Multilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).

The bacterium Pseudomonas anguilliseptica has in recent years emerged as a serious threat to production of lumpfish in Norway. Little is known about the population structure of this bacterium despite its association with disease in a wide range of different fish species throughout the world. The phy...

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Autores principales:	Even Bysveen Mjølnerød, Hanne Katrine Nilsen, Snore Gulla, Andreas Riborg, Kirsten Liland Bottolfsen, Tom Wiklund, Debes Christiansen, Jesús Ángel López Romalde, Felix Scholz, Duncan John Colquhoun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e46c0c637e034186a9753e1f5629b3f6
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spelling	oai:doaj.org-article:e46c0c637e034186a9753e1f5629b3f62021-12-02T20:16:19ZMultilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).1932-620310.1371/journal.pone.0259725https://doaj.org/article/e46c0c637e034186a9753e1f5629b3f62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259725https://doaj.org/toc/1932-6203The bacterium Pseudomonas anguilliseptica has in recent years emerged as a serious threat to production of lumpfish in Norway. Little is known about the population structure of this bacterium despite its association with disease in a wide range of different fish species throughout the world. The phylogenetic relationships between 53 isolates, primarily derived from diseased lumpfish, but including a number of reference strains from diverse geographical origins and fish species, were reconstructed by Multi-Locus Sequence Analysis (MLSA) using nine housekeeping genes (rpoB, atpD, gyrB, rpoD, ileS, aroE, carA, glnS and recA). MLSA revealed a high degree of relatedness between the studied isolates, altough the seven genotypes identified formed three main phylogenetic lineages. While four genotypes were identified amongst Norwegian lumpfish isolates, a single genotype dominated, irrespective of geographic origin. This suggests the existence of a dominant genotype associated with disease in production of lumpfish in Norwegian aquaculture. Elucidation of the population structure of the bacterium has provided valuable information for potential future vaccine development.Even Bysveen MjølnerødHanne Katrine NilsenSnore GullaAndreas RiborgKirsten Liland BottolfsenTom WiklundDebes ChristiansenJesús Ángel López RomaldeFelix ScholzDuncan John ColquhounPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259725 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Even Bysveen Mjølnerød Hanne Katrine Nilsen Snore Gulla Andreas Riborg Kirsten Liland Bottolfsen Tom Wiklund Debes Christiansen Jesús Ángel López Romalde Felix Scholz Duncan John Colquhoun Multilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).
description	The bacterium Pseudomonas anguilliseptica has in recent years emerged as a serious threat to production of lumpfish in Norway. Little is known about the population structure of this bacterium despite its association with disease in a wide range of different fish species throughout the world. The phylogenetic relationships between 53 isolates, primarily derived from diseased lumpfish, but including a number of reference strains from diverse geographical origins and fish species, were reconstructed by Multi-Locus Sequence Analysis (MLSA) using nine housekeeping genes (rpoB, atpD, gyrB, rpoD, ileS, aroE, carA, glnS and recA). MLSA revealed a high degree of relatedness between the studied isolates, altough the seven genotypes identified formed three main phylogenetic lineages. While four genotypes were identified amongst Norwegian lumpfish isolates, a single genotype dominated, irrespective of geographic origin. This suggests the existence of a dominant genotype associated with disease in production of lumpfish in Norwegian aquaculture. Elucidation of the population structure of the bacterium has provided valuable information for potential future vaccine development.
format	article
author	Even Bysveen Mjølnerød Hanne Katrine Nilsen Snore Gulla Andreas Riborg Kirsten Liland Bottolfsen Tom Wiklund Debes Christiansen Jesús Ángel López Romalde Felix Scholz Duncan John Colquhoun
author_facet	Even Bysveen Mjølnerød Hanne Katrine Nilsen Snore Gulla Andreas Riborg Kirsten Liland Bottolfsen Tom Wiklund Debes Christiansen Jesús Ángel López Romalde Felix Scholz Duncan John Colquhoun
author_sort	Even Bysveen Mjølnerød
title	Multilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).
title_short	Multilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).
title_full	Multilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).
title_fullStr	Multilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).
title_full_unstemmed	Multilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).
title_sort	multilocus sequence analysis reveals different lineages of pseudomonas anguilliseptica associated with disease in farmed lumpfish (cyclopterus lumpus l.).
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/e46c0c637e034186a9753e1f5629b3f6
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Multilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.).

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