Evolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.

Recent studies suggest that members of the Microviridae (a family of ssDNA bacteriophages) might play an important role in a broad spectrum of environments, as they were found in great number among the viral fraction from seawater and human gut samples. 24 completely sequenced Microviridae have been...

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Autores principales:	Simon Roux, Mart Krupovic, Axel Poulet, Didier Debroas, François Enault
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e48d7f57fe1d47d89c12e5fd76fa2b9a
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spelling	oai:doaj.org-article:e48d7f57fe1d47d89c12e5fd76fa2b9a2021-11-18T07:12:45ZEvolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.1932-620310.1371/journal.pone.0040418https://doaj.org/article/e48d7f57fe1d47d89c12e5fd76fa2b9a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22808158/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recent studies suggest that members of the Microviridae (a family of ssDNA bacteriophages) might play an important role in a broad spectrum of environments, as they were found in great number among the viral fraction from seawater and human gut samples. 24 completely sequenced Microviridae have been described so far, divided into three distinct groups named Microvirus, Gokushovirinae and Alpavirinae, this last group being only composed of prophages. In this study, we present the analysis of 81 new complete Microviridae genomes, assembled from viral metagenomes originating from various ecosystems. The phylogenetic analysis of the core genes highlights the existence of four groups, confirming the three sub-families described so far and exhibiting a new group, named Pichovirinae. The genomic organizations of these viruses are strikingly coherent with their phylogeny, the Pichovirinae being the only group of this family with a different organization of the three core genes. Analysis of the structure of the major capsid protein reveals the presence of mushroom-like insertions conserved within all the groups except for the microviruses. In addition, a peptidase gene was found in 10 Microviridae and its analysis indicates a horizontal gene transfer that occurred several times between these viruses and their bacterial hosts. This is the first report of such gene transfer in Microviridae. Finally, searches against viral metagenomes revealed the presence of highly similar sequences in a variety of biomes indicating that Microviridae probably have both an important role in these ecosystems and an ancient origin.Simon RouxMart KrupovicAxel PouletDidier DebroasFrançois EnaultPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40418 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simon Roux Mart Krupovic Axel Poulet Didier Debroas François Enault Evolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.
description	Recent studies suggest that members of the Microviridae (a family of ssDNA bacteriophages) might play an important role in a broad spectrum of environments, as they were found in great number among the viral fraction from seawater and human gut samples. 24 completely sequenced Microviridae have been described so far, divided into three distinct groups named Microvirus, Gokushovirinae and Alpavirinae, this last group being only composed of prophages. In this study, we present the analysis of 81 new complete Microviridae genomes, assembled from viral metagenomes originating from various ecosystems. The phylogenetic analysis of the core genes highlights the existence of four groups, confirming the three sub-families described so far and exhibiting a new group, named Pichovirinae. The genomic organizations of these viruses are strikingly coherent with their phylogeny, the Pichovirinae being the only group of this family with a different organization of the three core genes. Analysis of the structure of the major capsid protein reveals the presence of mushroom-like insertions conserved within all the groups except for the microviruses. In addition, a peptidase gene was found in 10 Microviridae and its analysis indicates a horizontal gene transfer that occurred several times between these viruses and their bacterial hosts. This is the first report of such gene transfer in Microviridae. Finally, searches against viral metagenomes revealed the presence of highly similar sequences in a variety of biomes indicating that Microviridae probably have both an important role in these ecosystems and an ancient origin.
format	article
author	Simon Roux Mart Krupovic Axel Poulet Didier Debroas François Enault
author_facet	Simon Roux Mart Krupovic Axel Poulet Didier Debroas François Enault
author_sort	Simon Roux
title	Evolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.
title_short	Evolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.
title_full	Evolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.
title_fullStr	Evolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.
title_full_unstemmed	Evolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.
title_sort	evolution and diversity of the microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e48d7f57fe1d47d89c12e5fd76fa2b9a
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Evolution and diversity of the Microviridae viral family through a collection of 81 new complete genomes assembled from virome reads.

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