The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine

Abstract Despite the importance of immunity against neuraminidase (NA), NA content and immunogenicity are neglected in current influenza vaccines. To address this, a recombinant N1/N2 NA vaccine (NAV) was developed. Stability assays were used to determine optimal temperature and buffer conditions fo...

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Autores principales:	Luca T. Giurgea, Jae-Keun Park, Kathie-Anne Walters, Kelsey Scherler, Adriana Cervantes-Medina, Ashley Freeman, Luz Angela Rosas, John C. Kash, Jeffery K. Taubenberger, Matthew J. Memoli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e4321783a19a4370b86945991fe73acd
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spelling	oai:doaj.org-article:e4321783a19a4370b86945991fe73acd2021-12-02T14:17:31ZThe effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine10.1038/s41541-021-00310-x2059-0105https://doaj.org/article/e4321783a19a4370b86945991fe73acd2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00310-xhttps://doaj.org/toc/2059-0105Abstract Despite the importance of immunity against neuraminidase (NA), NA content and immunogenicity are neglected in current influenza vaccines. To address this, a recombinant N1/N2 NA vaccine (NAV) was developed. Stability assays were used to determine optimal temperature and buffer conditions for vaccine storage. The effect of divalent cation-related enhancement of NA stability and activity on N1 and N2 immunogenicity and efficacy against viral challenge was assessed. Differences in activity between N1 and N2 and cation-related activity enhancement did not translate into differences in immunogenicity or efficacy. NAV-vaccinated mice showed robust antibody titers against N1 and N2, and after challenge with influenza A (H1N1) virus, decreased viral titers and decreased antiviral and inflammatory responses by transcriptomic analysis. These findings provide guidance for optimal storage and assessment of NA-based vaccines and confirm the importance of NA in influenza vaccination strategies in attenuating viral replication and limiting inflammatory responses necessary to clear infection.Luca T. GiurgeaJae-Keun ParkKathie-Anne WaltersKelsey ScherlerAdriana Cervantes-MedinaAshley FreemanLuz Angela RosasJohn C. KashJeffery K. TaubenbergerMatthew J. MemoliNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Luca T. Giurgea Jae-Keun Park Kathie-Anne Walters Kelsey Scherler Adriana Cervantes-Medina Ashley Freeman Luz Angela Rosas John C. Kash Jeffery K. Taubenberger Matthew J. Memoli The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine
description	Abstract Despite the importance of immunity against neuraminidase (NA), NA content and immunogenicity are neglected in current influenza vaccines. To address this, a recombinant N1/N2 NA vaccine (NAV) was developed. Stability assays were used to determine optimal temperature and buffer conditions for vaccine storage. The effect of divalent cation-related enhancement of NA stability and activity on N1 and N2 immunogenicity and efficacy against viral challenge was assessed. Differences in activity between N1 and N2 and cation-related activity enhancement did not translate into differences in immunogenicity or efficacy. NAV-vaccinated mice showed robust antibody titers against N1 and N2, and after challenge with influenza A (H1N1) virus, decreased viral titers and decreased antiviral and inflammatory responses by transcriptomic analysis. These findings provide guidance for optimal storage and assessment of NA-based vaccines and confirm the importance of NA in influenza vaccination strategies in attenuating viral replication and limiting inflammatory responses necessary to clear infection.
format	article
author	Luca T. Giurgea Jae-Keun Park Kathie-Anne Walters Kelsey Scherler Adriana Cervantes-Medina Ashley Freeman Luz Angela Rosas John C. Kash Jeffery K. Taubenberger Matthew J. Memoli
author_facet	Luca T. Giurgea Jae-Keun Park Kathie-Anne Walters Kelsey Scherler Adriana Cervantes-Medina Ashley Freeman Luz Angela Rosas John C. Kash Jeffery K. Taubenberger Matthew J. Memoli
author_sort	Luca T. Giurgea
title	The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine
title_short	The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine
title_full	The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine
title_fullStr	The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine
title_full_unstemmed	The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine
title_sort	effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant n1/n2 neuraminidase vaccine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e4321783a19a4370b86945991fe73acd
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The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine

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