Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida

High nitrate concentration is a dangerous pollutant in the environment. Immobilization for the optimum denitrifying bacterial strain isolated from collected wastewater samples was suggested for bioremediation of excessive nitrate concentration from aqueous solutions and its denitrification activity...

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Autores principales:	Marwa E. El-Sesy, Sabah S. Ibrahim
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	bioremediation central composite design immobilized bacteria and denitrification nitrate contamination wastewater Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/e44810e4dff64557a891061713eff0c1
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spelling	oai:doaj.org-article:e44810e4dff64557a891061713eff0c12021-11-06T11:09:44ZApplication of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida0273-12231996-973210.2166/wst.2021.190https://doaj.org/article/e44810e4dff64557a891061713eff0c12021-06-01T00:00:00Zhttp://wst.iwaponline.com/content/83/12/2931https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732High nitrate concentration is a dangerous pollutant in the environment. Immobilization for the optimum denitrifying bacterial strain isolated from collected wastewater samples was suggested for bioremediation of excessive nitrate concentration from aqueous solutions and its denitrification activity under different pH, nitrate concentration, bacterial beads, temperature and sodium alginate concentration conditions was explored. The active isolate was identified as Pseudomonas putida MT364822.1 by 16S rRNA analysis. Nitrate bioremediation process was optimized by applying response surface methodology based on central composite design approach. Nitrate uptake was significantly affected by variables of study (P-value <0.05). Maximum removal of nitrate (91.1%) was achieved at pH 7, nitrate concentration 400 mg/L, immobilized bacterial beads 3.0 g/L, temperature 35 °C and sodium alginate concentration 2.5% as optimal variable values. For application, immobilized P. putida MT364822.1 removed 82.2% of nitrate from raw fish farm effluent. Storage and reusability experiments showed that the immobilized strain stronger and more stable than the pure strain. The results suggested that immobilized P. putida MT364822.1 is a highly promising and suitable microorganism for use in the bio-removal of nitrate, and the central composite design was more effective in optimizing variables to achieve the best nitrate removal efficiency. HIGHLIGHTS Bioremediation of the excessive nitrate concentration from aqueous solutions using bacterial isolates from wastewaters.; Immobilization for the best denitrifying bacterial strain isolated during the study.; Applying response surface methodology based on central composite design approach for optimization.; An application using immobilized denitrifying bacteria in nitrate bio-removal from raw fish farm effluent was studied.;Marwa E. El-SesySabah S. IbrahimIWA Publishingarticlebioremediationcentral composite designimmobilized bacteria and denitrificationnitrate contaminationwastewaterEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 12, Pp 2931-2946 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioremediation central composite design immobilized bacteria and denitrification nitrate contamination wastewater Environmental technology. Sanitary engineering TD1-1066
spellingShingle	bioremediation central composite design immobilized bacteria and denitrification nitrate contamination wastewater Environmental technology. Sanitary engineering TD1-1066 Marwa E. El-Sesy Sabah S. Ibrahim Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida
description	High nitrate concentration is a dangerous pollutant in the environment. Immobilization for the optimum denitrifying bacterial strain isolated from collected wastewater samples was suggested for bioremediation of excessive nitrate concentration from aqueous solutions and its denitrification activity under different pH, nitrate concentration, bacterial beads, temperature and sodium alginate concentration conditions was explored. The active isolate was identified as Pseudomonas putida MT364822.1 by 16S rRNA analysis. Nitrate bioremediation process was optimized by applying response surface methodology based on central composite design approach. Nitrate uptake was significantly affected by variables of study (P-value <0.05). Maximum removal of nitrate (91.1%) was achieved at pH 7, nitrate concentration 400 mg/L, immobilized bacterial beads 3.0 g/L, temperature 35 °C and sodium alginate concentration 2.5% as optimal variable values. For application, immobilized P. putida MT364822.1 removed 82.2% of nitrate from raw fish farm effluent. Storage and reusability experiments showed that the immobilized strain stronger and more stable than the pure strain. The results suggested that immobilized P. putida MT364822.1 is a highly promising and suitable microorganism for use in the bio-removal of nitrate, and the central composite design was more effective in optimizing variables to achieve the best nitrate removal efficiency. HIGHLIGHTS Bioremediation of the excessive nitrate concentration from aqueous solutions using bacterial isolates from wastewaters.; Immobilization for the best denitrifying bacterial strain isolated during the study.; Applying response surface methodology based on central composite design approach for optimization.; An application using immobilized denitrifying bacteria in nitrate bio-removal from raw fish farm effluent was studied.;
format	article
author	Marwa E. El-Sesy Sabah S. Ibrahim
author_facet	Marwa E. El-Sesy Sabah S. Ibrahim
author_sort	Marwa E. El-Sesy
title	Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida
title_short	Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida
title_full	Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida
title_fullStr	Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida
title_full_unstemmed	Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida
title_sort	application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized pseudomonas putida
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/e44810e4dff64557a891061713eff0c1
work_keys_str_mv	AT marwaeelsesy applicationofcentralcompositedesignapproachforoptimizationnitrateremovalfromaqueoussolutionbyimmobilizedpseudomonasputida AT sabahsibrahim applicationofcentralcompositedesignapproachforoptimizationnitrateremovalfromaqueoussolutionbyimmobilizedpseudomonasputida
_version_	1718443765317763072

Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida

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