ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS

In present work, a comparative analysis of cytostatic and cytotoxic activity of IL-4 and IFNα-induced dendritic cells (IL-4- and IFN-DCs) has been performed in healthy donors and patients with brain tumors. Cytostatic activity was evaluated, as a capacity of DCs to inhibit growth of NK-resistant HEp...

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Autores principales:	E. R. Chernykh, O. Yu. Leplina, T. V. Tyrinova, M. A. Tikhonova, V. V. Stupak, S. V. Mishinov, I. V. Pendyurin, A. A. Ostanin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	dendritic cells cytotoxic/cytostatic activity tumor cell lines brain tumors Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/e49c59ecf57c4fe5b2812ddac904fd7f
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spelling	oai:doaj.org-article:e49c59ecf57c4fe5b2812ddac904fd7f2021-11-18T08:03:37ZANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS1563-06252313-741X10.15789/1563-0625-2010-3-199-206https://doaj.org/article/e49c59ecf57c4fe5b2812ddac904fd7f2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/89https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XIn present work, a comparative analysis of cytostatic and cytotoxic activity of IL-4 and IFNα-induced dendritic cells (IL-4- and IFN-DCs) has been performed in healthy donors and patients with brain tumors. Cytostatic activity was evaluated, as a capacity of DCs to inhibit growth of NK-resistant HEp-2 and A-549 tumor cell lines, whereas cytotoxic activity was measured by assessment of HEp-2 lysis. As compared with IL4-DC, IFN-DCs from healthy donors displayed a more pronounced cytostatic effect and similar cytotoxic activity against HEp-2 cells. In the patients with brain tumors, IFN-DCs were characterized by decreased cytotoxic activity. Meanwhile, DCs from the patients with malignant gliomas virtually did not exhibit a cytotoxic potential, whereas IFN-DCs from the patients with benign brain tumors retained their cytotoxic properties. A comparative study has shown that, in contrast to healthy donors, IFN-DCs from the patients with either malignant or benign brain tumors did not inhibit HEp-2 proliferation. The loss of DC-mediated cytostatic activity was not associated with decrease of CD123+ DCs (known as cells with maximal tumor-inhibitory potential), and, most likely, it resulted from functional changes in IFN-DC populations.E. R. ChernykhO. Yu. LeplinaT. V. TyrinovaM. A. TikhonovaV. V. StupakS. V. MishinovI. V. PendyurinA. A. OstaninSPb RAACIarticledendritic cellscytotoxic/cytostatic activitytumor cell linesbrain tumorsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 3, Pp 199-206 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	dendritic cells cytotoxic/cytostatic activity tumor cell lines brain tumors Immunologic diseases. Allergy RC581-607
spellingShingle	dendritic cells cytotoxic/cytostatic activity tumor cell lines brain tumors Immunologic diseases. Allergy RC581-607 E. R. Chernykh O. Yu. Leplina T. V. Tyrinova M. A. Tikhonova V. V. Stupak S. V. Mishinov I. V. Pendyurin A. A. Ostanin ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS
description	In present work, a comparative analysis of cytostatic and cytotoxic activity of IL-4 and IFNα-induced dendritic cells (IL-4- and IFN-DCs) has been performed in healthy donors and patients with brain tumors. Cytostatic activity was evaluated, as a capacity of DCs to inhibit growth of NK-resistant HEp-2 and A-549 tumor cell lines, whereas cytotoxic activity was measured by assessment of HEp-2 lysis. As compared with IL4-DC, IFN-DCs from healthy donors displayed a more pronounced cytostatic effect and similar cytotoxic activity against HEp-2 cells. In the patients with brain tumors, IFN-DCs were characterized by decreased cytotoxic activity. Meanwhile, DCs from the patients with malignant gliomas virtually did not exhibit a cytotoxic potential, whereas IFN-DCs from the patients with benign brain tumors retained their cytotoxic properties. A comparative study has shown that, in contrast to healthy donors, IFN-DCs from the patients with either malignant or benign brain tumors did not inhibit HEp-2 proliferation. The loss of DC-mediated cytostatic activity was not associated with decrease of CD123+ DCs (known as cells with maximal tumor-inhibitory potential), and, most likely, it resulted from functional changes in IFN-DC populations.
format	article
author	E. R. Chernykh O. Yu. Leplina T. V. Tyrinova M. A. Tikhonova V. V. Stupak S. V. Mishinov I. V. Pendyurin A. A. Ostanin
author_facet	E. R. Chernykh O. Yu. Leplina T. V. Tyrinova M. A. Tikhonova V. V. Stupak S. V. Mishinov I. V. Pendyurin A. A. Ostanin
author_sort	E. R. Chernykh
title	ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS
title_short	ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS
title_full	ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS
title_fullStr	ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS
title_full_unstemmed	ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS
title_sort	anti-tumor activity of dendritic cells in healthy donors and patients with brain tumors
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/e49c59ecf57c4fe5b2812ddac904fd7f
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ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS

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