Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans
Abstract Human blood metagenomics has revealed the presence of different types of viruses in apparently healthy subjects. By far, anelloviruses constitute the viral family that is more frequently found in human blood, although amplification biases and contaminations pose a major challenge in this fi...
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Nature Portfolio
2021
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oai:doaj.org-article:e4799266aa524b499aa4889eff091ca92021-12-02T16:36:04ZDeep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans10.1038/s41598-021-86427-42045-2322https://doaj.org/article/e4799266aa524b499aa4889eff091ca92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86427-4https://doaj.org/toc/2045-2322Abstract Human blood metagenomics has revealed the presence of different types of viruses in apparently healthy subjects. By far, anelloviruses constitute the viral family that is more frequently found in human blood, although amplification biases and contaminations pose a major challenge in this field. To investigate this further, we subjected pooled plasma samples from 120 healthy donors in Spain to high-speed centrifugation, RNA and DNA extraction, random amplification, and massive parallel sequencing. Our results confirm the extensive presence of anelloviruses in such samples, which represented nearly 97% of the total viral sequence reads obtained. We assembled 114 different viral genomes belonging to this family, revealing remarkable diversity. Phylogenetic analysis of ORF1 suggested 28 potentially novel anellovirus species, 24 of which were validated by Sanger sequencing to discard artifacts. These findings underscore the importance of implementing more efficient purification procedures that enrich the viral fraction as an essential step in virome studies and question the suggested pathological role of anelloviruses.María Cebriá-MendozaCristina ArbonaLuís LarreaWladimiro DíazVicente ArnauCarlos PeñaJuan Vicente BouRafael SanjuánJosé M. CuevasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q María Cebriá-Mendoza Cristina Arbona Luís Larrea Wladimiro Díaz Vicente Arnau Carlos Peña Juan Vicente Bou Rafael Sanjuán José M. Cuevas Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans |
| description |
Abstract Human blood metagenomics has revealed the presence of different types of viruses in apparently healthy subjects. By far, anelloviruses constitute the viral family that is more frequently found in human blood, although amplification biases and contaminations pose a major challenge in this field. To investigate this further, we subjected pooled plasma samples from 120 healthy donors in Spain to high-speed centrifugation, RNA and DNA extraction, random amplification, and massive parallel sequencing. Our results confirm the extensive presence of anelloviruses in such samples, which represented nearly 97% of the total viral sequence reads obtained. We assembled 114 different viral genomes belonging to this family, revealing remarkable diversity. Phylogenetic analysis of ORF1 suggested 28 potentially novel anellovirus species, 24 of which were validated by Sanger sequencing to discard artifacts. These findings underscore the importance of implementing more efficient purification procedures that enrich the viral fraction as an essential step in virome studies and question the suggested pathological role of anelloviruses. |
| format |
article |
| author |
María Cebriá-Mendoza Cristina Arbona Luís Larrea Wladimiro Díaz Vicente Arnau Carlos Peña Juan Vicente Bou Rafael Sanjuán José M. Cuevas |
| author_facet |
María Cebriá-Mendoza Cristina Arbona Luís Larrea Wladimiro Díaz Vicente Arnau Carlos Peña Juan Vicente Bou Rafael Sanjuán José M. Cuevas |
| author_sort |
María Cebriá-Mendoza |
| title |
Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans |
| title_short |
Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans |
| title_full |
Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans |
| title_fullStr |
Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans |
| title_full_unstemmed |
Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans |
| title_sort |
deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/e4799266aa524b499aa4889eff091ca9 |
| work_keys_str_mv |
AT mariacebriamendoza deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans AT cristinaarbona deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans AT luislarrea deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans AT wladimirodiaz deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans AT vicentearnau deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans AT carlospena deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans AT juanvicentebou deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans AT rafaelsanjuan deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans AT josemcuevas deepviralbloodmetagenomicsrevealsextensiveanellovirusdiversityinhealthyhumans |
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