Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase

Abstract Staphylococcus aureus represents a serious infectious threat to global public health and a vaccine against S. aureus represents an unmet medical need. We here characterise two S. aureus vaccine candidates, coproporphyrinogen III oxidase (CgoX) and triose phosphate isomerase (TPI), which ful...

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Autores principales:	Alexander Klimka, Sonja Mertins, Anne Kristin Nicolai, Liza Marie Rummler, Paul G. Higgins, Saskia Diana Günther, Bettina Tosetti, Oleg Krut, Martin Krönke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e431207387584fa2afd867fea5da2aca
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spelling	oai:doaj.org-article:e431207387584fa2afd867fea5da2aca2021-12-02T15:23:47ZEpitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase10.1038/s41541-020-00268-22059-0105https://doaj.org/article/e431207387584fa2afd867fea5da2aca2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41541-020-00268-2https://doaj.org/toc/2059-0105Abstract Staphylococcus aureus represents a serious infectious threat to global public health and a vaccine against S. aureus represents an unmet medical need. We here characterise two S. aureus vaccine candidates, coproporphyrinogen III oxidase (CgoX) and triose phosphate isomerase (TPI), which fulfil essential housekeeping functions in heme synthesis and glycolysis, respectively. Immunisation with rCgoX and rTPI elicited protective immunity against S. aureus bacteremia. Two monoclonal antibodies (mAb), CgoX-D3 and TPI-H8, raised against CgoX and TPI, efficiently provided protection against S. aureus infection. MAb-CgoX-D3 recognised a linear epitope spanning 12 amino acids (aa), whereas TPI-H8 recognised a larger discontinuous epitope. The CgoX-D3 epitope conjugated to BSA elicited a strong, protective immune response against S. aureus infection. The CgoX-D3 epitope is highly conserved in clinical S. aureus isolates, indicating its potential wide usability against S. aureus infection. These data suggest that immunofocusing through epitope-based immunisation constitutes a strategy for the development of a S. aureus vaccine with greater efficacy and better safety profile.Alexander KlimkaSonja MertinsAnne Kristin NicolaiLiza Marie RummlerPaul G. HigginsSaskia Diana GüntherBettina TosettiOleg KrutMartin KrönkeNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Alexander Klimka Sonja Mertins Anne Kristin Nicolai Liza Marie Rummler Paul G. Higgins Saskia Diana Günther Bettina Tosetti Oleg Krut Martin Krönke Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase
description	Abstract Staphylococcus aureus represents a serious infectious threat to global public health and a vaccine against S. aureus represents an unmet medical need. We here characterise two S. aureus vaccine candidates, coproporphyrinogen III oxidase (CgoX) and triose phosphate isomerase (TPI), which fulfil essential housekeeping functions in heme synthesis and glycolysis, respectively. Immunisation with rCgoX and rTPI elicited protective immunity against S. aureus bacteremia. Two monoclonal antibodies (mAb), CgoX-D3 and TPI-H8, raised against CgoX and TPI, efficiently provided protection against S. aureus infection. MAb-CgoX-D3 recognised a linear epitope spanning 12 amino acids (aa), whereas TPI-H8 recognised a larger discontinuous epitope. The CgoX-D3 epitope conjugated to BSA elicited a strong, protective immune response against S. aureus infection. The CgoX-D3 epitope is highly conserved in clinical S. aureus isolates, indicating its potential wide usability against S. aureus infection. These data suggest that immunofocusing through epitope-based immunisation constitutes a strategy for the development of a S. aureus vaccine with greater efficacy and better safety profile.
format	article
author	Alexander Klimka Sonja Mertins Anne Kristin Nicolai Liza Marie Rummler Paul G. Higgins Saskia Diana Günther Bettina Tosetti Oleg Krut Martin Krönke
author_facet	Alexander Klimka Sonja Mertins Anne Kristin Nicolai Liza Marie Rummler Paul G. Higgins Saskia Diana Günther Bettina Tosetti Oleg Krut Martin Krönke
author_sort	Alexander Klimka
title	Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase
title_short	Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase
title_full	Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase
title_fullStr	Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase
title_full_unstemmed	Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase
title_sort	epitope-specific immunity against staphylococcus aureus coproporphyrinogen iii oxidase
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e431207387584fa2afd867fea5da2aca
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Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase

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