Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction

Objective: We investigated the potency of cardiac repair based on echocardiography-guided multiple percutaneous left ventricular intramyocardial injection of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) after myocardial infarction (MI).Methods: Mice with surgically induced...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Xun Wu, Di Wang, Kele Qin, Chukwuemeka Daniel Iroegbu, Kun Xiang, Yuanjing Zhou, Qing Guan, Weijie Tang, Jun Peng, Jianjun Guo, Jinfu Yang, Chengming Fan
Formato: article
Lenguaje:EN
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://doaj.org/article/e49537715dfc478986c915353aece4c8
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:e49537715dfc478986c915353aece4c8
record_format dspace
spelling oai:doaj.org-article:e49537715dfc478986c915353aece4c82021-11-04T07:22:06ZCardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction2297-055X10.3389/fcvm.2021.768873https://doaj.org/article/e49537715dfc478986c915353aece4c82021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcvm.2021.768873/fullhttps://doaj.org/toc/2297-055XObjective: We investigated the potency of cardiac repair based on echocardiography-guided multiple percutaneous left ventricular intramyocardial injection of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) after myocardial infarction (MI).Methods: Mice with surgically induced MI were randomly divided into three groups (n = 8 in each group) and subjected to echocardiography-guided percutaneous left ventricular infarcted border injection of hiPSC-CMs (single dose; 10 μl 3 × 105 cells) or repeated injections of hiPSC-CMs at post-MI weeks 1 and 2 (multiple doses). The sham group of animals underwent all surgical procedures necessary for MI induction except for ligation. Then 4 weeks after MI, heart function was measured with transthoracic echocardiography. Engraftment was evaluated through the detection of human-specific cardiac troponin T. Infarct size and collagen volume were calculated with Sirius Red/Fast Green staining. Angiogenesis was evaluated with isolectin B4 staining. Cardiac remodeling was evaluated from the cardiomyocyte minimal fiber diameter in the infarcted border zone. Apoptosis was detected via TdT-mediated dUTP Nick-End Labeling (TUNEL) staining in cardiomyocytes from the infarcted border zone.Results: No mice died after echocardiography-guided percutaneous left ventricular intramyocardial injection. hiPSC-CMs were about nine-fold higher in the multiple-dose group at week 4 compared to the single-dose group. Multiple-dose transplantation was associated with significant improvement in left ventricular function, infarct size, angiogenesis, cardiac remodeling, and cardiomyocyte apoptosis.Conclusion: Echocardiography-guided multiple percutaneous left ventricular intramyocardial injection is a feasible, satisfactory, repeatable, relatively less invasive, and effective method of delivering cell therapy. The delivery of hiPSC-CMs indicates a novel therapy for MI.Xun WuDi WangKele QinChukwuemeka Daniel IroegbuKun XiangYuanjing ZhouQing GuanWeijie TangJun PengJianjun GuoJinfu YangJinfu YangChengming FanChengming FanChengming FanFrontiers Media S.A.articlemyocardial infarctionechocardiography-guidedintramyocardial injectionstem cellstherapyDiseases of the circulatory (Cardiovascular) systemRC666-701ENFrontiers in Cardiovascular Medicine, Vol 8 (2021)
institution DOAJ
collection DOAJ
language EN
topic myocardial infarction
echocardiography-guided
intramyocardial injection
stem cells
therapy
Diseases of the circulatory (Cardiovascular) system
RC666-701
spellingShingle myocardial infarction
echocardiography-guided
intramyocardial injection
stem cells
therapy
Diseases of the circulatory (Cardiovascular) system
RC666-701
Xun Wu
Di Wang
Kele Qin
Chukwuemeka Daniel Iroegbu
Kun Xiang
Yuanjing Zhou
Qing Guan
Weijie Tang
Jun Peng
Jianjun Guo
Jinfu Yang
Jinfu Yang
Chengming Fan
Chengming Fan
Chengming Fan
Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction
description Objective: We investigated the potency of cardiac repair based on echocardiography-guided multiple percutaneous left ventricular intramyocardial injection of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) after myocardial infarction (MI).Methods: Mice with surgically induced MI were randomly divided into three groups (n = 8 in each group) and subjected to echocardiography-guided percutaneous left ventricular infarcted border injection of hiPSC-CMs (single dose; 10 μl 3 × 105 cells) or repeated injections of hiPSC-CMs at post-MI weeks 1 and 2 (multiple doses). The sham group of animals underwent all surgical procedures necessary for MI induction except for ligation. Then 4 weeks after MI, heart function was measured with transthoracic echocardiography. Engraftment was evaluated through the detection of human-specific cardiac troponin T. Infarct size and collagen volume were calculated with Sirius Red/Fast Green staining. Angiogenesis was evaluated with isolectin B4 staining. Cardiac remodeling was evaluated from the cardiomyocyte minimal fiber diameter in the infarcted border zone. Apoptosis was detected via TdT-mediated dUTP Nick-End Labeling (TUNEL) staining in cardiomyocytes from the infarcted border zone.Results: No mice died after echocardiography-guided percutaneous left ventricular intramyocardial injection. hiPSC-CMs were about nine-fold higher in the multiple-dose group at week 4 compared to the single-dose group. Multiple-dose transplantation was associated with significant improvement in left ventricular function, infarct size, angiogenesis, cardiac remodeling, and cardiomyocyte apoptosis.Conclusion: Echocardiography-guided multiple percutaneous left ventricular intramyocardial injection is a feasible, satisfactory, repeatable, relatively less invasive, and effective method of delivering cell therapy. The delivery of hiPSC-CMs indicates a novel therapy for MI.
format article
author Xun Wu
Di Wang
Kele Qin
Chukwuemeka Daniel Iroegbu
Kun Xiang
Yuanjing Zhou
Qing Guan
Weijie Tang
Jun Peng
Jianjun Guo
Jinfu Yang
Jinfu Yang
Chengming Fan
Chengming Fan
Chengming Fan
author_facet Xun Wu
Di Wang
Kele Qin
Chukwuemeka Daniel Iroegbu
Kun Xiang
Yuanjing Zhou
Qing Guan
Weijie Tang
Jun Peng
Jianjun Guo
Jinfu Yang
Jinfu Yang
Chengming Fan
Chengming Fan
Chengming Fan
author_sort Xun Wu
title Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction
title_short Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction
title_full Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction
title_fullStr Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction
title_full_unstemmed Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction
title_sort cardiac repair with echocardiography-guided multiple percutaneous left ventricular intramyocardial injection of hipsc-cms after myocardial infarction
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/e49537715dfc478986c915353aece4c8
work_keys_str_mv AT xunwu cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT diwang cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT keleqin cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT chukwuemekadanieliroegbu cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT kunxiang cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT yuanjingzhou cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT qingguan cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT weijietang cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT junpeng cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT jianjunguo cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT jinfuyang cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT jinfuyang cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT chengmingfan cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT chengmingfan cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
AT chengmingfan cardiacrepairwithechocardiographyguidedmultiplepercutaneousleftventricularintramyocardialinjectionofhipsccmsaftermyocardialinfarction
_version_ 1718445055826460672