Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa

Abstract Crimean–Congo haemorrhagic fever (CCHF) is an emerging tick‐borne disease causing severe and fatal haemorrhagic syndrome in humans. Hyalomma spp. ticks are the primary vectors and sheep are important CCHF virus (CCHFV)‐amplifying hosts. In this study, blood samples and ticks collected in Oc...

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Autores principales:	Médiha Khamassi Khbou, Rihab Romdhane, Faten Bouaicha Zaafouri, Mohsen Bouajila, Limam Sassi, Sofia K. Appelberg, Ansgar Schulz, Ali Mirazimi, Martin H. Groschup, Mourad Rekik, M'hammed Benzarti, Mohamed Gharbi
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Crimean–Congo haemorrhagic fever virus ELISA sheep Tunisia virus neutralisation test Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/e421add740134be1a24596e5e95d1024
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spelling	oai:doaj.org-article:e421add740134be1a24596e5e95d10242021-11-19T17:14:25ZPresence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa2053-109510.1002/vms3.597https://doaj.org/article/e421add740134be1a24596e5e95d10242021-11-01T00:00:00Zhttps://doi.org/10.1002/vms3.597https://doaj.org/toc/2053-1095Abstract Crimean–Congo haemorrhagic fever (CCHF) is an emerging tick‐borne disease causing severe and fatal haemorrhagic syndrome in humans. Hyalomma spp. ticks are the primary vectors and sheep are important CCHF virus (CCHFV)‐amplifying hosts. In this study, blood samples and ticks collected in October 2019 from 270 sheep from 15 farms across Tunisia constituted the main research material. Moreover, the sera of the same animals taken at different periods between 2018 and 2019 were also used to obtain comparative results. To investigate the presence of anti‐CCHFV antibodies in sheep, all sera were tested using ELISA. Reactive sera were further characterised by a virus neutralisation test (VNT). Overall, one out of the 270 tested sheep was both ELISA‐ and strongly VNT‐positive to CCHFV. Another two sheep were borderline ELISA‐positive but did not exhibit neutralising antibodies. Ninety‐one ticks were collected from all sampled sheep, of which 34 (37.4%) belonged to Hyalomma spp. This is the first report of anti‐CCHFV antibodies in sheep from Tunisia. Both the results of this study and the recent CCHFV detection in ticks collected from camels in southern Tunisia indicate that further studies are needed to determine the competent tick vector in the country and to characterise the epidemiological cycle of CCHFV.Médiha Khamassi KhbouRihab RomdhaneFaten Bouaicha ZaafouriMohsen BouajilaLimam SassiSofia K. AppelbergAnsgar SchulzAli MirazimiMartin H. GroschupMourad RekikM'hammed BenzartiMohamed GharbiWileyarticleCrimean–Congo haemorrhagic fever virusELISAsheepTunisiavirus neutralisation testVeterinary medicineSF600-1100ENVeterinary Medicine and Science, Vol 7, Iss 6, Pp 2323-2329 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Crimean–Congo haemorrhagic fever virus ELISA sheep Tunisia virus neutralisation test Veterinary medicine SF600-1100
spellingShingle	Crimean–Congo haemorrhagic fever virus ELISA sheep Tunisia virus neutralisation test Veterinary medicine SF600-1100 Médiha Khamassi Khbou Rihab Romdhane Faten Bouaicha Zaafouri Mohsen Bouajila Limam Sassi Sofia K. Appelberg Ansgar Schulz Ali Mirazimi Martin H. Groschup Mourad Rekik M'hammed Benzarti Mohamed Gharbi Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa
description	Abstract Crimean–Congo haemorrhagic fever (CCHF) is an emerging tick‐borne disease causing severe and fatal haemorrhagic syndrome in humans. Hyalomma spp. ticks are the primary vectors and sheep are important CCHF virus (CCHFV)‐amplifying hosts. In this study, blood samples and ticks collected in October 2019 from 270 sheep from 15 farms across Tunisia constituted the main research material. Moreover, the sera of the same animals taken at different periods between 2018 and 2019 were also used to obtain comparative results. To investigate the presence of anti‐CCHFV antibodies in sheep, all sera were tested using ELISA. Reactive sera were further characterised by a virus neutralisation test (VNT). Overall, one out of the 270 tested sheep was both ELISA‐ and strongly VNT‐positive to CCHFV. Another two sheep were borderline ELISA‐positive but did not exhibit neutralising antibodies. Ninety‐one ticks were collected from all sampled sheep, of which 34 (37.4%) belonged to Hyalomma spp. This is the first report of anti‐CCHFV antibodies in sheep from Tunisia. Both the results of this study and the recent CCHFV detection in ticks collected from camels in southern Tunisia indicate that further studies are needed to determine the competent tick vector in the country and to characterise the epidemiological cycle of CCHFV.
format	article
author	Médiha Khamassi Khbou Rihab Romdhane Faten Bouaicha Zaafouri Mohsen Bouajila Limam Sassi Sofia K. Appelberg Ansgar Schulz Ali Mirazimi Martin H. Groschup Mourad Rekik M'hammed Benzarti Mohamed Gharbi
author_facet	Médiha Khamassi Khbou Rihab Romdhane Faten Bouaicha Zaafouri Mohsen Bouajila Limam Sassi Sofia K. Appelberg Ansgar Schulz Ali Mirazimi Martin H. Groschup Mourad Rekik M'hammed Benzarti Mohamed Gharbi
author_sort	Médiha Khamassi Khbou
title	Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa
title_short	Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa
title_full	Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa
title_fullStr	Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa
title_full_unstemmed	Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa
title_sort	presence of antibodies to crimean congo haemorrhagic fever virus in sheep in tunisia, north africa
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/e421add740134be1a24596e5e95d1024
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Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa

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