Self-agglomerated collagen patterns govern cell behaviour

Abstract Reciprocity between cells and their surrounding extracellular matrix is one of the main drivers for cellular function and, in turn, matrix maintenance and remodelling. Unravelling how cells respond to their environment is key in understanding mechanisms of health and disease. In all these e...

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Autores principales:	Aysegul Dede Eren, E. Deniz Eren, Twan J. S. Wilting, Jan de Boer, Hanneke Gelderblom, Jasper Foolen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e4aaf63e4bd4430180ca04d939ac7a24
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spelling	oai:doaj.org-article:e4aaf63e4bd4430180ca04d939ac7a242021-12-02T14:12:43ZSelf-agglomerated collagen patterns govern cell behaviour10.1038/s41598-021-81054-52045-2322https://doaj.org/article/e4aaf63e4bd4430180ca04d939ac7a242021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81054-5https://doaj.org/toc/2045-2322Abstract Reciprocity between cells and their surrounding extracellular matrix is one of the main drivers for cellular function and, in turn, matrix maintenance and remodelling. Unravelling how cells respond to their environment is key in understanding mechanisms of health and disease. In all these examples, matrix anisotropy is an important element, since it can alter the cell shape and fate. In this work, the objective is to develop and exploit easy-to-produce platforms that can be used to study the cellular response to natural proteins assembled into diverse topographical cues. We demonstrate a robust and simple approach to form collagen substrates with different topographies by evaporating droplets of a collagen solution. Upon evaporation of the collagen solution, a stain of collagen is left behind, composed of three regions with a distinct pattern: an isotropic region, a concentric ring pattern, and a radially oriented region. The formation and size of these regions can be controlled by the evaporation rate of the droplet and initial collagen concentration. The patterns form topographical cues inducing a pattern-specific cell (tenocyte) morphology, density, and proliferation. Rapid and cost-effective production of different self-agglomerated collagen topographies and their interfaces enables further study of the cell shape-phenotype relationship in vitro. Substrate topography and in analogy tissue architecture remains a cue that can and will be used to steer and understand cell function in vitro, which in turn can be applied in vivo, e.g. in optimizing tissue engineering applications.Aysegul Dede ErenE. Deniz ErenTwan J. S. WiltingJan de BoerHanneke GelderblomJasper FoolenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aysegul Dede Eren E. Deniz Eren Twan J. S. Wilting Jan de Boer Hanneke Gelderblom Jasper Foolen Self-agglomerated collagen patterns govern cell behaviour
description	Abstract Reciprocity between cells and their surrounding extracellular matrix is one of the main drivers for cellular function and, in turn, matrix maintenance and remodelling. Unravelling how cells respond to their environment is key in understanding mechanisms of health and disease. In all these examples, matrix anisotropy is an important element, since it can alter the cell shape and fate. In this work, the objective is to develop and exploit easy-to-produce platforms that can be used to study the cellular response to natural proteins assembled into diverse topographical cues. We demonstrate a robust and simple approach to form collagen substrates with different topographies by evaporating droplets of a collagen solution. Upon evaporation of the collagen solution, a stain of collagen is left behind, composed of three regions with a distinct pattern: an isotropic region, a concentric ring pattern, and a radially oriented region. The formation and size of these regions can be controlled by the evaporation rate of the droplet and initial collagen concentration. The patterns form topographical cues inducing a pattern-specific cell (tenocyte) morphology, density, and proliferation. Rapid and cost-effective production of different self-agglomerated collagen topographies and their interfaces enables further study of the cell shape-phenotype relationship in vitro. Substrate topography and in analogy tissue architecture remains a cue that can and will be used to steer and understand cell function in vitro, which in turn can be applied in vivo, e.g. in optimizing tissue engineering applications.
format	article
author	Aysegul Dede Eren E. Deniz Eren Twan J. S. Wilting Jan de Boer Hanneke Gelderblom Jasper Foolen
author_facet	Aysegul Dede Eren E. Deniz Eren Twan J. S. Wilting Jan de Boer Hanneke Gelderblom Jasper Foolen
author_sort	Aysegul Dede Eren
title	Self-agglomerated collagen patterns govern cell behaviour
title_short	Self-agglomerated collagen patterns govern cell behaviour
title_full	Self-agglomerated collagen patterns govern cell behaviour
title_fullStr	Self-agglomerated collagen patterns govern cell behaviour
title_full_unstemmed	Self-agglomerated collagen patterns govern cell behaviour
title_sort	self-agglomerated collagen patterns govern cell behaviour
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e4aaf63e4bd4430180ca04d939ac7a24
work_keys_str_mv	AT ayseguldedeeren selfagglomeratedcollagenpatternsgoverncellbehaviour AT edenizeren selfagglomeratedcollagenpatternsgoverncellbehaviour AT twanjswilting selfagglomeratedcollagenpatternsgoverncellbehaviour AT jandeboer selfagglomeratedcollagenpatternsgoverncellbehaviour AT hannekegelderblom selfagglomeratedcollagenpatternsgoverncellbehaviour AT jasperfoolen selfagglomeratedcollagenpatternsgoverncellbehaviour
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Self-agglomerated collagen patterns govern cell behaviour

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