Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities

Vasudeva Reddy Netala,1 Murali Satyanarayana Bethu,2,3 Bobbu Pushpalatha,1 Vijaya Bhaskar Baki,4 Sani Aishwarya,1 J Venkateswara Rao,2 Vijaya Tartte5 1Department of Biotechnology, Sri Venkateswara University, Tirupati, 2Biology Division, Indian Institute of Chemical Technology, Hyderabad, 3Academy...

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Autores principales:	Netala VR, Bethu MS, Pushpalatha B, Baki VB, Aishwarya S, Rao JV, Tartte V
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Silver nanoparticles Pestalotiopsis microspora Antioxidant activity Anticancer activity Human ovarian carcinoma SKOV3 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e4c6091f8a994d2f9ce5d928258efb27
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spelling	oai:doaj.org-article:e4c6091f8a994d2f9ce5d928258efb272021-12-02T03:05:14ZBiogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities1178-2013https://doaj.org/article/e4c6091f8a994d2f9ce5d928258efb272016-10-01T00:00:00Zhttps://www.dovepress.com/biogenesis-of-silver-nanoparticles-using-endophytic-fungus-pestalotiop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Vasudeva Reddy Netala,1 Murali Satyanarayana Bethu,2,3 Bobbu Pushpalatha,1 Vijaya Bhaskar Baki,4 Sani Aishwarya,1 J Venkateswara Rao,2 Vijaya Tartte5 1Department of Biotechnology, Sri Venkateswara University, Tirupati, 2Biology Division, Indian Institute of Chemical Technology, Hyderabad, 3Academy of Scientific and Innovative Research, New Delhi, 4Department of Bioinformatics, 5Department of Botany, Sri Venkateswara University, Tirupati, India Abstract: An endophytic fungal strain isolated from the leaves of Gymnema sylvestre was identified as Pestalotiopsis microspora VJ1/VS1 based on nucleotide sequencing of internal transcribed spacer region (ITS 1-5.8S-ITS 2) of 18S rRNA gene (NCBI accession number KX213894). In this study, an efficient and ecofriendly approach has been reported for the synthesis of silver nanoparticles (AgNPs) using aqueous culture filtrate of P. microspora. Ultraviolet-visible analysis confirmed the synthesis of AgNPs by showing characteristic absorption peak at 435 nm. Fourier transform infrared spectroscopy analysis revealed the presence of phenolic compounds and proteins in the fungal filtrate, which are plausibly involved in the biosynthesis and capping of AgNPs. Transmission electron microscopy (TEM) showed that the AgNPs were spherical in shape of 2–10 nm in size. Selected area electron diffraction and X-ray diffraction studies determined the crystalline nature of AgNPs with face-centered cubic (FCC) lattice phase. Dynamic light scattering analysis showed that the biosynthesized AgNPs possess high negative zeta potential value of -35.7 mV. Biosynthesized AgNPs were proved to be potential antioxidants by showing effective radical scavenging activity against 2,2'-diphenyl-1-picrylhydrazyl and H2O2 radicals with IC50 values of 76.95±2.96 and 94.95±2.18 µg/mL, respectively. The biosynthesized AgNPs exhibited significant cytotoxic effects against B16F10 (mouse melanoma, IC50 =26.43±3.41 µg/mL), SKOV3 (human ovarian carcinoma, IC50 =16.24±2.48 µg/mL), A549 (human lung adenocarcinoma, IC50 =39.83±3.74 µg/mL), and PC3 (human prostate carcinoma, IC50 =27.71±2.89 µg/mL) cells. The biosynthesized AgNPs were found to be biocompatible toward normal cells (Chinese hamster ovary cell line, IC50 =438.53±4.2 µg/mL). Cytological observations on most susceptible SKOV3 cells revealed concentration-dependent apoptotic changes that include cell membrane blebbing, cell shrinkage, pyknotic nuclei, karyorrhexis followed by destructive fragmentation of nuclei. The results together in this study strongly provided a base for the development of potential and versatile biomedical applications of biosynthesized AgNPs in the near future. Keywords: AgNPs, Pestalotiopsis microspora, 18S rRNA, SKOV3, B16F10, AO-EB stainingNetala VRBethu MSPushpalatha BBaki VBAishwarya SRao JVTartte VDove Medical PressarticleSilver nanoparticlesPestalotiopsis microsporaAntioxidant activityAnticancer activityHuman ovarian carcinoma SKOV3Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5683-5696 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silver nanoparticles Pestalotiopsis microspora Antioxidant activity Anticancer activity Human ovarian carcinoma SKOV3 Medicine (General) R5-920
