Impact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia
Strain-related differences in arteriogenesis in inbred mouse strains have already been studied excessively. However, these analyses missed evaluating the mouse strain-related differences in ischemia-induced angiogenic capacities. With the present study, we wanted to shed light on the different angio...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e47cd0bc8575413b8d563a2daedb3cfb |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e47cd0bc8575413b8d563a2daedb3cfb |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e47cd0bc8575413b8d563a2daedb3cfb2021-11-11T17:14:43ZImpact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia10.3390/ijms2221117951422-00671661-6596https://doaj.org/article/e47cd0bc8575413b8d563a2daedb3cfb2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11795https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Strain-related differences in arteriogenesis in inbred mouse strains have already been studied excessively. However, these analyses missed evaluating the mouse strain-related differences in ischemia-induced angiogenic capacities. With the present study, we wanted to shed light on the different angiogenic potentials and the associated leukocyte infiltration of <i>C57BL/6J</i> and <i>SV-129</i> mice to facilitate the comparison of angiogenesis-related analyses between these strains. For the induction of angiogenesis, we ligated the femoral artery in 8–12-week-old male <i>C57BL/6J</i> and <i>SV-129</i> mice and performed (immuno-) histological analyses on the ischemic gastrocnemius muscles collected 24 h or 7 days after ligation. As evidenced by hematoxylin and eosin staining, <i>C57BL/6J</i> mice showed reduced tissue damage but displayed an increased capillary-to-muscle fiber ratio and an elevated number of proliferating capillaries (CD31<sup>+</sup>/BrdU<sup>+</sup> cells) compared to <i>SV-129</i> mice, thus showing improved angiogenesis. Regarding the associated leukocyte infiltration, we found increased numbers of neutrophils (MPO<sup>+</sup> cells), NETs (MPO<sup>+</sup>/CitH3<sup>+</sup>/DAPI<sup>+</sup>), and macrophages (CD68<sup>+</sup> cells) in <i>SV-129</i> mice, whereas macrophage polarization (MRC1<sup>-</sup> vs. MRC1<sup>+</sup>) and total leukocyte infiltration (CD45<sup>+</sup> cells) did not differ between the mouse strains. In summary, we show increased ischemia-induced angiogenic capacities in <i>C57BL/6J</i> mice compared to <i>SV-129</i> mice, with the latter showing aggravated tissue damage, inflammation, and impaired angiogenesis.Matthias KüblerPhilipp GötzAnna BraumandlSebastian BeckHellen Ishikawa-AnkerholdElisabeth DeindlMDPI AGarticleangiogenesis<i>C57BL/6J</i> mice<i>SV-129</i> miceleukocytesmacrophagesneutrophilsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11795, p 11795 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
angiogenesis <i>C57BL/6J</i> mice <i>SV-129</i> mice leukocytes macrophages neutrophils Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
angiogenesis <i>C57BL/6J</i> mice <i>SV-129</i> mice leukocytes macrophages neutrophils Biology (General) QH301-705.5 Chemistry QD1-999 Matthias Kübler Philipp Götz Anna Braumandl Sebastian Beck Hellen Ishikawa-Ankerhold Elisabeth Deindl Impact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia |
| description |
Strain-related differences in arteriogenesis in inbred mouse strains have already been studied excessively. However, these analyses missed evaluating the mouse strain-related differences in ischemia-induced angiogenic capacities. With the present study, we wanted to shed light on the different angiogenic potentials and the associated leukocyte infiltration of <i>C57BL/6J</i> and <i>SV-129</i> mice to facilitate the comparison of angiogenesis-related analyses between these strains. For the induction of angiogenesis, we ligated the femoral artery in 8–12-week-old male <i>C57BL/6J</i> and <i>SV-129</i> mice and performed (immuno-) histological analyses on the ischemic gastrocnemius muscles collected 24 h or 7 days after ligation. As evidenced by hematoxylin and eosin staining, <i>C57BL/6J</i> mice showed reduced tissue damage but displayed an increased capillary-to-muscle fiber ratio and an elevated number of proliferating capillaries (CD31<sup>+</sup>/BrdU<sup>+</sup> cells) compared to <i>SV-129</i> mice, thus showing improved angiogenesis. Regarding the associated leukocyte infiltration, we found increased numbers of neutrophils (MPO<sup>+</sup> cells), NETs (MPO<sup>+</sup>/CitH3<sup>+</sup>/DAPI<sup>+</sup>), and macrophages (CD68<sup>+</sup> cells) in <i>SV-129</i> mice, whereas macrophage polarization (MRC1<sup>-</sup> vs. MRC1<sup>+</sup>) and total leukocyte infiltration (CD45<sup>+</sup> cells) did not differ between the mouse strains. In summary, we show increased ischemia-induced angiogenic capacities in <i>C57BL/6J</i> mice compared to <i>SV-129</i> mice, with the latter showing aggravated tissue damage, inflammation, and impaired angiogenesis. |
| format |
article |
| author |
Matthias Kübler Philipp Götz Anna Braumandl Sebastian Beck Hellen Ishikawa-Ankerhold Elisabeth Deindl |
| author_facet |
Matthias Kübler Philipp Götz Anna Braumandl Sebastian Beck Hellen Ishikawa-Ankerhold Elisabeth Deindl |
| author_sort |
Matthias Kübler |
| title |
Impact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia |
| title_short |
Impact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia |
| title_full |
Impact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia |
| title_fullStr |
Impact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia |
| title_full_unstemmed |
Impact of <i>C57BL/6J</i> and <i>SV-129</i> Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia |
| title_sort |
impact of <i>c57bl/6j</i> and <i>sv-129</i> mouse strain differences on ischemia-induced postnatal angiogenesis and the associated leukocyte infiltration in a murine hindlimb model of ischemia |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/e47cd0bc8575413b8d563a2daedb3cfb |
| work_keys_str_mv |
AT matthiaskubler impactofic57bl6jiandisv129imousestraindifferencesonischemiainducedpostnatalangiogenesisandtheassociatedleukocyteinfiltrationinamurinehindlimbmodelofischemia AT philippgotz impactofic57bl6jiandisv129imousestraindifferencesonischemiainducedpostnatalangiogenesisandtheassociatedleukocyteinfiltrationinamurinehindlimbmodelofischemia AT annabraumandl impactofic57bl6jiandisv129imousestraindifferencesonischemiainducedpostnatalangiogenesisandtheassociatedleukocyteinfiltrationinamurinehindlimbmodelofischemia AT sebastianbeck impactofic57bl6jiandisv129imousestraindifferencesonischemiainducedpostnatalangiogenesisandtheassociatedleukocyteinfiltrationinamurinehindlimbmodelofischemia AT hellenishikawaankerhold impactofic57bl6jiandisv129imousestraindifferencesonischemiainducedpostnatalangiogenesisandtheassociatedleukocyteinfiltrationinamurinehindlimbmodelofischemia AT elisabethdeindl impactofic57bl6jiandisv129imousestraindifferencesonischemiainducedpostnatalangiogenesisandtheassociatedleukocyteinfiltrationinamurinehindlimbmodelofischemia |
| _version_ |
1718432150994288640 |