Impact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia
Strain-related differences in arteriogenesis in inbred mouse strains have already been studied excessively. However, these analyses missed evaluating the mouse strain-related differences in ischemia-induced angiogenic capacities. With the present study, we wanted to shed light on the different angio...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/e47cd0bc8575413b8d563a2daedb3cfb |
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| Sumario: | Strain-related differences in arteriogenesis in inbred mouse strains have already been studied excessively. However, these analyses missed evaluating the mouse strain-related differences in ischemia-induced angiogenic capacities. With the present study, we wanted to shed light on the different angiogenic potentials and the associated leukocyte infiltration of <i>C57BL/6J</i> and <i>SV-129</i> mice to facilitate the comparison of angiogenesis-related analyses between these strains. For the induction of angiogenesis, we ligated the femoral artery in 8–12-week-old male <i>C57BL/6J</i> and <i>SV-129</i> mice and performed (immuno-) histological analyses on the ischemic gastrocnemius muscles collected 24 h or 7 days after ligation. As evidenced by hematoxylin and eosin staining, <i>C57BL/6J</i> mice showed reduced tissue damage but displayed an increased capillary-to-muscle fiber ratio and an elevated number of proliferating capillaries (CD31<sup>+</sup>/BrdU<sup>+</sup> cells) compared to <i>SV-129</i> mice, thus showing improved angiogenesis. Regarding the associated leukocyte infiltration, we found increased numbers of neutrophils (MPO<sup>+</sup> cells), NETs (MPO<sup>+</sup>/CitH3<sup>+</sup>/DAPI<sup>+</sup>), and macrophages (CD68<sup>+</sup> cells) in <i>SV-129</i> mice, whereas macrophage polarization (MRC1<sup>-</sup> vs. MRC1<sup>+</sup>) and total leukocyte infiltration (CD45<sup>+</sup> cells) did not differ between the mouse strains. In summary, we show increased ischemia-induced angiogenic capacities in <i>C57BL/6J</i> mice compared to <i>SV-129</i> mice, with the latter showing aggravated tissue damage, inflammation, and impaired angiogenesis. |
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