Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems

Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems

Zhaoshun Jiang, Cong Sun, Zhaohui Yin, Fang Zhou, Linfu Ge, Ximin Liu, Fansheng KongGeneral Hospital of Ji’nan Command, PLA, Ji'nan, Shandong, ChinaBackground: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized...

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Autores principales: Jiang Z, Sun C, Yin Z, Zhou F, Ge L, Liu X, Kong F
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/e43d288242224842aa6aa375f7cf724f
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spelling oai:doaj.org-article:e43d288242224842aa6aa375f7cf724f2021-12-02T04:23:49ZComparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems1176-91141178-2013https://doaj.org/article/e43d288242224842aa6aa375f7cf724f2012-04-01T00:00:00Zhttp://www.dovepress.com/comparison-of-two-kinds-of-nanomedicine-for-targeted-gene-therapy-prem-a9704https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Zhaoshun Jiang, Cong Sun, Zhaohui Yin, Fang Zhou, Linfu Ge, Ximin Liu, Fansheng KongGeneral Hospital of Ji’nan Command, PLA, Ji'nan, Shandong, ChinaBackground: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel conjugated mannan-containing PEG and L-α-phosphatidylethanolamine (PE).Methods: Premodified and postmodified solid lipid nanoparticles were prepared and the characteristics of the two kinds of vehicles were evaluated. The modified vectors were then administered intravenously to rats and the in vivo targeting behavior of the complexes was investigated in liver macrophages.Results: By carefully formulating the carriers with an optimal ratio of mannan-containing PEG-PE, postmodified vehicles displayed more efficient gene expression in rat Kupffer cells both in vitro and in vivo.Conclusion: Postmodified gene carriers are superior to premodified gene vectors, although the latter is also promising for targeted gene delivery. This discovery could guide our future research.Keywords: gene therapy, nanomedicine, mannan, polyethylene glycol, L-α-phosphatidylethanolamine, ligands, premodification, postmodificationJiang ZSun CYin ZZhou FGe LLiu XKong FDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2019-2031 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Jiang Z
Sun C
Yin Z
Zhou F
Ge L
Liu X
Kong F
Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
description Zhaoshun Jiang, Cong Sun, Zhaohui Yin, Fang Zhou, Linfu Ge, Ximin Liu, Fansheng KongGeneral Hospital of Ji’nan Command, PLA, Ji'nan, Shandong, ChinaBackground: The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel conjugated mannan-containing PEG and L-α-phosphatidylethanolamine (PE).Methods: Premodified and postmodified solid lipid nanoparticles were prepared and the characteristics of the two kinds of vehicles were evaluated. The modified vectors were then administered intravenously to rats and the in vivo targeting behavior of the complexes was investigated in liver macrophages.Results: By carefully formulating the carriers with an optimal ratio of mannan-containing PEG-PE, postmodified vehicles displayed more efficient gene expression in rat Kupffer cells both in vitro and in vivo.Conclusion: Postmodified gene carriers are superior to premodified gene vectors, although the latter is also promising for targeted gene delivery. This discovery could guide our future research.Keywords: gene therapy, nanomedicine, mannan, polyethylene glycol, L-α-phosphatidylethanolamine, ligands, premodification, postmodification
format article
author Jiang Z
Sun C
Yin Z
Zhou F
Ge L
Liu X
Kong F
author_facet Jiang Z
Sun C
Yin Z
Zhou F
Ge L
Liu X
Kong F
author_sort Jiang Z
title Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_short Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_full Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_fullStr Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_full_unstemmed Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
title_sort comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/e43d288242224842aa6aa375f7cf724f
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AT sunc comparisonoftwokindsofnanomedicinefortargetedgenetherapypremodifiedorpostmodifiedgenedeliverysystems
AT yinz comparisonoftwokindsofnanomedicinefortargetedgenetherapypremodifiedorpostmodifiedgenedeliverysystems
AT zhouf comparisonoftwokindsofnanomedicinefortargetedgenetherapypremodifiedorpostmodifiedgenedeliverysystems
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