Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells

Abstract Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effec...

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Autores principales:	Ji-Hun Kang, Hyun Joo Lee, Ok-Hyeon Kim, Yong Ju Yun, Young-Jin Seo, Hyun Jung Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4aaa291390f49fab3558b34ab2d1f61
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spelling	oai:doaj.org-article:e4aaa291390f49fab3558b34ab2d1f612021-12-02T17:47:23ZBiomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells10.1038/s41598-021-91117-22045-2322https://doaj.org/article/e4aaa291390f49fab3558b34ab2d1f612021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91117-2https://doaj.org/toc/2045-2322Abstract Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effects. However, little is known about the effects of biomechanical forces on the activation of DCs. Accordingly, here we fabricated a microfluidics system to assess how biomechanical forces affect the migration and activity of DCs during inflammation. Based on the structure of edema, we proposed and experimentally analyzed a novel concept for a microchip model that mimicked such vascular architecture. The intensity of shear stress generated in our engineered chip was found as 0.2–0.6 dyne/cm2 by computational simulation; this value corresponded to inflammation in tissues. In this platform, the directness and FMIs of DCs were significantly increased, whereas the migration velocity of DCs was not altered by shear stress, indicating that mechanical stimuli influenced DC migration. Moreover, DCs with shear stress showed increased expression of the DC activation markers MHC class I and CD86 compared with DCs under static conditions. Taken together, these data suggest that the biomechanical forces are important to regulate the migration and activity of DCs.Ji-Hun KangHyun Joo LeeOk-Hyeon KimYong Ju YunYoung-Jin SeoHyun Jung LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ji-Hun Kang Hyun Joo Lee Ok-Hyeon Kim Yong Ju Yun Young-Jin Seo Hyun Jung Lee Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells
description	Abstract Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effects. However, little is known about the effects of biomechanical forces on the activation of DCs. Accordingly, here we fabricated a microfluidics system to assess how biomechanical forces affect the migration and activity of DCs during inflammation. Based on the structure of edema, we proposed and experimentally analyzed a novel concept for a microchip model that mimicked such vascular architecture. The intensity of shear stress generated in our engineered chip was found as 0.2–0.6 dyne/cm2 by computational simulation; this value corresponded to inflammation in tissues. In this platform, the directness and FMIs of DCs were significantly increased, whereas the migration velocity of DCs was not altered by shear stress, indicating that mechanical stimuli influenced DC migration. Moreover, DCs with shear stress showed increased expression of the DC activation markers MHC class I and CD86 compared with DCs under static conditions. Taken together, these data suggest that the biomechanical forces are important to regulate the migration and activity of DCs.
format	article
author	Ji-Hun Kang Hyun Joo Lee Ok-Hyeon Kim Yong Ju Yun Young-Jin Seo Hyun Jung Lee
author_facet	Ji-Hun Kang Hyun Joo Lee Ok-Hyeon Kim Yong Ju Yun Young-Jin Seo Hyun Jung Lee
author_sort	Ji-Hun Kang
title	Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells
title_short	Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells
title_full	Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells
title_fullStr	Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells
title_full_unstemmed	Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells
title_sort	biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e4aaa291390f49fab3558b34ab2d1f61
work_keys_str_mv	AT jihunkang biomechanicalforcesenhancedirectedmigrationandactivationofbonemarrowderiveddendriticcells AT hyunjoolee biomechanicalforcesenhancedirectedmigrationandactivationofbonemarrowderiveddendriticcells AT okhyeonkim biomechanicalforcesenhancedirectedmigrationandactivationofbonemarrowderiveddendriticcells AT yongjuyun biomechanicalforcesenhancedirectedmigrationandactivationofbonemarrowderiveddendriticcells AT youngjinseo biomechanicalforcesenhancedirectedmigrationandactivationofbonemarrowderiveddendriticcells AT hyunjunglee biomechanicalforcesenhancedirectedmigrationandactivationofbonemarrowderiveddendriticcells
_version_	1718379500791660544

Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells

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