Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus

Abstract We developed a reusable surface-amplified nanobiosensor for monitoring airborne viruses with a sub-PFU/mL level detection limit. Here, sandwich structures consisted of magnetic particles functionalized with antibodies, target viruses, and alkaline phosphatases (ALPs) were formed, and they w...

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Autores principales:	Junghyun Shin, Hyeong Rae Kim, Pan Kee Bae, Haneul Yoo, Jeongsu Kim, Yoonji Choi, Yong Beom Shin, Jungho Hwang, Seunghun Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4963c581313496dafacb7bc6b772d38
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spelling	oai:doaj.org-article:e4963c581313496dafacb7bc6b772d382021-12-02T18:51:41ZReusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus10.1038/s41598-021-96254-22045-2322https://doaj.org/article/e4963c581313496dafacb7bc6b772d382021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96254-2https://doaj.org/toc/2045-2322Abstract We developed a reusable surface-amplified nanobiosensor for monitoring airborne viruses with a sub-PFU/mL level detection limit. Here, sandwich structures consisted of magnetic particles functionalized with antibodies, target viruses, and alkaline phosphatases (ALPs) were formed, and they were magnetically concentrated on Ni patterns near an electrochemical sensor transducer. Then, the electrical signals from electrochemical markers generated by ALPs were measured with the sensor transducer, enabling highly-sensitive virus detection. The sandwich structures in the used sensor chip could be removed by applying an external magnetic field, and we could reuse the sensor transducer chip. As a proof of concepts, the repeated detection of airborne influenza virus using a single sensor chip was demonstrated with a detection limit down to a sub-PFU/mL level. Using a single reusable sensor transducer chip, the hemagglutinin (HA) of influenza A (H1N1) virus with different concentrations were measured down to 10 aM level. Importantly, our sensor chip exhibited reliable sensing signals even after more than 18 times of the repeated HA sensing measurements. Furthermore, airborne influenza viruses collected from the air could be measured down to 0.01 PFU/mL level. Interestingly, the detailed quantitative analysis of the measurement results revealed the degradation of HA proteins on the viruses after the air exposure. Considering the ultrasensitivity and reusability of our sensors, it can provide a powerful tool to help preventing epidemics by airborne pathogens in the future.Junghyun ShinHyeong Rae KimPan Kee BaeHaneul YooJeongsu KimYoonji ChoiYong Beom ShinJungho HwangSeunghun HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Junghyun Shin Hyeong Rae Kim Pan Kee Bae Haneul Yoo Jeongsu Kim Yoonji Choi Yong Beom Shin Jungho Hwang Seunghun Hong Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus
description	Abstract We developed a reusable surface-amplified nanobiosensor for monitoring airborne viruses with a sub-PFU/mL level detection limit. Here, sandwich structures consisted of magnetic particles functionalized with antibodies, target viruses, and alkaline phosphatases (ALPs) were formed, and they were magnetically concentrated on Ni patterns near an electrochemical sensor transducer. Then, the electrical signals from electrochemical markers generated by ALPs were measured with the sensor transducer, enabling highly-sensitive virus detection. The sandwich structures in the used sensor chip could be removed by applying an external magnetic field, and we could reuse the sensor transducer chip. As a proof of concepts, the repeated detection of airborne influenza virus using a single sensor chip was demonstrated with a detection limit down to a sub-PFU/mL level. Using a single reusable sensor transducer chip, the hemagglutinin (HA) of influenza A (H1N1) virus with different concentrations were measured down to 10 aM level. Importantly, our sensor chip exhibited reliable sensing signals even after more than 18 times of the repeated HA sensing measurements. Furthermore, airborne influenza viruses collected from the air could be measured down to 0.01 PFU/mL level. Interestingly, the detailed quantitative analysis of the measurement results revealed the degradation of HA proteins on the viruses after the air exposure. Considering the ultrasensitivity and reusability of our sensors, it can provide a powerful tool to help preventing epidemics by airborne pathogens in the future.
format	article
author	Junghyun Shin Hyeong Rae Kim Pan Kee Bae Haneul Yoo Jeongsu Kim Yoonji Choi Yong Beom Shin Jungho Hwang Seunghun Hong
author_facet	Junghyun Shin Hyeong Rae Kim Pan Kee Bae Haneul Yoo Jeongsu Kim Yoonji Choi Yong Beom Shin Jungho Hwang Seunghun Hong
author_sort	Junghyun Shin
title	Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus
title_short	Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus
title_full	Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus
title_fullStr	Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus
title_full_unstemmed	Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus
title_sort	reusable surface amplified nanobiosensor for the sub pfu/ml level detection of airborne virus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e4963c581313496dafacb7bc6b772d38
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Reusable surface amplified nanobiosensor for the sub PFU/mL level detection of airborne virus

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