Complementary methods for SARS-CoV-2 diagnosis in times of material shortage

Abstract The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and ra...

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Autores principales:	Thaisa Lucas Sandri, Juliana Inoue, Johanna Geiger, Johanna-Marie Griesbaum, Constanze Heinzel, Michael Burnet, Rolf Fendel, Peter G. Kremsner, Jana Held, Andrea Kreidenweiss
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4a03982c6a84819bad572abfc336e46
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spelling	oai:doaj.org-article:e4a03982c6a84819bad572abfc336e462021-12-02T14:59:29ZComplementary methods for SARS-CoV-2 diagnosis in times of material shortage10.1038/s41598-021-91457-z2045-2322https://doaj.org/article/e4a03982c6a84819bad572abfc336e462021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91457-zhttps://doaj.org/toc/2045-2322Abstract The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2—particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources.Thaisa Lucas SandriJuliana InoueJohanna GeigerJohanna-Marie GriesbaumConstanze HeinzelMichael BurnetRolf FendelPeter G. KremsnerJana HeldAndrea KreidenweissNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thaisa Lucas Sandri Juliana Inoue Johanna Geiger Johanna-Marie Griesbaum Constanze Heinzel Michael Burnet Rolf Fendel Peter G. Kremsner Jana Held Andrea Kreidenweiss Complementary methods for SARS-CoV-2 diagnosis in times of material shortage
description	Abstract The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2—particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources.
format	article
author	Thaisa Lucas Sandri Juliana Inoue Johanna Geiger Johanna-Marie Griesbaum Constanze Heinzel Michael Burnet Rolf Fendel Peter G. Kremsner Jana Held Andrea Kreidenweiss
author_facet	Thaisa Lucas Sandri Juliana Inoue Johanna Geiger Johanna-Marie Griesbaum Constanze Heinzel Michael Burnet Rolf Fendel Peter G. Kremsner Jana Held Andrea Kreidenweiss
author_sort	Thaisa Lucas Sandri
title	Complementary methods for SARS-CoV-2 diagnosis in times of material shortage
title_short	Complementary methods for SARS-CoV-2 diagnosis in times of material shortage
title_full	Complementary methods for SARS-CoV-2 diagnosis in times of material shortage
title_fullStr	Complementary methods for SARS-CoV-2 diagnosis in times of material shortage
title_full_unstemmed	Complementary methods for SARS-CoV-2 diagnosis in times of material shortage
title_sort	complementary methods for sars-cov-2 diagnosis in times of material shortage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e4a03982c6a84819bad572abfc336e46
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Complementary methods for SARS-CoV-2 diagnosis in times of material shortage

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