Insulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions

<i>Escherichia coli</i> plays an important role in biofilm formation across a wide array of disease and ecological settings. Insulin can function as an adjuvant in the regulation of biofilm levels. The modulation of insulin-regulated biofilm formation by environmental conditions has not...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nina Patel, Jeremy C. Curtis, Balbina J. Plotkin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>E. coli</i> biofilm insulin glucose galactose lactose Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/e4783be21cb647c5b1900620f84bbacb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e4783be21cb647c5b1900620f84bbacb
record_format	dspace
spelling	oai:doaj.org-article:e4783be21cb647c5b1900620f84bbacb2021-11-25T16:23:18ZInsulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions10.3390/antibiotics101113492079-6382https://doaj.org/article/e4783be21cb647c5b1900620f84bbacb2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1349https://doaj.org/toc/2079-6382<i>Escherichia coli</i> plays an important role in biofilm formation across a wide array of disease and ecological settings. Insulin can function as an adjuvant in the regulation of biofilm levels. The modulation of insulin-regulated biofilm formation by environmental conditions has not been previously described. In the present study, the effects that various environmental growth conditions and nutrients have on insulin-modulated levels of biofilm production were measured. Micropipette tips were incubated with <i>E. coli</i> ATCC<sup>®</sup> 25922™ in a Mueller Hinton broth (MH), or a yeast nitrogen base with 1% peptone (YNBP), which was supplemented with glucose, lactose, galactose and/or insulin (Humulin<sup>®</sup>-R). The incubation conditions included a shaking or static culture, at 23 °C or 37 °C. After incubation, the biofilm production was calculated per CFU. At 23 °C, the presence of insulin increased biofilm formation. The amount of biofilm formation was highest in glucose > galactose >> lactose, while the biofilm levels decreased in shaking cultures, except for galactose (3-fold increase; 0.1% galactose and 20 μU insulin). At 37 °C, regardless of condition, there was more biofilm formation/CFU under static conditions in YNBP than in MH, except for the MH containing galactose. <i>E. coli</i> biofilm formation is influenced by aeration, temperature, and insulin concentration in combination with the available sugars.Nina PatelJeremy C. CurtisBalbina J. PlotkinMDPI AGarticle<i>E. coli</i>biofilminsulinglucosegalactoselactoseTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1349, p 1349 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>E. coli</i> biofilm insulin glucose galactose lactose Therapeutics. Pharmacology RM1-950
spellingShingle	<i>E. coli</i> biofilm insulin glucose galactose lactose Therapeutics. Pharmacology RM1-950 Nina Patel Jeremy C. Curtis Balbina J. Plotkin Insulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions
description	<i>Escherichia coli</i> plays an important role in biofilm formation across a wide array of disease and ecological settings. Insulin can function as an adjuvant in the regulation of biofilm levels. The modulation of insulin-regulated biofilm formation by environmental conditions has not been previously described. In the present study, the effects that various environmental growth conditions and nutrients have on insulin-modulated levels of biofilm production were measured. Micropipette tips were incubated with <i>E. coli</i> ATCC<sup>®</sup> 25922™ in a Mueller Hinton broth (MH), or a yeast nitrogen base with 1% peptone (YNBP), which was supplemented with glucose, lactose, galactose and/or insulin (Humulin<sup>®</sup>-R). The incubation conditions included a shaking or static culture, at 23 °C or 37 °C. After incubation, the biofilm production was calculated per CFU. At 23 °C, the presence of insulin increased biofilm formation. The amount of biofilm formation was highest in glucose > galactose >> lactose, while the biofilm levels decreased in shaking cultures, except for galactose (3-fold increase; 0.1% galactose and 20 μU insulin). At 37 °C, regardless of condition, there was more biofilm formation/CFU under static conditions in YNBP than in MH, except for the MH containing galactose. <i>E. coli</i> biofilm formation is influenced by aeration, temperature, and insulin concentration in combination with the available sugars.
format	article
author	Nina Patel Jeremy C. Curtis Balbina J. Plotkin
author_facet	Nina Patel Jeremy C. Curtis Balbina J. Plotkin
author_sort	Nina Patel
title	Insulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions
title_short	Insulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions
title_full	Insulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions
title_fullStr	Insulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions
title_full_unstemmed	Insulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions
title_sort	insulin regulation of <i>escherichia coli</i> abiotic biofilm formation: effect of nutrients and growth conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e4783be21cb647c5b1900620f84bbacb
work_keys_str_mv	AT ninapatel insulinregulationofiescherichiacoliiabioticbiofilmformationeffectofnutrientsandgrowthconditions AT jeremyccurtis insulinregulationofiescherichiacoliiabioticbiofilmformationeffectofnutrientsandgrowthconditions AT balbinajplotkin insulinregulationofiescherichiacoliiabioticbiofilmformationeffectofnutrientsandgrowthconditions
_version_	1718413232702488576

Insulin Regulation of <i>Escherichia coli</i> Abiotic Biofilm Formation: Effect of Nutrients and Growth Conditions

Ejemplares similares