandmigrationofleukemiacellswithphorbol12myristate13acetatetreatment AT wongast nanomechanicalmeasurementofadhesionandmigrationofleukemiacellswithphorbol12myristate13acetatetreatment AT nganahw nanomechanicalmeasurementofadhesionandmigrationofleukemiacellswithphorbol12myristate13acetatetreatment
_version_	1718401951244222464

Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment

Ejemplares similares