The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer

Björn Logi Isfoss,1–3 Bo Holmqvist,1,4 Helena Jernström,1 Per Alm,1 Håkan Olsson1,5 1Department of Oncology and Pathology, Department of Clinical Sciences Lund, Lund University, 2Department of Pathology, Skane University Hospital, Lund, Sweden; 3Department of Pa...

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Autores principales:	Isfoss BL, Holmqvist B, Jernström H, Alm P, Olsson H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	mammary glands human neoplasms BRCA1 gene stem cells aldehyde dehydrogenase Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e45523b20cb84d858ca799fb287f7418
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spelling	oai:doaj.org-article:e45523b20cb84d858ca799fb287f74182021-12-02T02:31:42ZThe absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer1179-1314https://doaj.org/article/e45523b20cb84d858ca799fb287f74182016-05-01T00:00:00Zhttps://www.dovepress.com/the-absence-of-aldehyde-dehydrogenase-1-a1-positive-cells-in-benign-ma-peer-reviewed-article-BCTThttps://doaj.org/toc/1179-1314Björn Logi Isfoss,1–3 Bo Holmqvist,1,4 Helena Jernström,1 Per Alm,1 Håkan Olsson1,5 1Department of Oncology and Pathology, Department of Clinical Sciences Lund, Lund University, 2Department of Pathology, Skane University Hospital, Lund, Sweden; 3Department of Pathology, Telemark Hospital, Skien, Norway; 4ImaGene-iT AB, Medicon Village, 5Division of Cancer Epidemiology, Department of Clinical Sciences, Lund University, Lund, Sweden Abstract: In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (–). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were CD44– and CD24– were absent in the breast stroma of a significantly larger number of nulliparous than parous women. A less common morphological type of ALDH1-positive cells in stroma was round or oval in shape, and such cells that were CD44+ and CD24– were absent in a significant number of women with a family history of breast cancer. The CD44+/CD24– immunophenotype is consistent with stem cells, and the round/oval morphology suggests mesenchymal cells. This study demonstrates that there are two morphologically distinct types of ALDH1-positive cells in histologically benign mammary stroma, and the absence of these cells is correlated with clinical risk factors for breast cancer in premenopausal women. Keywords: mammary glands, human, neoplasms, BRCA1 gene, stem cells, immunohistochemistryIsfoss BLHolmqvist BJernström HAlm POlsson HDove Medical Pressarticlemammary glandshumanneoplasmsBRCA1 genestem cellsaldehyde dehydrogenaseNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol 2016, Iss Issue 1, Pp 117-124 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	mammary glands human neoplasms BRCA1 gene stem cells aldehyde dehydrogenase Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	mammary glands human neoplasms BRCA1 gene stem cells aldehyde dehydrogenase Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Isfoss BL Holmqvist B Jernström H Alm P Olsson H The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
description	Björn Logi Isfoss,1–3 Bo Holmqvist,1,4 Helena Jernström,1 Per Alm,1 Håkan Olsson1,5 1Department of Oncology and Pathology, Department of Clinical Sciences Lund, Lund University, 2Department of Pathology, Skane University Hospital, Lund, Sweden; 3Department of Pathology, Telemark Hospital, Skien, Norway; 4ImaGene-iT AB, Medicon Village, 5Division of Cancer Epidemiology, Department of Clinical Sciences, Lund University, Lund, Sweden Abstract: In this study, aldehyde dehydrogenase 1 (ALDH1)-expressing cells in stroma of histologically normal breast tissue from premenopausal women were investigated in situ regarding cellular morphology, cell distribution, and relation to the additional stem cell markers, CD44 (+) and CD24 (–). These results were correlated with hormonal and genetic risk factors for breast cancer. Triple immunofluorescence labeling was performed on tissues from premenopausal women with a family history of breast cancer, and breast reduction specimens from premenopausal women with no family history of breast cancer were used as a control group. The majority of ALDH1-immunoreactive cells in stroma were spindle-shaped or polygonal, and such cells that were CD44– and CD24– were absent in the breast stroma of a significantly larger number of nulliparous than parous women. A less common morphological type of ALDH1-positive cells in stroma was round or oval in shape, and such cells that were CD44+ and CD24– were absent in a significant number of women with a family history of breast cancer. The CD44+/CD24– immunophenotype is consistent with stem cells, and the round/oval morphology suggests mesenchymal cells. This study demonstrates that there are two morphologically distinct types of ALDH1-positive cells in histologically benign mammary stroma, and the absence of these cells is correlated with clinical risk factors for breast cancer in premenopausal women. Keywords: mammary glands, human, neoplasms, BRCA1 gene, stem cells, immunohistochemistry
format	article
author	Isfoss BL Holmqvist B Jernström H Alm P Olsson H
author_facet	Isfoss BL Holmqvist B Jernström H Alm P Olsson H
author_sort	Isfoss BL
title	The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
title_short	The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
title_full	The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
title_fullStr	The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
title_full_unstemmed	The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
title_sort	absence of aldehyde dehydrogenase 1 a1-positive cells in benign mammary stroma is associated with risk factors for breast cancer
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/e45523b20cb84d858ca799fb287f7418
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The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer

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