T cells recognizing a peptide contaminant undetectable by mass spectrometry.

Synthetic peptides are widely used in immunological research as epitopes to stimulate their cognate T cells. These preparations are never completely pure, but trace contaminants are commonly revealed by mass spectrometry quality controls. In an effort to characterize novel major histocompatibility c...

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Autores principales:	Vedran Brezar, Slobodan Culina, Thomas Østerbye, François Guillonneau, Giovanni Chiappetta, Yann Verdier, Joelle Vinh, F Susan Wong, Søren Buus, Roberto Mallone
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e49c69d522eb47a7bb72dbed9da90d61
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id	oai:doaj.org-article:e49c69d522eb47a7bb72dbed9da90d61
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spelling	oai:doaj.org-article:e49c69d522eb47a7bb72dbed9da90d612021-11-18T07:32:10ZT cells recognizing a peptide contaminant undetectable by mass spectrometry.1932-620310.1371/journal.pone.0028866https://doaj.org/article/e49c69d522eb47a7bb72dbed9da90d612011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194932/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Synthetic peptides are widely used in immunological research as epitopes to stimulate their cognate T cells. These preparations are never completely pure, but trace contaminants are commonly revealed by mass spectrometry quality controls. In an effort to characterize novel major histocompatibility complex (MHC) Class I-restricted β-cell epitopes in non-obese diabetic (NOD) mice, we identified islet-infiltrating CD8+ T cells recognizing a contaminating peptide. The amount of this contaminant was so small to be undetectable by direct mass spectrometry. Only after concentration by liquid chromatography, we observed a mass peak corresponding to an immunodominant islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP)(206-214) epitope described in the literature. Generation of CD8+ T-cell clones recognizing IGRP(206-214) using a novel method confirmed the identity of the contaminant, further underlining the immunodominance of IGRP(206-214). If left undetected, minute impurities in synthetic peptide preparations may thus give spurious results.Vedran BrezarSlobodan CulinaThomas ØsterbyeFrançois GuillonneauGiovanni ChiappettaYann VerdierJoelle VinhF Susan WongSøren BuusRoberto MallonePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28866 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vedran Brezar Slobodan Culina Thomas Østerbye François Guillonneau Giovanni Chiappetta Yann Verdier Joelle Vinh F Susan Wong Søren Buus Roberto Mallone T cells recognizing a peptide contaminant undetectable by mass spectrometry.
description	Synthetic peptides are widely used in immunological research as epitopes to stimulate their cognate T cells. These preparations are never completely pure, but trace contaminants are commonly revealed by mass spectrometry quality controls. In an effort to characterize novel major histocompatibility complex (MHC) Class I-restricted β-cell epitopes in non-obese diabetic (NOD) mice, we identified islet-infiltrating CD8+ T cells recognizing a contaminating peptide. The amount of this contaminant was so small to be undetectable by direct mass spectrometry. Only after concentration by liquid chromatography, we observed a mass peak corresponding to an immunodominant islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP)(206-214) epitope described in the literature. Generation of CD8+ T-cell clones recognizing IGRP(206-214) using a novel method confirmed the identity of the contaminant, further underlining the immunodominance of IGRP(206-214). If left undetected, minute impurities in synthetic peptide preparations may thus give spurious results.
format	article
author	Vedran Brezar Slobodan Culina Thomas Østerbye François Guillonneau Giovanni Chiappetta Yann Verdier Joelle Vinh F Susan Wong Søren Buus Roberto Mallone
author_facet	Vedran Brezar Slobodan Culina Thomas Østerbye François Guillonneau Giovanni Chiappetta Yann Verdier Joelle Vinh F Susan Wong Søren Buus Roberto Mallone
author_sort	Vedran Brezar
title	T cells recognizing a peptide contaminant undetectable by mass spectrometry.
title_short	T cells recognizing a peptide contaminant undetectable by mass spectrometry.
title_full	T cells recognizing a peptide contaminant undetectable by mass spectrometry.
title_fullStr	T cells recognizing a peptide contaminant undetectable by mass spectrometry.
title_full_unstemmed	T cells recognizing a peptide contaminant undetectable by mass spectrometry.
title_sort	t cells recognizing a peptide contaminant undetectable by mass spectrometry.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/e49c69d522eb47a7bb72dbed9da90d61
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T cells recognizing a peptide contaminant undetectable by mass spectrometry.

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