UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

The success of protein evolution is dependent on the sequence context mutations are introduced into. Here the authors present UMIC-seq that allows consensus generation for closely related genes by using unique molecular identifiers linked to gene variants.

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Autores principales:	Paul Jannis Zurek, Philipp Knyphausen, Katharina Neufeld, Ahir Pushpanath, Florian Hollfelder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e4783caa57744ab09ff2474ed8ee0e3c
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spelling	oai:doaj.org-article:e4783caa57744ab09ff2474ed8ee0e3c2021-12-02T17:31:24ZUMI-linked consensus sequencing enables phylogenetic analysis of directed evolution10.1038/s41467-020-19687-92041-1723https://doaj.org/article/e4783caa57744ab09ff2474ed8ee0e3c2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19687-9https://doaj.org/toc/2041-1723The success of protein evolution is dependent on the sequence context mutations are introduced into. Here the authors present UMIC-seq that allows consensus generation for closely related genes by using unique molecular identifiers linked to gene variants.Paul Jannis ZurekPhilipp KnyphausenKatharina NeufeldAhir PushpanathFlorian HollfelderNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Paul Jannis Zurek Philipp Knyphausen Katharina Neufeld Ahir Pushpanath Florian Hollfelder UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
description	The success of protein evolution is dependent on the sequence context mutations are introduced into. Here the authors present UMIC-seq that allows consensus generation for closely related genes by using unique molecular identifiers linked to gene variants.
format	article
author	Paul Jannis Zurek Philipp Knyphausen Katharina Neufeld Ahir Pushpanath Florian Hollfelder
author_facet	Paul Jannis Zurek Philipp Knyphausen Katharina Neufeld Ahir Pushpanath Florian Hollfelder
author_sort	Paul Jannis Zurek
title	UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_short	UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_full	UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_fullStr	UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_full_unstemmed	UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_sort	umi-linked consensus sequencing enables phylogenetic analysis of directed evolution
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e4783caa57744ab09ff2474ed8ee0e3c
work_keys_str_mv	AT pauljanniszurek umilinkedconsensussequencingenablesphylogeneticanalysisofdirectedevolution AT philippknyphausen umilinkedconsensussequencingenablesphylogeneticanalysisofdirectedevolution AT katharinaneufeld umilinkedconsensussequencingenablesphylogeneticanalysisofdirectedevolution AT ahirpushpanath umilinkedconsensussequencingenablesphylogeneticanalysisofdirectedevolution AT florianhollfelder umilinkedconsensussequencingenablesphylogeneticanalysisofdirectedevolution
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UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

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