Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy

Abstract We show that using infiltration of ZnO metal oxide can be useful for high resolution imaging of biological samples in electron and X-ray microscopy. The method is compatible with standard fixation techniques that leave the sample dry, such as finishing with super critical CO2 drying, or sim...

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Autores principales:	L. E. Ocola, V. Sampathkumar, N. Kasthuri, R. P. Winarski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4a9f6b228b743e98b6703549501756a
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spelling	oai:doaj.org-article:e4a9f6b228b743e98b6703549501756a2021-12-02T16:08:07ZContrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy10.1038/s41598-017-05690-62045-2322https://doaj.org/article/e4a9f6b228b743e98b6703549501756a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05690-6https://doaj.org/toc/2045-2322Abstract We show that using infiltration of ZnO metal oxide can be useful for high resolution imaging of biological samples in electron and X-ray microscopy. The method is compatible with standard fixation techniques that leave the sample dry, such as finishing with super critical CO2 drying, or simple vacuum drying up to 95 °C. We demonstrate this technique can be applied on tooth and brain tissue samples. We also show that high resolution X-ray tomography can be performed on biological systems using Zn K edge (1s) absorption to enhance internal structures, and obtained the first nanoscale 10 KeV X-ray absorption images of the interior regions of a tooth.L. E. OcolaV. SampathkumarN. KasthuriR. P. WinarskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q L. E. Ocola V. Sampathkumar N. Kasthuri R. P. Winarski Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy
description	Abstract We show that using infiltration of ZnO metal oxide can be useful for high resolution imaging of biological samples in electron and X-ray microscopy. The method is compatible with standard fixation techniques that leave the sample dry, such as finishing with super critical CO2 drying, or simple vacuum drying up to 95 °C. We demonstrate this technique can be applied on tooth and brain tissue samples. We also show that high resolution X-ray tomography can be performed on biological systems using Zn K edge (1s) absorption to enhance internal structures, and obtained the first nanoscale 10 KeV X-ray absorption images of the interior regions of a tooth.
format	article
author	L. E. Ocola V. Sampathkumar N. Kasthuri R. P. Winarski
author_facet	L. E. Ocola V. Sampathkumar N. Kasthuri R. P. Winarski
author_sort	L. E. Ocola
title	Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy
title_short	Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy
title_full	Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy
title_fullStr	Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy
title_full_unstemmed	Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy
title_sort	contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and x-ray nanoscale microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e4a9f6b228b743e98b6703549501756a
work_keys_str_mv	AT leocola contrastenhancementofbiologicalnanoporousmaterialswithzincoxideinfiltrationforelectronandxraynanoscalemicroscopy AT vsampathkumar contrastenhancementofbiologicalnanoporousmaterialswithzincoxideinfiltrationforelectronandxraynanoscalemicroscopy AT nkasthuri contrastenhancementofbiologicalnanoporousmaterialswithzincoxideinfiltrationforelectronandxraynanoscalemicroscopy AT rpwinarski contrastenhancementofbiologicalnanoporousmaterialswithzincoxideinfiltrationforelectronandxraynanoscalemicroscopy
_version_	1718384626223808512

Contrast enhancement of biological nanoporous materials with zinc oxide infiltration for electron and X-ray nanoscale microscopy

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