Interaction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity

Zinc oxide (ZnO) nanoparticles (NPs) are used as zinc supplements due to the nutritional value of Zn. The toxicity of ZnO NPs in the food industry is required to be elucidated because they have large surface area and high reactivity compared with bulk-sized materials and have potentials to interact...

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Autores principales:	Eun-Been Jung, Jin Yu, Soo-Jin Choi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	zinc oxide food protein interactions cytotoxicity cellular uptake intestinal transport Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e45ddbf8a4004aebba2422318d7bd62a
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spelling	oai:doaj.org-article:e45ddbf8a4004aebba2422318d7bd62a2021-11-25T18:30:52ZInteraction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity10.3390/nano111129222079-4991https://doaj.org/article/e45ddbf8a4004aebba2422318d7bd62a2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2922https://doaj.org/toc/2079-4991Zinc oxide (ZnO) nanoparticles (NPs) are used as zinc supplements due to the nutritional value of Zn. The toxicity of ZnO NPs in the food industry is required to be elucidated because they have large surface area and high reactivity compared with bulk-sized materials and have potentials to interact with food matrices, which may lead to different biological responses. In this study, interactions between ZnO NPs and food proteins (albumin, casein, and zein) were evaluated by measuring changes in physicochemical property, fluorescence quenching ratios, and structural protein stability compared with ZnO interaction with glucose, the most interacted saccharide in our previous report. The interaction effects on cytotoxicity, cellular uptake, intestinal transport, toxicokinetics, and acute oral toxicity were also investigated. The results demonstrate that interaction between ZnO and albumin reduced hydrodynamic diameters, but increased cytotoxicity, cellular uptake, and intestinal transport in a similar manner to ZnO interaction with glucose, without affecting primary structural protein stability and toxicokinetic behaviors. Hematological, serum biochemical, and histopathological analysis reveal no toxicological findings after orally administered ZnO NPs interacted with albumin or glucose in rats for 14 consecutive days, suggesting their low oral toxicity. In conclusion, the interactions between ZnO NPs and food proteins modulate in vitro biological responses, but do not affect in vivo acute oral toxicity. Further study is required to ascertain the interaction effects on chronic oral toxicity.Eun-Been JungJin YuSoo-Jin ChoiMDPI AGarticlezinc oxidefood proteininteractionscytotoxicitycellular uptakeintestinal transportChemistryQD1-999ENNanomaterials, Vol 11, Iss 2922, p 2922 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zinc oxide food protein interactions cytotoxicity cellular uptake intestinal transport Chemistry QD1-999
spellingShingle	zinc oxide food protein interactions cytotoxicity cellular uptake intestinal transport Chemistry QD1-999 Eun-Been Jung Jin Yu Soo-Jin Choi Interaction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity
description	Zinc oxide (ZnO) nanoparticles (NPs) are used as zinc supplements due to the nutritional value of Zn. The toxicity of ZnO NPs in the food industry is required to be elucidated because they have large surface area and high reactivity compared with bulk-sized materials and have potentials to interact with food matrices, which may lead to different biological responses. In this study, interactions between ZnO NPs and food proteins (albumin, casein, and zein) were evaluated by measuring changes in physicochemical property, fluorescence quenching ratios, and structural protein stability compared with ZnO interaction with glucose, the most interacted saccharide in our previous report. The interaction effects on cytotoxicity, cellular uptake, intestinal transport, toxicokinetics, and acute oral toxicity were also investigated. The results demonstrate that interaction between ZnO and albumin reduced hydrodynamic diameters, but increased cytotoxicity, cellular uptake, and intestinal transport in a similar manner to ZnO interaction with glucose, without affecting primary structural protein stability and toxicokinetic behaviors. Hematological, serum biochemical, and histopathological analysis reveal no toxicological findings after orally administered ZnO NPs interacted with albumin or glucose in rats for 14 consecutive days, suggesting their low oral toxicity. In conclusion, the interactions between ZnO NPs and food proteins modulate in vitro biological responses, but do not affect in vivo acute oral toxicity. Further study is required to ascertain the interaction effects on chronic oral toxicity.
format	article
author	Eun-Been Jung Jin Yu Soo-Jin Choi
author_facet	Eun-Been Jung Jin Yu Soo-Jin Choi
author_sort	Eun-Been Jung
title	Interaction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity
title_short	Interaction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity
title_full	Interaction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity
title_fullStr	Interaction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity
title_full_unstemmed	Interaction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity
title_sort	interaction between zno nanoparticles and albumin and its effect on cytotoxicity, cellular uptake, intestinal transport, toxicokinetics, and acute oral toxicity
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e45ddbf8a4004aebba2422318d7bd62a
work_keys_str_mv	AT eunbeenjung interactionbetweenznonanoparticlesandalbuminanditseffectoncytotoxicitycellularuptakeintestinaltransporttoxicokineticsandacuteoraltoxicity AT jinyu interactionbetweenznonanoparticlesandalbuminanditseffectoncytotoxicitycellularuptakeintestinaltransporttoxicokineticsandacuteoraltoxicity AT soojinchoi interactionbetweenznonanoparticlesandalbuminanditseffectoncytotoxicitycellularuptakeintestinaltransporttoxicokineticsandacuteoraltoxicity
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Interaction between ZnO Nanoparticles and Albumin and Its Effect on Cytotoxicity, Cellular Uptake, Intestinal Transport, Toxicokinetics, and Acute Oral Toxicity

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