Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients.
Several studies in mouse model of invasive aspergillosis (IA) and in healthy donors have shown that different Aspergillus antigens may stimulate different adaptive immune responses. However, the occurrence of Aspergillus-specific T cells have not yet been reported in patients with the disease. In pa...
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oai:doaj.org-article:e47bb1abb7c949d98af917432aeb935f2021-11-18T08:56:52ZCharacterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients.1932-620310.1371/journal.pone.0074326https://doaj.org/article/e47bb1abb7c949d98af917432aeb935f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24023936/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Several studies in mouse model of invasive aspergillosis (IA) and in healthy donors have shown that different Aspergillus antigens may stimulate different adaptive immune responses. However, the occurrence of Aspergillus-specific T cells have not yet been reported in patients with the disease. In patients with IA, we have investigated during the infection: a) whether and how specific T-cell responses to different Aspergillus antigens occur and develop; b) which antigens elicit the highest frequencies of protective immune responses and, c) whether such protective T cells could be expanded ex-vivo. Forty hematologic patients have been studied, including 22 patients with IA and 18 controls. Specific T cells producing IL-10, IFN-γ, IL-4 and IL-17A have been characterized through enzyme linked immunospot and cytokine secretion assays on 88 peripheral blood (PB) samples, by using the following recombinant antigens: GEL1p, CRF1p, PEP1p, SOD1p, α1-3glucan, β1-3glucan, galactomannan. Specific T cells were expanded through short term culture. Aspergillus-specific T cells producing non-protective interleukin-10 (IL-10) and protective interferon-gamma (IFN-γ) have been detected to all the antigens only in IA patients. Lower numbers of specific T cells producing IL-4 and IL-17A have also been shown. Protective T cells targeted predominantly Aspergillus cell wall antigens, tended to increase during the IA course and to be associated with a better clinical outcome. Aspergillus-specific T cells could be successfully generated from the PB of 8 out of 8 patients with IA and included cytotoxic subsets able to lyse Aspergillus hyphae. Aspergillus specific T-cell responses contribute to the clearance of the pathogen in immunosuppressed patients with IA and Aspergillus cell wall antigens are those mainly targeted by protective immune responses. Cytotoxic specific T cells can be expanded from immunosuppressed patients even during the infection by using the above mentioned antigens. These findings may be exploited for immunotherapeutic purposes in patients with IA.Leonardo PotenzaDaniela ValleriniPatrizia BarozziGiovanni RivaFabio ForghieriAnne BeauvaisRemi BeauAnna CandoniJohan MaertensGiulio RossiGiulio RossiMonica MorselliEleonora ZanettiChiara QuadrelliMauro CodeluppiGiovanni GuaraldiLivio PaganoMorena CairaCinzia Del GiovaneMonica MaccaferriAlessandro StefaniUliano MorandiGiovanni TazzioliMassimo GirardisMario DeliaGiorgina SpecchiaGiuseppe LongoRoberto MarascaFranco NarniFrancesco MerliAnnalisa ImovilliGiovanni ApoloneAgostinho CarvalhoPatrizia ComoliLuigina RomaniJean Paul LatgèMario LuppiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74326 (2013) |
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Medicine R Science Q Leonardo Potenza Daniela Vallerini Patrizia Barozzi Giovanni Riva Fabio Forghieri Anne Beauvais Remi Beau Anna Candoni Johan Maertens Giulio Rossi Giulio Rossi Monica Morselli Eleonora Zanetti Chiara Quadrelli Mauro Codeluppi Giovanni Guaraldi Livio Pagano Morena Caira Cinzia Del Giovane Monica Maccaferri Alessandro Stefani Uliano Morandi Giovanni Tazzioli Massimo Girardis Mario Delia Giorgina Specchia Giuseppe Longo Roberto Marasca Franco Narni Francesco Merli Annalisa Imovilli Giovanni Apolone Agostinho Carvalho Patrizia Comoli Luigina Romani Jean Paul Latgè Mario Luppi Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients. |
