Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>

Small non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in minimal medium. Here, by applying a high-reso...

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Autores principales:	Xian Ju, Xingxing Fang, Yunzhu Xiao, Bingyu Li, Ruoping Shi, Chaoliang Wei, Conghui You
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Escherichia coli</i> small RNA GcvB oxidative stress OxyR Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/e4654f4491fe4dd3b94510491b93957b
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spelling	oai:doaj.org-article:e4654f4491fe4dd3b94510491b93957b2021-11-25T16:28:14ZSmall RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>10.3390/antiox101117742076-3921https://doaj.org/article/e4654f4491fe4dd3b94510491b93957b2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1774https://doaj.org/toc/2076-3921Small non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in minimal medium. Here, by applying a high-resolution RNA-seq assay, we compared the transcriptomes of a wild-type <i>Escherichia coli</i> K-12 strain and its <i>gcvB</i> deletion derivative grown in minimal medium and identified putative targets responding to GcvB, including <i>flu</i>, a determinant gene of auto-aggregation. The following molecular studies and the enhanced auto-aggregation ability of the <i>gcvB</i> knockout strain further substantiated the induced expression of these genes. Intriguingly, the reduced expression of OxyR (the oxidative stress regulator) in the <i>gcvB</i> knockout strain was identified to account for the increased expression of <i>flu</i>. Additionally, GcvB was characterized to up-regulate the expression of OxyR at the translational level. Accordingly, compared to the wild type, the GcvB deletion strain was more sensitive to oxidative stress and lost some its ability to eliminate endogenous reactive oxygen species. Taken together, we reveal that GcvB regulates oxidative stress response by up-regulating OxyR expression. Our findings provide an insight into the diversity of GcvB regulation and add an additional layer to the regulation of OxyR.Xian JuXingxing FangYunzhu XiaoBingyu LiRuoping ShiChaoliang WeiConghui YouMDPI AGarticle<i>Escherichia coli</i>small RNAGcvBoxidative stressOxyRTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1774, p 1774 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Escherichia coli</i> small RNA GcvB oxidative stress OxyR Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Escherichia coli</i> small RNA GcvB oxidative stress OxyR Therapeutics. Pharmacology RM1-950 Xian Ju Xingxing Fang Yunzhu Xiao Bingyu Li Ruoping Shi Chaoliang Wei Conghui You Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>
description	Small non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in minimal medium. Here, by applying a high-resolution RNA-seq assay, we compared the transcriptomes of a wild-type <i>Escherichia coli</i> K-12 strain and its <i>gcvB</i> deletion derivative grown in minimal medium and identified putative targets responding to GcvB, including <i>flu</i>, a determinant gene of auto-aggregation. The following molecular studies and the enhanced auto-aggregation ability of the <i>gcvB</i> knockout strain further substantiated the induced expression of these genes. Intriguingly, the reduced expression of OxyR (the oxidative stress regulator) in the <i>gcvB</i> knockout strain was identified to account for the increased expression of <i>flu</i>. Additionally, GcvB was characterized to up-regulate the expression of OxyR at the translational level. Accordingly, compared to the wild type, the GcvB deletion strain was more sensitive to oxidative stress and lost some its ability to eliminate endogenous reactive oxygen species. Taken together, we reveal that GcvB regulates oxidative stress response by up-regulating OxyR expression. Our findings provide an insight into the diversity of GcvB regulation and add an additional layer to the regulation of OxyR.
format	article
author	Xian Ju Xingxing Fang Yunzhu Xiao Bingyu Li Ruoping Shi Chaoliang Wei Conghui You
author_facet	Xian Ju Xingxing Fang Yunzhu Xiao Bingyu Li Ruoping Shi Chaoliang Wei Conghui You
author_sort	Xian Ju
title	Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>
title_short	Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>
title_full	Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>
title_fullStr	Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>
title_full_unstemmed	Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>
title_sort	small rna gcvb regulates oxidative stress response of <i>escherichia coli</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e4654f4491fe4dd3b94510491b93957b
work_keys_str_mv	AT xianju smallrnagcvbregulatesoxidativestressresponseofiescherichiacolii AT xingxingfang smallrnagcvbregulatesoxidativestressresponseofiescherichiacolii AT yunzhuxiao smallrnagcvbregulatesoxidativestressresponseofiescherichiacolii AT bingyuli smallrnagcvbregulatesoxidativestressresponseofiescherichiacolii AT ruopingshi smallrnagcvbregulatesoxidativestressresponseofiescherichiacolii AT chaoliangwei smallrnagcvbregulatesoxidativestressresponseofiescherichiacolii AT conghuiyou smallrnagcvbregulatesoxidativestressresponseofiescherichiacolii
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Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>

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