Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy

Beshir Legas Muhammed, Mohammed Hussen Seid, Adere Tarekegne Habte Department of Chemistry, Woldia University, Woldia, EthiopiaCorrespondence: Beshir Legas Muhammed Email legasbeshir@yahoo.comBackground: Coffee is a well-known beverage that is widely used around the world. Despite the wide use of co...

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Autores principales:	Legas Muhammed B, Hussen Seid M, Habte AT
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	caffeine coffee hydrogen peroxide scavenging uv-vis spectroscopy Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/e44d4850e65a4b158b0aa91472f3a34d
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spelling	oai:doaj.org-article:e44d4850e65a4b158b0aa91472f3a34d2021-12-02T14:57:35ZDetermination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy1179-1438https://doaj.org/article/e44d4850e65a4b158b0aa91472f3a34d2021-05-01T00:00:00Zhttps://www.dovepress.com/determination-of-caffeine-and-hydrogen-peroxide-antioxidant-activity-o-peer-reviewed-fulltext-article-CPAAhttps://doaj.org/toc/1179-1438Beshir Legas Muhammed, Mohammed Hussen Seid, Adere Tarekegne Habte Department of Chemistry, Woldia University, Woldia, EthiopiaCorrespondence: Beshir Legas Muhammed Email legasbeshir@yahoo.comBackground: Coffee is a well-known beverage that is widely used around the world. Despite the wide use of coffee in Ethiopia, there is a lack of extensive studies addressing the issues related to the caffeine content and hydrogen peroxide antioxidant activity of varieties of coffee types, particularly in Habru woreda, Ethiopia.Objective: This study aimed to determine the caffeine content and hydrogen peroxide antioxidant activity of raw and roasted coffee beans collected directly from Habru woreda, North Wollo zone of Ethiopia.Methods: The study was conducted in Bohoro, Girana, and Wurgisa kebeles of Habru woreda, Ethiopia, by collecting 500 g of green beans of Arabica coffee without considering their variety. Then, the collected beans were divided into raw and roasted coffee to perform aqueous and dichloromethane extraction of their caffeine content and hydrogen peroxide antioxidant activity using UV-Vis spectrophotometry.Results: The amounts of caffeine in aqueous and dichloromethane extraction were in the range of 124.01− 191.27 ppm and 145.15− 200.09 ppm in raw and roasted coffees, respectively. Using the IC50 value, the hydrogen peroxide scavenging activity of the aqueous phase coffee bean extracts in Bohoro raw, Bohoro roasted, Wurgisa raw, Wurgisa roasted, Girana raw, and Girana roasted coffee were 32.17 ppm, 11.69 ppm, 26.14 ppm, 3.12 ppm, 24.83 ppm, and 11.06 ppm, respectively, while that of ascorbic acid was 6.91 ppm.Conclusion: The study showed that the highest amount of caffeine in both aqueous and dichloromethane solvent extraction was found in Bohoro’s raw and roasted coffee beans. Also, the amounts of caffeine in all coffee bean samples were safe and the antioxidant activity was excellent. In most of the samples, significant variations in the concentration of caffeine in raw and roasted coffee bean samples were observed in the two extraction solvents.Keywords: caffeine, coffee, hydrogen peroxide scavenging, UV-Vis spectroscopyLegas Muhammed BHussen Seid MHabte ATDove Medical Pressarticlecaffeinecoffeehydrogen peroxide scavenginguv-vis spectroscopyTherapeutics. PharmacologyRM1-950ENClinical Pharmacology: Advances and Applications, Vol Volume 13, Pp 101-113 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	caffeine coffee hydrogen peroxide scavenging uv-vis spectroscopy Therapeutics. Pharmacology RM1-950
spellingShingle	caffeine coffee hydrogen peroxide scavenging uv-vis spectroscopy Therapeutics. Pharmacology RM1-950 Legas Muhammed B Hussen Seid M Habte AT Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy
description	Beshir Legas Muhammed, Mohammed Hussen Seid, Adere Tarekegne Habte Department of Chemistry, Woldia University, Woldia, EthiopiaCorrespondence: Beshir Legas Muhammed Email legasbeshir@yahoo.comBackground: Coffee is a well-known beverage that is widely used around the world. Despite the wide use of coffee in Ethiopia, there is a lack of extensive studies addressing the issues related to the caffeine content and hydrogen peroxide antioxidant activity of varieties of coffee types, particularly in Habru woreda, Ethiopia.Objective: This study aimed to determine the caffeine content and hydrogen peroxide antioxidant activity of raw and roasted coffee beans collected directly from Habru woreda, North Wollo zone of Ethiopia.Methods: The study was conducted in Bohoro, Girana, and Wurgisa kebeles of Habru woreda, Ethiopia, by collecting 500 g of green beans of Arabica coffee without considering their variety. Then, the collected beans were divided into raw and roasted coffee to perform aqueous and dichloromethane extraction of their caffeine content and hydrogen peroxide antioxidant activity using UV-Vis spectrophotometry.Results: The amounts of caffeine in aqueous and dichloromethane extraction were in the range of 124.01− 191.27 ppm and 145.15− 200.09 ppm in raw and roasted coffees, respectively. Using the IC50 value, the hydrogen peroxide scavenging activity of the aqueous phase coffee bean extracts in Bohoro raw, Bohoro roasted, Wurgisa raw, Wurgisa roasted, Girana raw, and Girana roasted coffee were 32.17 ppm, 11.69 ppm, 26.14 ppm, 3.12 ppm, 24.83 ppm, and 11.06 ppm, respectively, while that of ascorbic acid was 6.91 ppm.Conclusion: The study showed that the highest amount of caffeine in both aqueous and dichloromethane solvent extraction was found in Bohoro’s raw and roasted coffee beans. Also, the amounts of caffeine in all coffee bean samples were safe and the antioxidant activity was excellent. In most of the samples, significant variations in the concentration of caffeine in raw and roasted coffee bean samples were observed in the two extraction solvents.Keywords: caffeine, coffee, hydrogen peroxide scavenging, UV-Vis spectroscopy
format	article
author	Legas Muhammed B Hussen Seid M Habte AT
author_facet	Legas Muhammed B Hussen Seid M Habte AT
author_sort	Legas Muhammed B
title	Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy
title_short	Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy
title_full	Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy
title_fullStr	Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy
title_full_unstemmed	Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy
title_sort	determination of caffeine and hydrogen peroxide antioxidant activity of raw and roasted coffee beans around habru woreda, ethiopia using uv-vis spectroscopy
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/e44d4850e65a4b158b0aa91472f3a34d
work_keys_str_mv	AT legasmuhammedb determinationofcaffeineandhydrogenperoxideantioxidantactivityofrawandroastedcoffeebeansaroundhabruworedaethiopiausinguvvisspectroscopy AT hussenseidm determinationofcaffeineandhydrogenperoxideantioxidantactivityofrawandroastedcoffeebeansaroundhabruworedaethiopiausinguvvisspectroscopy AT habteat determinationofcaffeineandhydrogenperoxideantioxidantactivityofrawandroastedcoffeebeansaroundhabruworedaethiopiausinguvvisspectroscopy
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Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy

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