Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis

Abstract Plant-specific WRKY transcription factors play important roles in regulating the expression of defense-responsive genes against pathogen attack. A multiple stress-responsive WRKY gene, ZmWRKY65, was identified in maize by screening salicylic acid (SA)-induced de novo transcriptomic sequence...

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Autores principales:	Tong Huo, Chang-Tao Wang, Tai-Fei Yu, Da-Ming Wang, Meng Li, Dan Zhao, Xiu-Ting Li, Jin-Dong Fu, Zhao-Shi Xu, Xin-Yuan Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e459e5f89c8e4134b61577937e554807
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spelling	oai:doaj.org-article:e459e5f89c8e4134b61577937e5548072021-12-02T10:54:23ZOverexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis10.1038/s41598-021-83440-52045-2322https://doaj.org/article/e459e5f89c8e4134b61577937e5548072021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83440-5https://doaj.org/toc/2045-2322Abstract Plant-specific WRKY transcription factors play important roles in regulating the expression of defense-responsive genes against pathogen attack. A multiple stress-responsive WRKY gene, ZmWRKY65, was identified in maize by screening salicylic acid (SA)-induced de novo transcriptomic sequences. The ZmWRKY65 protein was localized in the nucleus of mesophyll protoplasts. The analysis of the ZmWRKY65 promoter sequence indicated that it contains several stress-related transcriptional regulatory elements. Many environmental factors affecting the transcription of ZmWRKY65 gene, such as drought, salinity, high temperature and low temperature stress. Moreover, the transcription of ZmWRKY65 gene was also affected by the induction of defense related plant hormones such as SA and exogenous ABA. The results of seed germination and stomatal aperture assays indicated that transgenic Arabidopsis plants exhibit enhanced sensitivity to ABA and high concentrations of SA. Overexpression of ZmWRKY65 improved tolerance to both pathogen attack and abiotic stress in transgenic Arabidopsis plants and activated several stress-related genes such as RD29A, ERD10, and STZ as well as pathogenesis-related (PR) genes such as PR1, PR2 and PR5; these genes are involved in resistance to abiotic and biotic stresses in Arabidopsis. Together, this evidence implies that the ZmWRKY65 gene is involved in multiple stress signal transduction pathways.Tong HuoChang-Tao WangTai-Fei YuDa-Ming WangMeng LiDan ZhaoXiu-Ting LiJin-Dong FuZhao-Shi XuXin-Yuan SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tong Huo Chang-Tao Wang Tai-Fei Yu Da-Ming Wang Meng Li Dan Zhao Xiu-Ting Li Jin-Dong Fu Zhao-Shi Xu Xin-Yuan Song Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis
description	Abstract Plant-specific WRKY transcription factors play important roles in regulating the expression of defense-responsive genes against pathogen attack. A multiple stress-responsive WRKY gene, ZmWRKY65, was identified in maize by screening salicylic acid (SA)-induced de novo transcriptomic sequences. The ZmWRKY65 protein was localized in the nucleus of mesophyll protoplasts. The analysis of the ZmWRKY65 promoter sequence indicated that it contains several stress-related transcriptional regulatory elements. Many environmental factors affecting the transcription of ZmWRKY65 gene, such as drought, salinity, high temperature and low temperature stress. Moreover, the transcription of ZmWRKY65 gene was also affected by the induction of defense related plant hormones such as SA and exogenous ABA. The results of seed germination and stomatal aperture assays indicated that transgenic Arabidopsis plants exhibit enhanced sensitivity to ABA and high concentrations of SA. Overexpression of ZmWRKY65 improved tolerance to both pathogen attack and abiotic stress in transgenic Arabidopsis plants and activated several stress-related genes such as RD29A, ERD10, and STZ as well as pathogenesis-related (PR) genes such as PR1, PR2 and PR5; these genes are involved in resistance to abiotic and biotic stresses in Arabidopsis. Together, this evidence implies that the ZmWRKY65 gene is involved in multiple stress signal transduction pathways.
format	article
author	Tong Huo Chang-Tao Wang Tai-Fei Yu Da-Ming Wang Meng Li Dan Zhao Xiu-Ting Li Jin-Dong Fu Zhao-Shi Xu Xin-Yuan Song
author_facet	Tong Huo Chang-Tao Wang Tai-Fei Yu Da-Ming Wang Meng Li Dan Zhao Xiu-Ting Li Jin-Dong Fu Zhao-Shi Xu Xin-Yuan Song
author_sort	Tong Huo
title	Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis
title_short	Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis
title_full	Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis
title_fullStr	Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis
title_full_unstemmed	Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis
title_sort	overexpression of zmwrky65 transcription factor from maize confers stress resistances in transgenic arabidopsis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e459e5f89c8e4134b61577937e554807
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Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis

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