Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels

Abstract Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers...

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Autores principales:	Soomin Chae, Hyun-Kyung Lee, Yoo-Kyung Kim, Hyo Jung Sim, Yoorim Ji, Chowon Kim, Tayaba Ismail, Jeen-Woo Park, Oh-Shin Kwon, Beom-Sik Kang, Dong-Seok Lee, Jong-Sup Bae, Sang-Hyun Kim, Kyoung-Jin Min, Taeg Kyu Kwon, Mae-Ja Park, Jin-Kwan Han, Taejoon Kwon, Tae-Joo Park, Hyun-Shik Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e477afed45f94379b672ac594535e46d
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spelling	oai:doaj.org-article:e477afed45f94379b672ac594535e46d2021-12-02T11:52:22ZPeroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels10.1038/s41598-017-09262-62045-2322https://doaj.org/article/e477afed45f94379b672ac594535e46d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09262-6https://doaj.org/toc/2045-2322Abstract Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H2O2) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.Soomin ChaeHyun-Kyung LeeYoo-Kyung KimHyo Jung SimYoorim JiChowon KimTayaba IsmailJeen-Woo ParkOh-Shin KwonBeom-Sik KangDong-Seok LeeJong-Sup BaeSang-Hyun KimKyoung-Jin MinTaeg Kyu KwonMae-Ja ParkJin-Kwan HanTaejoon KwonTae-Joo ParkHyun-Shik LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Soomin Chae Hyun-Kyung Lee Yoo-Kyung Kim Hyo Jung Sim Yoorim Ji Chowon Kim Tayaba Ismail Jeen-Woo Park Oh-Shin Kwon Beom-Sik Kang Dong-Seok Lee Jong-Sup Bae Sang-Hyun Kim Kyoung-Jin Min Taeg Kyu Kwon Mae-Ja Park Jin-Kwan Han Taejoon Kwon Tae-Joo Park Hyun-Shik Lee Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels
description	Abstract Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H2O2) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.
format	article
author	Soomin Chae Hyun-Kyung Lee Yoo-Kyung Kim Hyo Jung Sim Yoorim Ji Chowon Kim Tayaba Ismail Jeen-Woo Park Oh-Shin Kwon Beom-Sik Kang Dong-Seok Lee Jong-Sup Bae Sang-Hyun Kim Kyoung-Jin Min Taeg Kyu Kwon Mae-Ja Park Jin-Kwan Han Taejoon Kwon Tae-Joo Park Hyun-Shik Lee
author_facet	Soomin Chae Hyun-Kyung Lee Yoo-Kyung Kim Hyo Jung Sim Yoorim Ji Chowon Kim Tayaba Ismail Jeen-Woo Park Oh-Shin Kwon Beom-Sik Kang Dong-Seok Lee Jong-Sup Bae Sang-Hyun Kim Kyoung-Jin Min Taeg Kyu Kwon Mae-Ja Park Jin-Kwan Han Taejoon Kwon Tae-Joo Park Hyun-Shik Lee
author_sort	Soomin Chae
title	Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels
title_short	Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels
title_full	Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels
title_fullStr	Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels
title_full_unstemmed	Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels
title_sort	peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and wnt signaling by controlling ros levels
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e477afed45f94379b672ac594535e46d
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Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels

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