Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis

Aims: Circulating amino acid (AA) abnormalities serve as predictors of adverse outcomes in patients with heart failure (HF). However, the role of the gut microbiota in AA disturbances remains unknown. Thus, we investigated gut microbial functions and their associations with AA metabolic alterations...

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Autores principales:	Tomohiro Hayashi, Tomoya Yamashita, Tomoya Takahashi, Tokiko Tabata, Hikaru Watanabe, Yasuhiro Gotoh, Masakazu Shinohara, Kenjiro Kami, Hidekazu Tanaka, Kensuke Matsumoto, Tetsuya Hayashi, Takuji Yamada, Ken-ichi Hirata
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	heart failure gut microbiota amino acid (AA) metabolism metagenomic analysis Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/e4bce4d3ccba4999961682680d432511
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spelling	oai:doaj.org-article:e4bce4d3ccba4999961682680d4325112021-12-01T13:35:46ZUncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis2297-055X10.3389/fcvm.2021.789325https://doaj.org/article/e4bce4d3ccba4999961682680d4325112021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcvm.2021.789325/fullhttps://doaj.org/toc/2297-055XAims: Circulating amino acid (AA) abnormalities serve as predictors of adverse outcomes in patients with heart failure (HF). However, the role of the gut microbiota in AA disturbances remains unknown. Thus, we investigated gut microbial functions and their associations with AA metabolic alterations in patients with HF.Methods and Results: We performed whole-genome shotgun sequencing of fecal samples and mass spectrometry-based profiling of AAs in patients with compensated HF. Plasma levels of total essential AAs (EAAs) and histidine were significantly lower in patients with HF than in control subjects. HF patients also displayed increased and decreased abundance of gut microbial genes involved in the degradation and biosynthesis, respectively, of EAAs, including branched-chain AAs (BCAAs) and histidine. Importantly, a significant positive correlation was observed between the abundance of microbial genes involved in BCAA biosynthesis and plasma BCAA levels in patients with HF, but not in controls. Moreover, network analysis revealed that the depletion of Eubacterium and Prevotella, which harbor genes for BCAA and histidine biosynthesis, contributed to decreased abundance of microbial genes involved in the biosynthesis of those EAAs in patients with HF.Conclusions: The present study demonstrated the relationship between gut microbiota and AA metabolic disturbances in patients with HF.Tomohiro HayashiTomohiro HayashiTomoya YamashitaTomoya TakahashiTokiko TabataHikaru WatanabeYasuhiro GotohMasakazu ShinoharaMasakazu ShinoharaKenjiro KamiHidekazu TanakaKensuke MatsumotoTetsuya HayashiTakuji YamadaKen-ichi HirataFrontiers Media S.A.articleheart failuregut microbiotaamino acid (AA)metabolismmetagenomic analysisDiseases of the circulatory (Cardiovascular) systemRC666-701ENFrontiers in Cardiovascular Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	heart failure gut microbiota amino acid (AA) metabolism metagenomic analysis Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	heart failure gut microbiota amino acid (AA) metabolism metagenomic analysis Diseases of the circulatory (Cardiovascular) system RC666-701 Tomohiro Hayashi Tomohiro Hayashi Tomoya Yamashita Tomoya Takahashi Tokiko Tabata Hikaru Watanabe Yasuhiro Gotoh Masakazu Shinohara Masakazu Shinohara Kenjiro Kami Hidekazu Tanaka Kensuke Matsumoto Tetsuya Hayashi Takuji Yamada Ken-ichi Hirata Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis
description	Aims: Circulating amino acid (AA) abnormalities serve as predictors of adverse outcomes in patients with heart failure (HF). However, the role of the gut microbiota in AA disturbances remains unknown. Thus, we investigated gut microbial functions and their associations with AA metabolic alterations in patients with HF.Methods and Results: We performed whole-genome shotgun sequencing of fecal samples and mass spectrometry-based profiling of AAs in patients with compensated HF. Plasma levels of total essential AAs (EAAs) and histidine were significantly lower in patients with HF than in control subjects. HF patients also displayed increased and decreased abundance of gut microbial genes involved in the degradation and biosynthesis, respectively, of EAAs, including branched-chain AAs (BCAAs) and histidine. Importantly, a significant positive correlation was observed between the abundance of microbial genes involved in BCAA biosynthesis and plasma BCAA levels in patients with HF, but not in controls. Moreover, network analysis revealed that the depletion of Eubacterium and Prevotella, which harbor genes for BCAA and histidine biosynthesis, contributed to decreased abundance of microbial genes involved in the biosynthesis of those EAAs in patients with HF.Conclusions: The present study demonstrated the relationship between gut microbiota and AA metabolic disturbances in patients with HF.
format	article
author	Tomohiro Hayashi Tomohiro Hayashi Tomoya Yamashita Tomoya Takahashi Tokiko Tabata Hikaru Watanabe Yasuhiro Gotoh Masakazu Shinohara Masakazu Shinohara Kenjiro Kami Hidekazu Tanaka Kensuke Matsumoto Tetsuya Hayashi Takuji Yamada Ken-ichi Hirata
author_facet	Tomohiro Hayashi Tomohiro Hayashi Tomoya Yamashita Tomoya Takahashi Tokiko Tabata Hikaru Watanabe Yasuhiro Gotoh Masakazu Shinohara Masakazu Shinohara Kenjiro Kami Hidekazu Tanaka Kensuke Matsumoto Tetsuya Hayashi Takuji Yamada Ken-ichi Hirata
author_sort	Tomohiro Hayashi
title	Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis
title_short	Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis
title_full	Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis
title_fullStr	Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis
title_full_unstemmed	Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis
title_sort	uncovering the role of gut microbiota in amino acid metabolic disturbances in heart failure through metagenomic analysis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e4bce4d3ccba4999961682680d432511
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Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis

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