Genome-wide exploration of miRNA function in mammalian muscle cell differentiation.

MiRNAs impact on the control of cell fate by regulating gene expression at the post-transcriptional level. Here, using mammalian muscle differentiation as a model and a phenotypic loss-of-function screen, we explored the function of miRNAs at the genome-wide level. We found that the depletion of a h...

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Autores principales:	Anna Polesskaya, Cindy Degerny, Guillaume Pinna, Yves Maury, Gueorgui Kratassiouk, Vincent Mouly, Nadya Morozova, Jeremie Kropp, Niels Frandsen, Annick Harel-Bellan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4a33f7173b945118e1d29c3fc3b670d
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spelling	oai:doaj.org-article:e4a33f7173b945118e1d29c3fc3b670d2021-11-18T08:58:36ZGenome-wide exploration of miRNA function in mammalian muscle cell differentiation.1932-620310.1371/journal.pone.0071927https://doaj.org/article/e4a33f7173b945118e1d29c3fc3b670d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23991007/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203MiRNAs impact on the control of cell fate by regulating gene expression at the post-transcriptional level. Here, using mammalian muscle differentiation as a model and a phenotypic loss-of-function screen, we explored the function of miRNAs at the genome-wide level. We found that the depletion of a high number of miRNAs (63) impacted on differentiation of human muscle precursors, underscoring the importance of this post-transcriptional mechanism of gene regulation. Interestingly, a comparison with miRNA expression profiles revealed that most of the hit miRNAs did not show any significant variations of expression during differentiation. These constitutively expressed miRNAs might be required for basic and/or essential cell function, or else might be regulated at the post-transcriptional level. MiRNA inhibition yielded a variety of phenotypes, reflecting the widespread miRNA involvement in differentiation. Using a functional screen (the STarS--Suppressor Target Screen--approach, i. e. concomitant knockdown of miRNAs and of candidate target proteins), we discovered miRNA protein targets that are previously uncharacterized controllers of muscle-cell terminal differentiation. Our results provide a strategy for functional annotation of the human miRnome.Anna PolesskayaCindy DegernyGuillaume PinnaYves MauryGueorgui KratassioukVincent MoulyNadya MorozovaJeremie KroppNiels FrandsenAnnick Harel-BellanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71927 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Polesskaya Cindy Degerny Guillaume Pinna Yves Maury Gueorgui Kratassiouk Vincent Mouly Nadya Morozova Jeremie Kropp Niels Frandsen Annick Harel-Bellan Genome-wide exploration of miRNA function in mammalian muscle cell differentiation.
description	MiRNAs impact on the control of cell fate by regulating gene expression at the post-transcriptional level. Here, using mammalian muscle differentiation as a model and a phenotypic loss-of-function screen, we explored the function of miRNAs at the genome-wide level. We found that the depletion of a high number of miRNAs (63) impacted on differentiation of human muscle precursors, underscoring the importance of this post-transcriptional mechanism of gene regulation. Interestingly, a comparison with miRNA expression profiles revealed that most of the hit miRNAs did not show any significant variations of expression during differentiation. These constitutively expressed miRNAs might be required for basic and/or essential cell function, or else might be regulated at the post-transcriptional level. MiRNA inhibition yielded a variety of phenotypes, reflecting the widespread miRNA involvement in differentiation. Using a functional screen (the STarS--Suppressor Target Screen--approach, i. e. concomitant knockdown of miRNAs and of candidate target proteins), we discovered miRNA protein targets that are previously uncharacterized controllers of muscle-cell terminal differentiation. Our results provide a strategy for functional annotation of the human miRnome.
format	article
author	Anna Polesskaya Cindy Degerny Guillaume Pinna Yves Maury Gueorgui Kratassiouk Vincent Mouly Nadya Morozova Jeremie Kropp Niels Frandsen Annick Harel-Bellan
author_facet	Anna Polesskaya Cindy Degerny Guillaume Pinna Yves Maury Gueorgui Kratassiouk Vincent Mouly Nadya Morozova Jeremie Kropp Niels Frandsen Annick Harel-Bellan
author_sort	Anna Polesskaya
title	Genome-wide exploration of miRNA function in mammalian muscle cell differentiation.
title_short	Genome-wide exploration of miRNA function in mammalian muscle cell differentiation.
title_full	Genome-wide exploration of miRNA function in mammalian muscle cell differentiation.
title_fullStr	Genome-wide exploration of miRNA function in mammalian muscle cell differentiation.
title_full_unstemmed	Genome-wide exploration of miRNA function in mammalian muscle cell differentiation.
title_sort	genome-wide exploration of mirna function in mammalian muscle cell differentiation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e4a33f7173b945118e1d29c3fc3b670d
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Genome-wide exploration of miRNA function in mammalian muscle cell differentiation.

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