Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors

Chemoattraction is a normal and essential process, but it can also be involved in tumorigenesis. This phenomenon plays a key role in glioblastoma (GBM). The GBM tumor cells are extremely difficult to eradicate, due to their strong capacity to migrate into the brain parenchyma. Consequently, a comple...

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Autores principales:	Laurence Déry, Gabriel Charest, Brigitte Guérin, Mohsen Akbari, David Fortin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	neuro-oncology brain tumor glioblastoma chemokine migration Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e494e14212504f93a23651eca1adbd3b
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spelling	oai:doaj.org-article:e494e14212504f93a23651eca1adbd3b2021-11-25T17:53:54ZChemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors10.3390/ijms2222121501422-00671661-6596https://doaj.org/article/e494e14212504f93a23651eca1adbd3b2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12150https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Chemoattraction is a normal and essential process, but it can also be involved in tumorigenesis. This phenomenon plays a key role in glioblastoma (GBM). The GBM tumor cells are extremely difficult to eradicate, due to their strong capacity to migrate into the brain parenchyma. Consequently, a complete resection of the tumor is rarely a possibility, and recurrence is inevitable. To overcome this problem, we proposed to exploit this behavior by using three chemoattractants: CXCL10, CCL2 and CCL11, released by a biodegradable hydrogel (GlioGel) to produce a migration of tumor cells toward a therapeutic trap. To investigate this hypothesis, the agarose drop assay was used to test the chemoattraction capacity of these three chemokines on murine F98 and human U87MG cell lines. We then studied the potency of this approach in vivo in the well-established syngeneic F98-Fischer glioma-bearing rat model using GlioGel containing different mixtures of the chemoattractants. In vitro assays resulted in an invasive cell rate 2-fold higher when chemokines were present in the environment. In vivo experiments demonstrated the capacity of these specific chemoattractants to strongly attract neoplastic glioblastoma cells. The use of this strong locomotion ability to our end is a promising avenue in the establishment of a new therapeutic approach in the treatment of primary brain tumors.Laurence DéryGabriel CharestBrigitte GuérinMohsen AkbariDavid FortinMDPI AGarticleneuro-oncologybrain tumorglioblastomachemokinemigrationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12150, p 12150 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neuro-oncology brain tumor glioblastoma chemokine migration Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	neuro-oncology brain tumor glioblastoma chemokine migration Biology (General) QH301-705.5 Chemistry QD1-999 Laurence Déry Gabriel Charest Brigitte Guérin Mohsen Akbari David Fortin Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
description	Chemoattraction is a normal and essential process, but it can also be involved in tumorigenesis. This phenomenon plays a key role in glioblastoma (GBM). The GBM tumor cells are extremely difficult to eradicate, due to their strong capacity to migrate into the brain parenchyma. Consequently, a complete resection of the tumor is rarely a possibility, and recurrence is inevitable. To overcome this problem, we proposed to exploit this behavior by using three chemoattractants: CXCL10, CCL2 and CCL11, released by a biodegradable hydrogel (GlioGel) to produce a migration of tumor cells toward a therapeutic trap. To investigate this hypothesis, the agarose drop assay was used to test the chemoattraction capacity of these three chemokines on murine F98 and human U87MG cell lines. We then studied the potency of this approach in vivo in the well-established syngeneic F98-Fischer glioma-bearing rat model using GlioGel containing different mixtures of the chemoattractants. In vitro assays resulted in an invasive cell rate 2-fold higher when chemokines were present in the environment. In vivo experiments demonstrated the capacity of these specific chemoattractants to strongly attract neoplastic glioblastoma cells. The use of this strong locomotion ability to our end is a promising avenue in the establishment of a new therapeutic approach in the treatment of primary brain tumors.
format	article
author	Laurence Déry Gabriel Charest Brigitte Guérin Mohsen Akbari David Fortin
author_facet	Laurence Déry Gabriel Charest Brigitte Guérin Mohsen Akbari David Fortin
author_sort	Laurence Déry
title	Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
title_short	Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
title_full	Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
title_fullStr	Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
title_full_unstemmed	Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
title_sort	chemoattraction of neoplastic glial cells with cxcl10, ccl2 and ccl11 as a paradigm for a promising therapeutic approach for primary brain tumors
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e494e14212504f93a23651eca1adbd3b
work_keys_str_mv	AT laurencedery chemoattractionofneoplasticglialcellswithcxcl10ccl2andccl11asaparadigmforapromisingtherapeuticapproachforprimarybraintumors AT gabrielcharest chemoattractionofneoplasticglialcellswithcxcl10ccl2andccl11asaparadigmforapromisingtherapeuticapproachforprimarybraintumors AT brigitteguerin chemoattractionofneoplasticglialcellswithcxcl10ccl2andccl11asaparadigmforapromisingtherapeuticapproachforprimarybraintumors AT mohsenakbari chemoattractionofneoplasticglialcellswithcxcl10ccl2andccl11asaparadigmforapromisingtherapeuticapproachforprimarybraintumors AT davidfortin chemoattractionofneoplasticglialcellswithcxcl10ccl2andccl11asaparadigmforapromisingtherapeuticapproachforprimarybraintumors
_version_	1718411895193468928

Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors

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