Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes

Brassica yellows virus (BrYV) is a tentative species of the genus <i>Polerovirus</i>, which has at least three genotypes (A, B, and C) in China. The P0 protein of BrYV-A (P0<sup>BrA</sup>) has been identified as a viral suppressor of RNA silencing (VSR), which can also induce...

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Autores principales:	Xiao-Yan Zhang, Yuan-Yuan Li, Ying Wang, Da-Wei Li, Jia-Lin Yu, Cheng-Gui Han
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Brassica yellows virus P0 RNA silencing suppressor cell death Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e48f250190b24bb7904bcd71803027ed
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spelling	oai:doaj.org-article:e48f250190b24bb7904bcd71803027ed2021-11-25T16:46:47ZComparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes10.3390/biology101110762079-7737https://doaj.org/article/e48f250190b24bb7904bcd71803027ed2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1076https://doaj.org/toc/2079-7737Brassica yellows virus (BrYV) is a tentative species of the genus <i>Polerovirus</i>, which has at least three genotypes (A, B, and C) in China. The P0 protein of BrYV-A (P0<sup>BrA</sup>) has been identified as a viral suppressor of RNA silencing (VSR), which can also induce cell death in infiltrated <i>Nicotiana benthamiana</i> leaves. In this study, we demonstrated that the cell death induced by P0<sup>BrA</sup> was accompanied by the accumulation of reactive oxygen species (ROS) and increased <i>Pathogenesis-related protein genes-1</i> (<i>PR1</i>) expression. Meanwhile, this cell death phenotype was delayed by salicylic acid (SA) pretreatment. Biological function comparison of the three P0 proteins showed that transiently expressed P0<sup>BrB</sup> or P0<sup>BrC</sup> induced a significantly delayed and milder cell death response compared with P0<sup>BrA</sup>. However, like P0<sup>BrA</sup>, they also suppressed local and systemic RNA silencing. Six residues of P0<sup>BrA</sup> essential for inducing cell death were identified by comparative analysis and amino acid substitution assay. We also show that all three BrYV genotypes have synergistic interactions with pea enation mosaic virus 2 (PEMV 2) in <i>N. benthamiana</i>. This study provides theoretical guidance for controlling the viral disease caused by poleroviruses in the future.Xiao-Yan ZhangYuan-Yuan LiYing WangDa-Wei LiJia-Lin YuCheng-Gui HanMDPI AGarticleBrassica yellows virusP0RNA silencing suppressorcell deathBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1076, p 1076 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Brassica yellows virus P0 RNA silencing suppressor cell death Biology (General) QH301-705.5
spellingShingle	Brassica yellows virus P0 RNA silencing suppressor cell death Biology (General) QH301-705.5 Xiao-Yan Zhang Yuan-Yuan Li Ying Wang Da-Wei Li Jia-Lin Yu Cheng-Gui Han Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes
description	Brassica yellows virus (BrYV) is a tentative species of the genus <i>Polerovirus</i>, which has at least three genotypes (A, B, and C) in China. The P0 protein of BrYV-A (P0<sup>BrA</sup>) has been identified as a viral suppressor of RNA silencing (VSR), which can also induce cell death in infiltrated <i>Nicotiana benthamiana</i> leaves. In this study, we demonstrated that the cell death induced by P0<sup>BrA</sup> was accompanied by the accumulation of reactive oxygen species (ROS) and increased <i>Pathogenesis-related protein genes-1</i> (<i>PR1</i>) expression. Meanwhile, this cell death phenotype was delayed by salicylic acid (SA) pretreatment. Biological function comparison of the three P0 proteins showed that transiently expressed P0<sup>BrB</sup> or P0<sup>BrC</sup> induced a significantly delayed and milder cell death response compared with P0<sup>BrA</sup>. However, like P0<sup>BrA</sup>, they also suppressed local and systemic RNA silencing. Six residues of P0<sup>BrA</sup> essential for inducing cell death were identified by comparative analysis and amino acid substitution assay. We also show that all three BrYV genotypes have synergistic interactions with pea enation mosaic virus 2 (PEMV 2) in <i>N. benthamiana</i>. This study provides theoretical guidance for controlling the viral disease caused by poleroviruses in the future.
format	article
author	Xiao-Yan Zhang Yuan-Yuan Li Ying Wang Da-Wei Li Jia-Lin Yu Cheng-Gui Han
author_facet	Xiao-Yan Zhang Yuan-Yuan Li Ying Wang Da-Wei Li Jia-Lin Yu Cheng-Gui Han
author_sort	Xiao-Yan Zhang
title	Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes
title_short	Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes
title_full	Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes
title_fullStr	Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes
title_full_unstemmed	Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes
title_sort	comparative analysis of biological characteristics among p0 proteins from different brassica yellows virus genotypes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e48f250190b24bb7904bcd71803027ed
work_keys_str_mv	AT xiaoyanzhang comparativeanalysisofbiologicalcharacteristicsamongp0proteinsfromdifferentbrassicayellowsvirusgenotypes AT yuanyuanli comparativeanalysisofbiologicalcharacteristicsamongp0proteinsfromdifferentbrassicayellowsvirusgenotypes AT yingwang comparativeanalysisofbiologicalcharacteristicsamongp0proteinsfromdifferentbrassicayellowsvirusgenotypes AT daweili comparativeanalysisofbiologicalcharacteristicsamongp0proteinsfromdifferentbrassicayellowsvirusgenotypes AT jialinyu comparativeanalysisofbiologicalcharacteristicsamongp0proteinsfromdifferentbrassicayellowsvirusgenotypes AT chengguihan comparativeanalysisofbiologicalcharacteristicsamongp0proteinsfromdifferentbrassicayellowsvirusgenotypes
_version_	1718412989755817984

Comparative Analysis of Biological Characteristics among P0 Proteins from Different Brassica Yellows Virus Genotypes

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