spellingShingle	Silver nanoparticles Pestalotiopsis microspora Antioxidant activity Anticancer activity Human ovarian carcinoma SKOV3 Medicine (General) R5-920 Netala VR Bethu MS Pushpalatha B Baki VB Aishwarya S Rao JV Tartte V Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities
description	Vasudeva Reddy Netala,1 Murali Satyanarayana Bethu,2,3 Bobbu Pushpalatha,1 Vijaya Bhaskar Baki,4 Sani Aishwarya,1 J Venkateswara Rao,2 Vijaya Tartte5 1Department of Biotechnology, Sri Venkateswara University, Tirupati, 2Biology Division, Indian Institute of Chemical Technology, Hyderabad, 3Academy of Scientific and Innovative Research, New Delhi, 4Department of Bioinformatics, 5Department of Botany, Sri Venkateswara University, Tirupati, India Abstract: An endophytic fungal strain isolated from the leaves of Gymnema sylvestre was identified as Pestalotiopsis microspora VJ1/VS1 based on nucleotide sequencing of internal transcribed spacer region (ITS 1-5.8S-ITS 2) of 18S rRNA gene (NCBI accession number KX213894). In this study, an efficient and ecofriendly approach has been reported for the synthesis of silver nanoparticles (AgNPs) using aqueous culture filtrate of P. microspora. Ultraviolet-visible analysis confirmed the synthesis of AgNPs by showing characteristic absorption peak at 435 nm. Fourier transform infrared spectroscopy analysis revealed the presence of phenolic compounds and proteins in the fungal filtrate, which are plausibly involved in the biosynthesis and capping of AgNPs. Transmission electron microscopy (TEM) showed that the AgNPs were spherical in shape of 2–10 nm in size. Selected area electron diffraction and X-ray diffraction studies determined the crystalline nature of AgNPs with face-centered cubic (FCC) lattice phase. Dynamic light scattering analysis showed that the biosynthesized AgNPs possess high negative zeta potential value of -35.7 mV. Biosynthesized AgNPs were proved to be potential antioxidants by showing effective radical scavenging activity against 2,2'-diphenyl-1-picrylhydrazyl and H2O2 radicals with IC50 values of 76.95±2.96 and 94.95±2.18 µg/mL, respectively. The biosynthesized AgNPs exhibited significant cytotoxic effects against B16F10 (mouse melanoma, IC50 =26.43±3.41 µg/mL), SKOV3 (human ovarian carcinoma, IC50 =16.24±2.48 µg/mL), A549 (human lung adenocarcinoma, IC50 =39.83±3.74 µg/mL), and PC3 (human prostate carcinoma, IC50 =27.71±2.89 µg/mL) cells. The biosynthesized AgNPs were found to be biocompatible toward normal cells (Chinese hamster ovary cell line, IC50 =438.53±4.2 µg/mL). Cytological observations on most susceptible SKOV3 cells revealed concentration-dependent apoptotic changes that include cell membrane blebbing, cell shrinkage, pyknotic nuclei, karyorrhexis followed by destructive fragmentation of nuclei. The results together in this study strongly provided a base for the development of potential and versatile biomedical applications of biosynthesized AgNPs in the near future. Keywords: AgNPs, Pestalotiopsis microspora, 18S rRNA, SKOV3, B16F10, AO-EB staining
format	article
author	Netala VR Bethu MS Pushpalatha B Baki VB Aishwarya S Rao JV Tartte V
author_facet	Netala VR Bethu MS Pushpalatha B Baki VB Aishwarya S Rao JV Tartte V
author_sort	Netala VR
title	Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities
title_short	Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities
title_full	Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities
title_fullStr	Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities
title_full_unstemmed	Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities
title_sort	biogenesis of silver nanoparticles using endophytic fungus pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/e4c6091f8a994d2f9ce5d928258efb27
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Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities

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