PRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI

The CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of a glycine for a glutamic acid in position 52. Being naturally nontoxic, CRM197 is a promising adjuvant and ideal carrier protein for conjugate vaccines. Typically, production of diphtheria toxin and some...

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Autores principales:	I. V. Dukhovlinov, E. A. Fedorova, E. G. Bogomolova, O. A. Dobrovolskaya, E. N. Chernyaeva, R. I. Al-Shekhadat, A. S. Simbirtsev
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2015
Materias:	recombinant protein crm197 adjuvant conjugate vaccine diphtheria toxin escherichia coli Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/e4a638dcdd2c4d69b05b095dff972fac
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spelling	oai:doaj.org-article:e4a638dcdd2c4d69b05b095dff972fac2021-11-22T07:09:47ZPRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI2220-76192313-739810.15789/2220-7619-2015-1-37-44https://doaj.org/article/e4a638dcdd2c4d69b05b095dff972fac2015-04-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/272https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of a glycine for a glutamic acid in position 52. Being naturally nontoxic, CRM197 is a promising adjuvant and ideal carrier protein for conjugate vaccines. Typically, production of diphtheria toxin and some of the non-toxic proteins are carried out by Corynebacterium diphtheriae. Production of recombinant protein CRM197 in Escherichia coli is advantageous. It is simple, cheap and permits production of the target protein in a short time using a non-pathogenic microorganism. In this study patented high-yield-producing E. coli strain was used. As a part of the study the following steps were taken: protocol adjustment for induction of crm197 gene, production and purification of recombinant CRM197 by ion-exchange, hydrophobic and gel-filtration chromatography. The purity of the final preparation reached 97%.I. V. DukhovlinovE. A. FedorovaE. G. BogomolovaO. A. DobrovolskayaE. N. ChernyaevaR. I. Al-ShekhadatA. S. SimbirtsevSankt-Peterburg : NIIÈM imeni Pasteraarticlerecombinant proteincrm197adjuvantconjugate vaccinediphtheria toxinescherichia coliInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 5, Iss 1, Pp 37-44 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	recombinant protein crm197 adjuvant conjugate vaccine diphtheria toxin escherichia coli Infectious and parasitic diseases RC109-216
spellingShingle	recombinant protein crm197 adjuvant conjugate vaccine diphtheria toxin escherichia coli Infectious and parasitic diseases RC109-216 I. V. Dukhovlinov E. A. Fedorova E. G. Bogomolova O. A. Dobrovolskaya E. N. Chernyaeva R. I. Al-Shekhadat A. S. Simbirtsev PRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI
description	The CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of a glycine for a glutamic acid in position 52. Being naturally nontoxic, CRM197 is a promising adjuvant and ideal carrier protein for conjugate vaccines. Typically, production of diphtheria toxin and some of the non-toxic proteins are carried out by Corynebacterium diphtheriae. Production of recombinant protein CRM197 in Escherichia coli is advantageous. It is simple, cheap and permits production of the target protein in a short time using a non-pathogenic microorganism. In this study patented high-yield-producing E. coli strain was used. As a part of the study the following steps were taken: protocol adjustment for induction of crm197 gene, production and purification of recombinant CRM197 by ion-exchange, hydrophobic and gel-filtration chromatography. The purity of the final preparation reached 97%.
format	article
author	I. V. Dukhovlinov E. A. Fedorova E. G. Bogomolova O. A. Dobrovolskaya E. N. Chernyaeva R. I. Al-Shekhadat A. S. Simbirtsev
author_facet	I. V. Dukhovlinov E. A. Fedorova E. G. Bogomolova O. A. Dobrovolskaya E. N. Chernyaeva R. I. Al-Shekhadat A. S. Simbirtsev
author_sort	I. V. Dukhovlinov
title	PRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI
title_short	PRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI
title_full	PRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI
title_fullStr	PRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI
title_full_unstemmed	PRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI
title_sort	production of recombinant protein crm197 in escherichia coli
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2015
url	https://doaj.org/article/e4a638dcdd2c4d69b05b095dff972fac
work_keys_str_mv	AT ivdukhovlinov productionofrecombinantproteincrm197inescherichiacoli AT eafedorova productionofrecombinantproteincrm197inescherichiacoli AT egbogomolova productionofrecombinantproteincrm197inescherichiacoli AT oadobrovolskaya productionofrecombinantproteincrm197inescherichiacoli AT enchernyaeva productionofrecombinantproteincrm197inescherichiacoli AT rialshekhadat productionofrecombinantproteincrm197inescherichiacoli AT assimbirtsev productionofrecombinantproteincrm197inescherichiacoli
_version_	1718418076460908544

PRODUCTION OF RECOMBINANT PROTEIN CRM197 IN ESCHERICHIA COLI

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