Dysregulated autophagy contributes to podocyte damage in Fabry's disease.

Fabry's disease results from an inborn error of glycosphingolipid metabolism that is due to deficiency of the lysosomal hydrolase α-galactosidase A. This X-linked defect results in the accumulation of enzyme substrates with terminally α-glycosidically bound galactose, mainly the neutral glycosp...

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Autores principales:	Max C Liebau, Fabian Braun, Katja Höpker, Claudia Weitbrecht, Valerie Bartels, Roman-Ulrich Müller, Susanne Brodesser, Moin A Saleem, Thomas Benzing, Bernhard Schermer, Markus Cybulla, Christine E Kurschat
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e476685343814a1385f90b64d0282de0
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spelling	oai:doaj.org-article:e476685343814a1385f90b64d0282de02021-11-18T07:45:20ZDysregulated autophagy contributes to podocyte damage in Fabry's disease.1932-620310.1371/journal.pone.0063506https://doaj.org/article/e476685343814a1385f90b64d0282de02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23691056/?tool=EBIhttps://doaj.org/toc/1932-6203Fabry's disease results from an inborn error of glycosphingolipid metabolism that is due to deficiency of the lysosomal hydrolase α-galactosidase A. This X-linked defect results in the accumulation of enzyme substrates with terminally α-glycosidically bound galactose, mainly the neutral glycosphingolipid Globotriaosylceramide (Gb3) in various tissues, including the kidneys. Although end-stage renal disease is one of the most common causes of death in hemizygous males with Fabry's disease, the pathophysiology leading to proteinuria, hematuria, hypertension, and kidney failure is not well understood. Histological studies suggest that the accumulation of Gb3 in podocytes plays an important role in the pathogenesis of glomerular damage. However, due to the lack of appropriate animal or cellular models, podocyte damage in Fabry's disease could not be directly studied yet. As murine models are insufficient, a human model is needed. Here, we developed a human podocyte model of Fabry's disease by combining RNA interference technology with lentiviral transduction of human podocytes. Knockdown of α-galactosidase A expression resulted in diminished enzymatic activity and slowly progressive accumulation of intracellular Gb3. Interestingly, these changes were accompanied by an increase in autophagosomes as indicated by an increased abundance of LC3-II and a loss of mTOR kinase activity, a negative regulator of the autophagic machinery. These data suggest that dysregulated autophagy in α-galactosidase A-deficient podocytes may be the result of deficient mTOR kinase activity. This finding links the lysosomal enzymatic defect in Fabry's disease to deregulated autophagy pathways and provides a promising new direction for further studies on the pathomechanism of glomerular injury in Fabry patients.Max C LiebauFabian BraunKatja HöpkerClaudia WeitbrechtValerie BartelsRoman-Ulrich MüllerSusanne BrodesserMoin A SaleemThomas BenzingBernhard SchermerMarkus CybullaChristine E KurschatPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63506 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Max C Liebau Fabian Braun Katja Höpker Claudia Weitbrecht Valerie Bartels Roman-Ulrich Müller Susanne Brodesser Moin A Saleem Thomas Benzing Bernhard Schermer Markus Cybulla Christine E Kurschat Dysregulated autophagy contributes to podocyte damage in Fabry's disease.
description	Fabry's disease results from an inborn error of glycosphingolipid metabolism that is due to deficiency of the lysosomal hydrolase α-galactosidase A. This X-linked defect results in the accumulation of enzyme substrates with terminally α-glycosidically bound galactose, mainly the neutral glycosphingolipid Globotriaosylceramide (Gb3) in various tissues, including the kidneys. Although end-stage renal disease is one of the most common causes of death in hemizygous males with Fabry's disease, the pathophysiology leading to proteinuria, hematuria, hypertension, and kidney failure is not well understood. Histological studies suggest that the accumulation of Gb3 in podocytes plays an important role in the pathogenesis of glomerular damage. However, due to the lack of appropriate animal or cellular models, podocyte damage in Fabry's disease could not be directly studied yet. As murine models are insufficient, a human model is needed. Here, we developed a human podocyte model of Fabry's disease by combining RNA interference technology with lentiviral transduction of human podocytes. Knockdown of α-galactosidase A expression resulted in diminished enzymatic activity and slowly progressive accumulation of intracellular Gb3. Interestingly, these changes were accompanied by an increase in autophagosomes as indicated by an increased abundance of LC3-II and a loss of mTOR kinase activity, a negative regulator of the autophagic machinery. These data suggest that dysregulated autophagy in α-galactosidase A-deficient podocytes may be the result of deficient mTOR kinase activity. This finding links the lysosomal enzymatic defect in Fabry's disease to deregulated autophagy pathways and provides a promising new direction for further studies on the pathomechanism of glomerular injury in Fabry patients.
format	article
author	Max C Liebau Fabian Braun Katja Höpker Claudia Weitbrecht Valerie Bartels Roman-Ulrich Müller Susanne Brodesser Moin A Saleem Thomas Benzing Bernhard Schermer Markus Cybulla Christine E Kurschat
author_facet	Max C Liebau Fabian Braun Katja Höpker Claudia Weitbrecht Valerie Bartels Roman-Ulrich Müller Susanne Brodesser Moin A Saleem Thomas Benzing Bernhard Schermer Markus Cybulla Christine E Kurschat
author_sort	Max C Liebau
title	Dysregulated autophagy contributes to podocyte damage in Fabry's disease.
title_short	Dysregulated autophagy contributes to podocyte damage in Fabry's disease.
title_full	Dysregulated autophagy contributes to podocyte damage in Fabry's disease.
title_fullStr	Dysregulated autophagy contributes to podocyte damage in Fabry's disease.
title_full_unstemmed	Dysregulated autophagy contributes to podocyte damage in Fabry's disease.
title_sort	dysregulated autophagy contributes to podocyte damage in fabry's disease.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e476685343814a1385f90b64d0282de0
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Dysregulated autophagy contributes to podocyte damage in Fabry's disease.

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