MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11

Seminal plasma contains a large number of extracellular vesicles (EVs). However, the roles of these EVs and their interactions with sperm are not clear. To identify the important molecules affecting sperm motility in EVs, we analyzed RNA from seminal plasma EVs of boars with different sperm motility...

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Autores principales:	Yaqun Ding, Ning Ding, Yu Zhang, Shenmin Xie, Mengna Huang, Xiangdong Ding, Wuzi Dong, Qin Zhang, Li Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	extracellular vesicles miR-222 sperm motility EGFR BCL2L11 apoptosis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e4910ebc3b894e22a0503c76ec38b8d3
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spelling	oai:doaj.org-article:e4910ebc3b894e22a0503c76ec38b8d32021-11-08T13:33:41ZMicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L112296-634X10.3389/fcell.2021.736864https://doaj.org/article/e4910ebc3b894e22a0503c76ec38b8d32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.736864/fullhttps://doaj.org/toc/2296-634XSeminal plasma contains a large number of extracellular vesicles (EVs). However, the roles of these EVs and their interactions with sperm are not clear. To identify the important molecules affecting sperm motility in EVs, we analyzed RNA from seminal plasma EVs of boars with different sperm motility using whole-transcriptome sequencing and proteomic analysis. In total, 7 miRNAs, 67 lncRNAs, 126 mRNAs and 76 proteins were differentially expressed between the two groups. We observed that EV-miR-222 can obviously improve sperm motility. In addition, the results suggested that miR-222 was transferred into sperm by the EVs and that miR-222 affected sperm apoptosis by inhibiting the expression of EGFR, BCL2L11, BAX, CYCs, CASP9 and CASP3. The results of electron microscopy also showed that overexpression of miR-222 in EVs could reduce sperm apoptosis. The study of the whole transcriptomes and proteomes of EVs in boar semen revealed some miRNAs may play an important role in these EVs interactions with Duroc sperm, and the findings suggest that the release of miR-222 by semen EVs is an important mechanism by which sperm viability is maintained and sperm apoptosis is reduced. Our studies provide a new insight of miR-222 in EVs regulation for sperm motility and sperm apoptosis.Yaqun DingNing DingYu ZhangShenmin XieMengna HuangXiangdong DingWuzi DongQin ZhangQin ZhangLi JiangFrontiers Media S.A.articleextracellular vesiclesmiR-222sperm motilityEGFRBCL2L11apoptosisBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular vesicles miR-222 sperm motility EGFR BCL2L11 apoptosis Biology (General) QH301-705.5
spellingShingle	extracellular vesicles miR-222 sperm motility EGFR BCL2L11 apoptosis Biology (General) QH301-705.5 Yaqun Ding Ning Ding Yu Zhang Shenmin Xie Mengna Huang Xiangdong Ding Wuzi Dong Qin Zhang Qin Zhang Li Jiang MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11
description	Seminal plasma contains a large number of extracellular vesicles (EVs). However, the roles of these EVs and their interactions with sperm are not clear. To identify the important molecules affecting sperm motility in EVs, we analyzed RNA from seminal plasma EVs of boars with different sperm motility using whole-transcriptome sequencing and proteomic analysis. In total, 7 miRNAs, 67 lncRNAs, 126 mRNAs and 76 proteins were differentially expressed between the two groups. We observed that EV-miR-222 can obviously improve sperm motility. In addition, the results suggested that miR-222 was transferred into sperm by the EVs and that miR-222 affected sperm apoptosis by inhibiting the expression of EGFR, BCL2L11, BAX, CYCs, CASP9 and CASP3. The results of electron microscopy also showed that overexpression of miR-222 in EVs could reduce sperm apoptosis. The study of the whole transcriptomes and proteomes of EVs in boar semen revealed some miRNAs may play an important role in these EVs interactions with Duroc sperm, and the findings suggest that the release of miR-222 by semen EVs is an important mechanism by which sperm viability is maintained and sperm apoptosis is reduced. Our studies provide a new insight of miR-222 in EVs regulation for sperm motility and sperm apoptosis.
format	article
author	Yaqun Ding Ning Ding Yu Zhang Shenmin Xie Mengna Huang Xiangdong Ding Wuzi Dong Qin Zhang Qin Zhang Li Jiang
author_facet	Yaqun Ding Ning Ding Yu Zhang Shenmin Xie Mengna Huang Xiangdong Ding Wuzi Dong Qin Zhang Qin Zhang Li Jiang
author_sort	Yaqun Ding
title	MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11
title_short	MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11
title_full	MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11
title_fullStr	MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11
title_full_unstemmed	MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11
title_sort	microrna-222 transferred from semen extracellular vesicles inhibits sperm apoptosis by targeting bcl2l11
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e4910ebc3b894e22a0503c76ec38b8d3
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MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11

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