Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins

Abstract Structure of interphase cell nuclei remains dynamic and can undergo various changes of shape and organisation, in health and disease. The double-membraned envelope that separates nuclear genetic material from the rest of the cell frequently includes deep, branching tubular invaginations tha...

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Autores principales:	Marek M. Drozdz, Haibo Jiang, Lior Pytowski, Chris Grovenor, David J. Vaux
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4a6e29d265a4069ba5671fb873e9eed
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spelling	oai:doaj.org-article:e4a6e29d265a4069ba5671fb873e9eed2021-12-02T11:52:40ZFormation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins10.1038/s41598-017-07614-w2045-2322https://doaj.org/article/e4a6e29d265a4069ba5671fb873e9eed2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07614-whttps://doaj.org/toc/2045-2322Abstract Structure of interphase cell nuclei remains dynamic and can undergo various changes of shape and organisation, in health and disease. The double-membraned envelope that separates nuclear genetic material from the rest of the cell frequently includes deep, branching tubular invaginations that form a dynamic nucleoplasmic reticulum (NR). This study addresses mechanisms by which NR can form in interphase nuclei. We present a combination of Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) approach and light microscopy techniques to follow formation of NR by using pulse-chase experiments to examine protein and lipid delivery to nascent NR in cultured cells. Lamina protein incorporation was assessed using precursor accumulation (for lamin A) or a MAPLE3 photoconvertible tag (for lamin B1) and membrane phospholipid incorporation using stable isotope labelling with deuterated precursors followed by high resolution NanoSIMS. In all three cases, nascent molecules were selectively incorporated into newly forming NR tubules; thus strongly suggesting that NR formation is a regulated process involving a focal assembly machine, rather than simple physical perturbation of a pre-existing nuclear envelope.Marek M. DrozdzHaibo JiangLior PytowskiChris GrovenorDavid J. VauxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marek M. Drozdz Haibo Jiang Lior Pytowski Chris Grovenor David J. Vaux Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins
description	Abstract Structure of interphase cell nuclei remains dynamic and can undergo various changes of shape and organisation, in health and disease. The double-membraned envelope that separates nuclear genetic material from the rest of the cell frequently includes deep, branching tubular invaginations that form a dynamic nucleoplasmic reticulum (NR). This study addresses mechanisms by which NR can form in interphase nuclei. We present a combination of Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) approach and light microscopy techniques to follow formation of NR by using pulse-chase experiments to examine protein and lipid delivery to nascent NR in cultured cells. Lamina protein incorporation was assessed using precursor accumulation (for lamin A) or a MAPLE3 photoconvertible tag (for lamin B1) and membrane phospholipid incorporation using stable isotope labelling with deuterated precursors followed by high resolution NanoSIMS. In all three cases, nascent molecules were selectively incorporated into newly forming NR tubules; thus strongly suggesting that NR formation is a regulated process involving a focal assembly machine, rather than simple physical perturbation of a pre-existing nuclear envelope.
format	article
author	Marek M. Drozdz Haibo Jiang Lior Pytowski Chris Grovenor David J. Vaux
author_facet	Marek M. Drozdz Haibo Jiang Lior Pytowski Chris Grovenor David J. Vaux
author_sort	Marek M. Drozdz
title	Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins
title_short	Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins
title_full	Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins
title_fullStr	Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins
title_full_unstemmed	Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins
title_sort	formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e4a6e29d265a4069ba5671fb873e9eed
work_keys_str_mv	AT marekmdrozdz formationofanucleoplasmicreticulumrequiresdenovoassemblyofnascentphospholipidsandshowspreferentialincorporationofnascentlamins AT haibojiang formationofanucleoplasmicreticulumrequiresdenovoassemblyofnascentphospholipidsandshowspreferentialincorporationofnascentlamins AT liorpytowski formationofanucleoplasmicreticulumrequiresdenovoassemblyofnascentphospholipidsandshowspreferentialincorporationofnascentlamins AT chrisgrovenor formationofanucleoplasmicreticulumrequiresdenovoassemblyofnascentphospholipidsandshowspreferentialincorporationofnascentlamins AT davidjvaux formationofanucleoplasmicreticulumrequiresdenovoassemblyofnascentphospholipidsandshowspreferentialincorporationofnascentlamins
_version_	1718395016871673856

Formation of a nucleoplasmic reticulum requires de novo assembly of nascent phospholipids and shows preferential incorporation of nascent lamins

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