Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients

Najmiatul Masykura,1 Jamal Zaini,2 Elisna Syahruddin,2 Sita Laksmi Andarini,2 Achmad Hudoyo,2 Refniwita Yasril,2 Asep Ridwanuloh,3 Heriawaty Hidajat,4 Fariz Nurwidya,2 Ahmad Utomo1,51Cancer Diagnostic Research, Stem-cell and Cancer Institute, Jakarta, Indonesia; 2Department of Pulmonology and Respir...

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Autores principales:	Masykura N, Zaini J, Syahruddin E, Andarini SL, Hudoyo A, Yasril R, Ridwanuloh A, Hidajat H, Nurwidya F, Utomo A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Lung cancer Indonesia K-RAS mutation smoking Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e4c2d86f69024397bb55a8263aaf953e
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spelling	oai:doaj.org-article:e4c2d86f69024397bb55a8263aaf953e2021-12-02T05:42:19ZImpact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients1179-2728https://doaj.org/article/e4c2d86f69024397bb55a8263aaf953e2019-06-01T00:00:00Zhttps://www.dovepress.com/impact-of-smoking-on-frequency-and-spectrum-of-k-ras-and-egfr-mutation-peer-reviewed-article-LCTThttps://doaj.org/toc/1179-2728Najmiatul Masykura,1 Jamal Zaini,2 Elisna Syahruddin,2 Sita Laksmi Andarini,2 Achmad Hudoyo,2 Refniwita Yasril,2 Asep Ridwanuloh,3 Heriawaty Hidajat,4 Fariz Nurwidya,2 Ahmad Utomo1,51Cancer Diagnostic Research, Stem-cell and Cancer Institute, Jakarta, Indonesia; 2Department of Pulmonology and Respiratory Medicine Faculty of Medicine, Universitas Indonesia and Persahabatan Hospital, Jakarta, Indonesia; 3Research Center for Biotechnology, Indonesian Institute of Sciences, Bogor, Indonesia; 4Anatomic Pathology Laboratory, Persahabatan Hospital, Jakarta, Indonesia; 5Molecular Genetic Testing Services, Kalbe Genomics Laboratory, Jakarta, IndonesiaBackground: Indonesia has the highest cigarette consumption in the world. We explored the clinical impact of smoking on the prevalence of EGFR and K-RAS mutations and survival in this prospective study.Methods: 143 treatment naive lung cancer patients were recruited from Persahabatan Hospital, a national tertiary hospital. DNA from cytological specimens had been extracted and genotyped for both EGFR and K-RAS mutations using a combination of PCR high resolution melting, restriction fragment length polymorphism (RFLP) and direct DNA sequencing.Results: EGFR mutation frequency in never smokers (NS) and ever smokers (ES) were 75% and 56% (p = 0.0401), respectively. In this cohort, the overall K-RAS mutation rate was 7%. Neither gender nor smoking history were associated with K-RAS mutation significantly. However, K-RAS transversion mutations were more common in male ES than transition mutations. Smoking history did not affect EGFR and K-RAS mutation frequencies in women. Concurrent EGFR/K-RAS mutation rate was 2.8% (4 of 143 patients). Four out of 91 EGFR mutation positive patients (4.4%) had simultaneous K-RAS mutation.Conclusions: In region where cigarette consumption is prevalent, smoking history affected frequencies of EGFR and K-RAS mutations, mainly in males.Keywords: lung cancer, Indonesia, K-RAS mutation, smokingMasykura NZaini JSyahruddin EAndarini SLHudoyo AYasril RRidwanuloh AHidajat HNurwidya FUtomo ADove Medical PressarticleLung cancerIndonesiaK-RAS mutationsmokingNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENLung Cancer: Targets and Therapy, Vol Volume 10, Pp 57-66 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lung cancer Indonesia K-RAS mutation smoking Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Lung cancer Indonesia K-RAS mutation smoking Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Masykura N Zaini J Syahruddin E Andarini SL Hudoyo A Yasril R Ridwanuloh A Hidajat H Nurwidya F Utomo A Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients
description	Najmiatul Masykura,1 Jamal Zaini,2 Elisna Syahruddin,2 Sita Laksmi Andarini,2 Achmad Hudoyo,2 Refniwita Yasril,2 Asep Ridwanuloh,3 Heriawaty Hidajat,4 Fariz Nurwidya,2 Ahmad Utomo1,51Cancer Diagnostic Research, Stem-cell and Cancer Institute, Jakarta, Indonesia; 2Department of Pulmonology and Respiratory Medicine Faculty of Medicine, Universitas Indonesia and Persahabatan Hospital, Jakarta, Indonesia; 3Research Center for Biotechnology, Indonesian Institute of Sciences, Bogor, Indonesia; 4Anatomic Pathology Laboratory, Persahabatan Hospital, Jakarta, Indonesia; 5Molecular Genetic Testing Services, Kalbe Genomics Laboratory, Jakarta, IndonesiaBackground: Indonesia has the highest cigarette consumption in the world. We explored the clinical impact of smoking on the prevalence of EGFR and K-RAS mutations and survival in this prospective study.Methods: 143 treatment naive lung cancer patients were recruited from Persahabatan Hospital, a national tertiary hospital. DNA from cytological specimens had been extracted and genotyped for both EGFR and K-RAS mutations using a combination of PCR high resolution melting, restriction fragment length polymorphism (RFLP) and direct DNA sequencing.Results: EGFR mutation frequency in never smokers (NS) and ever smokers (ES) were 75% and 56% (p = 0.0401), respectively. In this cohort, the overall K-RAS mutation rate was 7%. Neither gender nor smoking history were associated with K-RAS mutation significantly. However, K-RAS transversion mutations were more common in male ES than transition mutations. Smoking history did not affect EGFR and K-RAS mutation frequencies in women. Concurrent EGFR/K-RAS mutation rate was 2.8% (4 of 143 patients). Four out of 91 EGFR mutation positive patients (4.4%) had simultaneous K-RAS mutation.Conclusions: In region where cigarette consumption is prevalent, smoking history affected frequencies of EGFR and K-RAS mutations, mainly in males.Keywords: lung cancer, Indonesia, K-RAS mutation, smoking
format	article
author	Masykura N Zaini J Syahruddin E Andarini SL Hudoyo A Yasril R Ridwanuloh A Hidajat H Nurwidya F Utomo A
author_facet	Masykura N Zaini J Syahruddin E Andarini SL Hudoyo A Yasril R Ridwanuloh A Hidajat H Nurwidya F Utomo A
author_sort	Masykura N
title	Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients
title_short	Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients
title_full	Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients
title_fullStr	Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients
title_full_unstemmed	Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients
title_sort	impact of smoking on frequency and spectrum of k-ras and egfr mutations in treatment naive indonesian lung cancer patients
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/e4c2d86f69024397bb55a8263aaf953e
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Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients

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