Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression

Osteosarcoma is a highly aggressive malignant tumor, which most commonly occurs in children and adolescents. This study aims to reveal that hypoxia promotes the invasion of osteosarcoma cells by up-regulating the expression of NUSAP1. The expression of HIF-1α and NUSAP1 was significantly up-regulate...

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Autores principales:	Zhang Ling, Song Jingtao, Xin Xu, Sun Donghong, Huang Huiting, Chen Yang, Zhang Tao, Zhang Yiming
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	osteosarcoma hypoxia hif-1α nusap1 migration invasion Medicine R
Acceso en línea:	https://doaj.org/article/e42794eaa9c94b47bb3a69870d22f8d6
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spelling	oai:doaj.org-article:e42794eaa9c94b47bb3a69870d22f8d62021-12-05T14:10:53ZHypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression2391-546310.1515/med-2020-0180https://doaj.org/article/e42794eaa9c94b47bb3a69870d22f8d62021-07-01T00:00:00Zhttps://doi.org/10.1515/med-2020-0180https://doaj.org/toc/2391-5463Osteosarcoma is a highly aggressive malignant tumor, which most commonly occurs in children and adolescents. This study aims to reveal that hypoxia promotes the invasion of osteosarcoma cells by up-regulating the expression of NUSAP1. The expression of HIF-1α and NUSAP1 was significantly up-regulated in MG63 cells cultured in hypoxia for 6–36 h. Furthermore, hypoxia induced the migration and invasion of MG63 cells and regulated the level of E-cad, N-cad, Vimentin, Snail, Slug, MMP2, and MMP9 proteins. Importantly, knockdown of NUSAP1 inhibited hypoxia-induced cell migration and invasion. In the hypoxia microenvironment, the addition of HIF-1α inhibitor or the transfection of siRNA specifically targeting HIF-1α significantly reduced the expression of HIF-1α and NUSAP1 and markedly inhibited the migration and invasion of MG63 cells under the hypoxia microenvironment. In conclusion, hypoxia induced the expression of NUSAP1 in a HIF-1α-dependent manner, stimulating the migration and invasion of MG63 cells.Zhang LingSong JingtaoXin XuSun DonghongHuang HuitingChen YangZhang TaoZhang YimingDe Gruyterarticleosteosarcomahypoxiahif-1αnusap1migrationinvasionMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1083-1089 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osteosarcoma hypoxia hif-1α nusap1 migration invasion Medicine R
spellingShingle	osteosarcoma hypoxia hif-1α nusap1 migration invasion Medicine R Zhang Ling Song Jingtao Xin Xu Sun Donghong Huang Huiting Chen Yang Zhang Tao Zhang Yiming Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression
description	Osteosarcoma is a highly aggressive malignant tumor, which most commonly occurs in children and adolescents. This study aims to reveal that hypoxia promotes the invasion of osteosarcoma cells by up-regulating the expression of NUSAP1. The expression of HIF-1α and NUSAP1 was significantly up-regulated in MG63 cells cultured in hypoxia for 6–36 h. Furthermore, hypoxia induced the migration and invasion of MG63 cells and regulated the level of E-cad, N-cad, Vimentin, Snail, Slug, MMP2, and MMP9 proteins. Importantly, knockdown of NUSAP1 inhibited hypoxia-induced cell migration and invasion. In the hypoxia microenvironment, the addition of HIF-1α inhibitor or the transfection of siRNA specifically targeting HIF-1α significantly reduced the expression of HIF-1α and NUSAP1 and markedly inhibited the migration and invasion of MG63 cells under the hypoxia microenvironment. In conclusion, hypoxia induced the expression of NUSAP1 in a HIF-1α-dependent manner, stimulating the migration and invasion of MG63 cells.
format	article
author	Zhang Ling Song Jingtao Xin Xu Sun Donghong Huang Huiting Chen Yang Zhang Tao Zhang Yiming
author_facet	Zhang Ling Song Jingtao Xin Xu Sun Donghong Huang Huiting Chen Yang Zhang Tao Zhang Yiming
author_sort	Zhang Ling
title	Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression
title_short	Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression
title_full	Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression
title_fullStr	Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression
title_full_unstemmed	Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression
title_sort	hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the nusap1 expression
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/e42794eaa9c94b47bb3a69870d22f8d6
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Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression

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