| description |
Several studies in mouse model of invasive aspergillosis (IA) and in healthy donors have shown that different Aspergillus antigens may stimulate different adaptive immune responses. However, the occurrence of Aspergillus-specific T cells have not yet been reported in patients with the disease. In patients with IA, we have investigated during the infection: a) whether and how specific T-cell responses to different Aspergillus antigens occur and develop; b) which antigens elicit the highest frequencies of protective immune responses and, c) whether such protective T cells could be expanded ex-vivo. Forty hematologic patients have been studied, including 22 patients with IA and 18 controls. Specific T cells producing IL-10, IFN-γ, IL-4 and IL-17A have been characterized through enzyme linked immunospot and cytokine secretion assays on 88 peripheral blood (PB) samples, by using the following recombinant antigens: GEL1p, CRF1p, PEP1p, SOD1p, α1-3glucan, β1-3glucan, galactomannan. Specific T cells were expanded through short term culture. Aspergillus-specific T cells producing non-protective interleukin-10 (IL-10) and protective interferon-gamma (IFN-γ) have been detected to all the antigens only in IA patients. Lower numbers of specific T cells producing IL-4 and IL-17A have also been shown. Protective T cells targeted predominantly Aspergillus cell wall antigens, tended to increase during the IA course and to be associated with a better clinical outcome. Aspergillus-specific T cells could be successfully generated from the PB of 8 out of 8 patients with IA and included cytotoxic subsets able to lyse Aspergillus hyphae. Aspergillus specific T-cell responses contribute to the clearance of the pathogen in immunosuppressed patients with IA and Aspergillus cell wall antigens are those mainly targeted by protective immune responses. Cytotoxic specific T cells can be expanded from immunosuppressed patients even during the infection by using the above mentioned antigens. These findings may be exploited for immunotherapeutic purposes in patients with IA. |
| format |
article |
| author |
Leonardo Potenza Daniela Vallerini Patrizia Barozzi Giovanni Riva Fabio Forghieri Anne Beauvais Remi Beau Anna Candoni Johan Maertens Giulio Rossi Giulio Rossi Monica Morselli Eleonora Zanetti Chiara Quadrelli Mauro Codeluppi Giovanni Guaraldi Livio Pagano Morena Caira Cinzia Del Giovane Monica Maccaferri Alessandro Stefani Uliano Morandi Giovanni Tazzioli Massimo Girardis Mario Delia Giorgina Specchia Giuseppe Longo Roberto Marasca Franco Narni Francesco Merli Annalisa Imovilli Giovanni Apolone Agostinho Carvalho Patrizia Comoli Luigina Romani Jean Paul Latgè Mario Luppi |
| author_facet |
Leonardo Potenza Daniela Vallerini Patrizia Barozzi Giovanni Riva Fabio Forghieri Anne Beauvais Remi Beau Anna Candoni Johan Maertens Giulio Rossi Giulio Rossi Monica Morselli Eleonora Zanetti Chiara Quadrelli Mauro Codeluppi Giovanni Guaraldi Livio Pagano Morena Caira Cinzia Del Giovane Monica Maccaferri Alessandro Stefani Uliano Morandi Giovanni Tazzioli Massimo Girardis Mario Delia Giorgina Specchia Giuseppe Longo Roberto Marasca Franco Narni Francesco Merli Annalisa Imovilli Giovanni Apolone Agostinho Carvalho Patrizia Comoli Luigina Romani Jean Paul Latgè Mario Luppi |
| author_sort |
Leonardo Potenza |
| title |
Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients. |
| title_short |
Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients. |
| title_full |
Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients. |
| title_fullStr |
Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients. |
| title_full_unstemmed |
Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients. |
| title_sort |
characterization of specific immune responses to different aspergillus antigens during the course of invasive aspergillosis in hematologic patients. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/e47bb1abb7c949d98af917432aeb935f |
| work_keys_str_mv |
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