Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model

Abstract Bacterial vaginosis (BV) is a gynecologic disorder characterized by a shift in cervicovaginal microbiota from Lactobacillus spp. dominance to a polymicrobial biofilm composed of diverse anaerobes. We utilized a well-characterized human three-dimensional cervical epithelial cell model in con...

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Autores principales:	Mary E. Salliss, Jason D. Maarsingh, Camryn Garza, Paweł Łaniewski, Melissa M. Herbst-Kralovetz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/e4a05f8b51b64bc1a9965de6c1f4b3d7
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spelling	oai:doaj.org-article:e4a05f8b51b64bc1a9965de6c1f4b3d72021-12-02T16:15:07ZVeillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model10.1038/s41522-021-00229-02055-5008https://doaj.org/article/e4a05f8b51b64bc1a9965de6c1f4b3d72021-07-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00229-0https://doaj.org/toc/2055-5008Abstract Bacterial vaginosis (BV) is a gynecologic disorder characterized by a shift in cervicovaginal microbiota from Lactobacillus spp. dominance to a polymicrobial biofilm composed of diverse anaerobes. We utilized a well-characterized human three-dimensional cervical epithelial cell model in conjunction with untargeted metabolomics and immunoproteomics analyses to determine the immunometabolic contribution of three members of the Veillonellaceae family: Veillonella atypica, Veillonella montpellierensis and Megasphaera micronuciformis at this site. We found that Veillonella spp. infections induced significant elevation of polyamines. M. micronuciformis infections significantly increased soluble inflammatory mediators, induced moderate levels of cell cytotoxicity, and accumulation of cell membrane lipids relative to Veillonella spp. Notably, both V. atypica and V. montpellierensis infections resulted in consumption of lactate, a key metabolite linked to gynecologic and reproductive health. Collectively our approach and data provide unique insights into the specific contributions of Veillonellaceae members to the pathogenesis of BV and women’s health.Mary E. SallissJason D. MaarsinghCamryn GarzaPaweł ŁaniewskiMelissa M. Herbst-KralovetzNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Mary E. Salliss Jason D. Maarsingh Camryn Garza Paweł Łaniewski Melissa M. Herbst-Kralovetz Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model
description	Abstract Bacterial vaginosis (BV) is a gynecologic disorder characterized by a shift in cervicovaginal microbiota from Lactobacillus spp. dominance to a polymicrobial biofilm composed of diverse anaerobes. We utilized a well-characterized human three-dimensional cervical epithelial cell model in conjunction with untargeted metabolomics and immunoproteomics analyses to determine the immunometabolic contribution of three members of the Veillonellaceae family: Veillonella atypica, Veillonella montpellierensis and Megasphaera micronuciformis at this site. We found that Veillonella spp. infections induced significant elevation of polyamines. M. micronuciformis infections significantly increased soluble inflammatory mediators, induced moderate levels of cell cytotoxicity, and accumulation of cell membrane lipids relative to Veillonella spp. Notably, both V. atypica and V. montpellierensis infections resulted in consumption of lactate, a key metabolite linked to gynecologic and reproductive health. Collectively our approach and data provide unique insights into the specific contributions of Veillonellaceae members to the pathogenesis of BV and women’s health.
format	article
author	Mary E. Salliss Jason D. Maarsingh Camryn Garza Paweł Łaniewski Melissa M. Herbst-Kralovetz
author_facet	Mary E. Salliss Jason D. Maarsingh Camryn Garza Paweł Łaniewski Melissa M. Herbst-Kralovetz
author_sort	Mary E. Salliss
title	Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model
title_short	Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model
title_full	Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model
title_fullStr	Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model
title_full_unstemmed	Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model
title_sort	veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e4a05f8b51b64bc1a9965de6c1f4b3d7
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Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model

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