Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo

Tian Zhou,1 Baiyan Sui,1 Xiumei Mo,2,* Jiao Sun1,* 1Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua...

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Autores principales:	Zhou T, Sui B, Mo X, Sun J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	electrospun collagen/bioactive glass nanofibers antibacterial activity skin regeneration angiogenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e4588d894c4448879bee95dc79795b8e
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spelling	oai:doaj.org-article:e4588d894c4448879bee95dc79795b8e2021-12-02T05:09:44ZMultifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo1178-2013https://doaj.org/article/e4588d894c4448879bee95dc79795b8e2017-05-01T00:00:00Zhttps://www.dovepress.com/multifunctional-and-biomimetic-fish-collagenbioactive-glass-nanofibers-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tian Zhou,1 Baiyan Sui,1 Xiumei Mo,2,* Jiao Sun1,* 1Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: The development of skin wound dressings with excellent properties has always been an important challenge in the field of biomedicine. In this study, biomimetic electrospun fish collagen/bioactive glass (Col/BG) nanofibers were prepared. Their structure, tensile strength, antibacterial activity and biological effects on human keratinocytes, human dermal fibroblasts and human vascular endothelial cells were investigated. Furthermore, the Sprague Dawley rat skin defect model was used to validate their effect on wound healing. The results showed that compared with pure fish collagen nanofibers, the tensile strength of the Col/BG nanofibers increased to 21.87±0.21 Mpa, with a certain degree of antibacterial activity against Staphylococcus aureus. It was also found that the Col/BG nanofibers promoted the adhesion, proliferation and migration of human keratinocytes. Col/BG nanofibers induced the secretion of type one collagen and vascular endothelial growth factor by human dermal fibroblasts, which further stimulated the proliferation of human vascular endothelial cells. Animal experimentation indicated that the Col/BG nanofibers could accelerate rat skin wound healing. This study developed a type of multifunctional and biomimetic fish Col/BG nanofibers, which had the ability to induce skin regeneration with adequate tensile strength and antibacterial activity. The Col/BG nanofibers are also easily available and inexpensive, providing the possibility for using as a functional skin wound dressing. Keywords: electrospun, collagen/bioactive glass nanofibers, antibacterial activity, skin regeneration, angiogenesisZhou TSui BMo XSun JDove Medical Pressarticleelectrospuncollagen/bioactive glass nanofibersantibacterial activityskin regenerationangiogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3495-3507 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrospun collagen/bioactive glass nanofibers antibacterial activity skin regeneration angiogenesis Medicine (General) R5-920
spellingShingle	electrospun collagen/bioactive glass nanofibers antibacterial activity skin regeneration angiogenesis Medicine (General) R5-920 Zhou T Sui B Mo X Sun J Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo
description	Tian Zhou,1 Baiyan Sui,1 Xiumei Mo,2,* Jiao Sun1,* 1Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: The development of skin wound dressings with excellent properties has always been an important challenge in the field of biomedicine. In this study, biomimetic electrospun fish collagen/bioactive glass (Col/BG) nanofibers were prepared. Their structure, tensile strength, antibacterial activity and biological effects on human keratinocytes, human dermal fibroblasts and human vascular endothelial cells were investigated. Furthermore, the Sprague Dawley rat skin defect model was used to validate their effect on wound healing. The results showed that compared with pure fish collagen nanofibers, the tensile strength of the Col/BG nanofibers increased to 21.87±0.21 Mpa, with a certain degree of antibacterial activity against Staphylococcus aureus. It was also found that the Col/BG nanofibers promoted the adhesion, proliferation and migration of human keratinocytes. Col/BG nanofibers induced the secretion of type one collagen and vascular endothelial growth factor by human dermal fibroblasts, which further stimulated the proliferation of human vascular endothelial cells. Animal experimentation indicated that the Col/BG nanofibers could accelerate rat skin wound healing. This study developed a type of multifunctional and biomimetic fish Col/BG nanofibers, which had the ability to induce skin regeneration with adequate tensile strength and antibacterial activity. The Col/BG nanofibers are also easily available and inexpensive, providing the possibility for using as a functional skin wound dressing. Keywords: electrospun, collagen/bioactive glass nanofibers, antibacterial activity, skin regeneration, angiogenesis
format	article
author	Zhou T Sui B Mo X Sun J
author_facet	Zhou T Sui B Mo X Sun J
author_sort	Zhou T
title	Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo
title_short	Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo
title_full	Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo
title_fullStr	Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo
title_full_unstemmed	Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo
title_sort	multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/e4588d894c4448879bee95dc79795b8e
work_keys_str_mv	AT zhout multifunctionalandbiomimeticfishcollagenbioactiveglassnanofibersfabricationantibacterialactivityandinducingskinregenerationinvitroandinvivo AT suib multifunctionalandbiomimeticfishcollagenbioactiveglassnanofibersfabricationantibacterialactivityandinducingskinregenerationinvitroandinvivo AT mox multifunctionalandbiomimeticfishcollagenbioactiveglassnanofibersfabricationantibacterialactivityandinducingskinregenerationinvitroandinvivo AT sunj multifunctionalandbiomimeticfishcollagenbioactiveglassnanofibersfabricationantibacterialactivityandinducingskinregenerationinvitroandinvivo
_version_	1718400566397239296

Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo

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